Temporal and Spatial Regulation of Plant Genes
Temporal and Spatial Regulation of Plant Genes

Author: Desh Pal S Verma

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 347

ISBN-13: 370916950X

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First attempts to isolate plant genes were for those genes that are abun dantly expressed in a particular plant organ at a specific stage of devel opment. However, many important gene products are produced in a very minute quantity and in specialized cell types. Such genes can now be isolated using a variety of approaches, some of which are described in this volume. The rapid progress during the last decade in regeneration of a number of crop plants and the availability of molecular tools to introduce foreign genes in plants is allowing the engineering of specific traits of agri cultural importance. These genes must, however, be regulated in a spatial and temporal manner in order to have desired effects on plant devel opment and productivity. The habitat of plants necessitate adaptive responses with respect to the environmental changes. Starting from germination of the seed, the plant begins to sense environmental cues such as moisture, light, temperature and the presence of pathogens, and begins to respond to them. Little is known about various signal transduction pathways that lead to biochemical and morphogenetic responses, in particular, transition from vegetative to reproductive phase. With the availability of tools to generate specific mutations via transposon tagging, identification and isolation of genes affecting these processes may be facilitated. Transfer of these genes into heterologous environments will allow understanding of the complex processes that control plant development.

Jasmonate Signaling
Jasmonate Signaling

Author: Alain Goossens

Publisher: Humana

Published: 2016-08-23

Total Pages: 0

ISBN-13: 9781493960132

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It is now well established that jasmonates, originally identified as the major component of jasmine scent, play a universal role in the plant kingdom and are involved in the regulation of diverse aspects of plant biology, including growth, development, metabolism, and interaction with the environment. In Jasmonate Signaling: Methods and Protocols, experts in the field aim to unite powerful emerging omics platforms with a number of key reductionist approaches to form a comprehensive collection of tools and protocols. The detailed chapters in this book embrace physiological, environmental, molecular, omics, and bioinformatics approaches that allow dissecting jasmonate actions in the model species Arabidopsis thaliana or in other plants. Written in the highly successful Methods in Molecular Biology series format, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, along with tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Jasmonate Signaling: Methods and Protocols will empower interested researchers to dissect all steps of jasmonate signaling and the processes they modulate.

Plant Circadian Networks
Plant Circadian Networks

Author: Dorothee Staiger

Publisher: Humana

Published: 2016-08-23

Total Pages: 0

ISBN-13: 9781493945870

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Plant Circadian Networks: Methods and Protocols provides a collection of protocols to investigate clock-controlled parameters including transcript and small RNA levels, promoter activity using luciferase reporters, protein levels and posttranslational modification, protein-protein interaction, in vivo DNA-protein interaction and RNA-protein interaction, cellular redox state, Ca2+ levels, and innate immune responses. Furthermore, the use of bioinformatics resources is described to evaluate high throughput data sets and to integrate the data into an overarching picture of circadian networks in the cell. Additional chapters focus on seasonal processes like flowering time control, and techniques on trees, moss and algae. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Circadian Networks: Methods and Protocols is designed not only for the chronobiology community dealing with circadian biology but also for the plant community in general.

Plant Promoters and Transcription Factors
Plant Promoters and Transcription Factors

Author: Lutz Nover

Publisher: Springer Science & Business Media

Published: 2013-06-29

Total Pages: 279

ISBN-13: 3540480374

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The control of plant gene expression at the transcriptional level is the main subject of this volume. Genetics, molecular biology and gene technology have dramatically improved our knowledge of this event. The functional analysis of promoters and transcription factors provides more and more insights into the molecular anatomy of initiation complexes assembled from RNA polymerase and the multiplicity of helper and control proteins. Formation of specific DNA-protein complexes - activating or repressing transcription - is the crux of developmental or environmental control of gene expression. The book presents an up-to-date, critical overview of this rapidly advancing field.

Plant RNA Biology
Plant RNA Biology

Author: Dóra Szakonyi

Publisher: Frontiers Media SA

Published: 2019-11-18

Total Pages: 167

ISBN-13: 2889630250

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Discoveries from the past decades revealed that RNA molecules are much more than inert intermediates between the coding DNA sequences and their functional products, proteins. Today, RNAs are recognized as active regulatory molecules influencing gene expression, chromatin organization and genome stability, thus impacting all aspects of plant life including development, growth, reproduction and stress tolerance. Innovations in methodologies, the expanding application of next-generation sequencing technologies, and the creation of public datasets and databases have exposed a new universe of RNA-based mechanisms and led to the discovery of new families of non-coding RNAs, uncovered the large extent of alternative splicing events, and highlighted the potential roles of RNA modifications and RNA secondary structures. Furthermore, considerable advances have been made in identifying RNA-binding and processing factors involved in the synthesis and maturation of different forms of RNA molecules as well as in RNA processing, biochemical modifications or degradation. This Research Topic showcases the broad biological significance of RNAs in plant systems and contains eight original research articles, one review and four mini-reviews, covering various RNA-based mechanisms in higher plants. Emerging new technologies and novel multidisciplinary approaches are empowering the scientific community and will expectedly bring novel insights into our understanding of the mechanisms through which RNA is regulated and regulates biological processes in plant cells.

The Barley Genome
The Barley Genome

Author: Nils Stein

Publisher: Springer

Published: 2018-08-18

Total Pages: 400

ISBN-13: 3319925288

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This book presents an overview of the state-of-the-art in barley genome analysis, covering all aspects of sequencing the genome and translating this important information into new knowledge in basic and applied crop plant biology and new tools for research and crop improvement. Unlimited access to a high-quality reference sequence is removing one of the major constraints in basic and applied research. This book summarizes the advanced knowledge of the composition of the barley genome, its genes and the much larger non-coding part of the genome, and how this information facilitates studying the specific characteristics of barley. One of the oldest domesticated crops, barley is the small grain cereal species that is best adapted to the highest altitudes and latitudes, and it exhibits the greatest tolerance to most abiotic stresses. With comprehensive access to the genome sequence, barley’s importance as a genetic model in comparative studies on crop species like wheat, rye, oats and even rice is likely to increase.

Polarity in Plants
Polarity in Plants

Author: K. Lindsey

Publisher: John Wiley & Sons

Published: 2004

Total Pages: 368

ISBN-13: 9780849323447

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The establishment of polarity is a fundamental feature in eukaryotic development. Polarity in Plants provides an account of current research into the mechanisms by which polarity is generated at the level of the cell, organ and organism in plants, drawing especially on recent work with model organisms. The emphasis is on the use of the techniques of molecular genetics to dissect molecular mechanisms. This is the first volume to bring together the diverse aspects of polarity in plant development.

Plant Systems Biology
Plant Systems Biology

Author: Sacha Baginsky

Publisher: Springer Science & Business Media

Published: 2007-06-25

Total Pages: 362

ISBN-13: 376437439X

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This volume aims to provide a timely view of the state-of-the-art in systems biology. The editors take the opportunity to define systems biology as they and the contributing authors see it, and this will lay the groundwork for future studies. The volume is well-suited to both students and researchers interested in the methods of systems biology. Although the focus is on plant systems biology, the proposed material could be suitably applied to any organism.

Maize Kernel Development
Maize Kernel Development

Author: Brian A Larkins

Publisher: CABI

Published: 2017-11-21

Total Pages: 255

ISBN-13: 178639121X

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This is an authoritative book that acts as a guide to understanding maize kernel development. Written by a team of experts, it covers topics spanning pre- and post-fertilization events, embryo and endosperm development, grain filling and maturation, and factors influencing crop yield. It explores the significance of maize and other cereal grains, existing hypotheses and research, and important gaps in our knowledge and how we might fill them. This is a valuable resource for researchers of maize and other cereals, and anyone working on basic or applied science in the fields of seed development, plant genetics, and crop physiology.

Plant Endosomes
Plant Endosomes

Author: Marisa S. Otegui

Publisher: Humana

Published: 2021-07-21

Total Pages: 200

ISBN-13: 9781071607695

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This second edition details techniques for the study of cargo trafficking through endosomes. New and updated chapters guide readers through methods and protocols on the structural aspects of plant endosomes, combined biochemical, omics, imaging approaches to study the dynamics and contents of endosomal compartments. Additional chapters are dedicated to the analysis of lipids on endosomes and the identification and analysis of lipid binding proteins and lipid-binding domains relevant for the study of plant endosomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Endosomes: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.