Looking at Ribozymes
Looking at Ribozymes

Author: Benoît Masquida

Publisher: John Wiley & Sons

Published: 2024-02-21

Total Pages: 196

ISBN-13: 1394276621

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Behind the neologism “ribozymes” lies a family of fascinating molecules, ribo-enzymes, which have been relatively little studied. These catalytically active RNAs are found in all strata of life, from viruses to the human genome. At the end of the 1970s, the discovery of a catalytic RNA nestled in an intron, followed by another involved in the maturation of transfer RNAs, led to the discovery of new ribozymes and the transition from a strictly “proteocentric” vision, inherited from the dogma of molecular biology, to a more “nucleocentric” one. Since then, a variety of ribozymes have been identified in genomes, where their functions often remain mysterious. Looking at Ribozymes traces the discovery of these molecules and presents a picture of their functional diversity, catalytic mechanisms and distribution within the tree of life.

Looking at Ribozymes
Looking at Ribozymes

Author: Benoît Masquida

Publisher: Iste Press - Elsevier

Published: 2018-06

Total Pages: 130

ISBN-13: 9781785482106

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Looking at Ribozymes: From Atomic to Molecular and Cellular Scales introduces ideas on how ribozymes "work" through a comparison of their common features and specificities. It covers how the (macro) molecular organization at different scales make it possible, what function they carry, where they are found, how they evolved, and how have they been used. The book emphasizes common and specific features of natural and artificial ribozymes, from the basics, to more advanced aspects, making it ideal for undergraduates, grad students and researchers. Specific sections cover different areas of research, from the atomic scale (catalysis), to the molecular (folding, dynamics). In addition, other sections cover macromolecular and cellular scales (dynamics, long-range interactions, self-assembly, cellular processes). The book is not a directory of ribozymes, it instead provides comparative knowledge, including the interdependent relationships between the different scales of description based on different experimental and theoretical approaches. Provides a comparative knowledge of ribozymes based on scales of organization and description Covers different areas of research, from the atomic scale (catalysis), to the molecular (folding, dynamics), macromolecular and cellular scales Includes the interdependent relationships between the different scales of description based on experimental and theoretical approaches

Ribozymes
Ribozymes

Author: Sabine Müller

Publisher: John Wiley & Sons

Published: 2021-07-09

Total Pages: 81

ISBN-13: 3527814531

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Ribozymes Provides comprehensive coverage of a core field in the molecular biosciences, bringing together decades of knowledge from the world’s top professionals in the field Timely and unique in its breadth of content, this all-encompassing and authoritative reference on ribozymes documents the great diversity of nucleic acid-based catalysis. It integrates the knowledge gained over the past 35 years in the field and features contributions from virtually every leading expert on the subject. Ribozymes is organized into six major parts. It starts by describing general principles and strategies of nucleic acid catalysis. It then introduces naturally occurring ribozymes and includes the search for new catalytic motifs or novel genomic locations of known motifs. Next, it covers the development and design of engineered ribozymes, before moving on to DNAzymes as a close relative of ribozymes. The next part examines the use of ribozymes for medicinal and environmental diagnostics, as well as for therapeutic tools. It finishes with a look at the tools and methods in ribozyme research, including the techniques and assays for structural and functional characterization of nucleic acid catalysts. The first reference to tie together all aspects of the multi-faceted field of ribozymes Features more than 30 comprehensive chapters in two volumes Covers the chemical principles of RNA catalysis; naturally occurring ribozymes, engineered ribozymes; DNAzymes; ribozymes as tools in diagnostics and therapy, and tools and methods to study ribozymes Includes first-hand accounts of concepts, techniques, and applications by a team of top international experts from leading academic institutions Dedicates half of its content to methods and practical applications, ranging from bioanalytical tools to medical diagnostics to therapeutics Ribozymes is an unmatched resource for all biochemists, biotechnologists, molecular biologists, and bioengineers interested in the topic.

Ribozymes, 2 Volume Set
Ribozymes, 2 Volume Set

Author: Sabine Müller

Publisher: John Wiley & Sons

Published: 2021-10-18

Total Pages: 81

ISBN-13: 3527344543

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Ribozymes Provides comprehensive coverage of a core field in the molecular biosciences, bringing together decades of knowledge from the world’s top professionals in the field Timely and unique in its breadth of content, this all-encompassing and authoritative reference on ribozymes documents the great diversity of nucleic acid-based catalysis. It integrates the knowledge gained over the past 35 years in the field and features contributions from virtually every leading expert on the subject. Ribozymes is organized into six major parts. It starts by describing general principles and strategies of nucleic acid catalysis. It then introduces naturally occurring ribozymes and includes the search for new catalytic motifs or novel genomic locations of known motifs. Next, it covers the development and design of engineered ribozymes, before moving on to DNAzymes as a close relative of ribozymes. The next part examines the use of ribozymes for medicinal and environmental diagnostics, as well as for therapeutic tools. It finishes with a look at the tools and methods in ribozyme research, including the techniques and assays for structural and functional characterization of nucleic acid catalysts. The first reference to tie together all aspects of the multi-faceted field of ribozymes Features more than 30 comprehensive chapters in two volumes Covers the chemical principles of RNA catalysis; naturally occurring ribozymes, engineered ribozymes; DNAzymes; ribozymes as tools in diagnostics and therapy, and tools and methods to study ribozymes Includes first-hand accounts of concepts, techniques, and applications by a team of top international experts from leading academic institutions Dedicates half of its content to methods and practical applications, ranging from bioanalytical tools to medical diagnostics to therapeutics Ribozymes is an unmatched resource for all biochemists, biotechnologists, molecular biologists, and bioengineers interested in the topic.

Looking at Ribozymes
Looking at Ribozymes

Author: Benoît Masquida

Publisher: John Wiley & Sons

Published: 2024-04-02

Total Pages: 196

ISBN-13: 1786309777

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Behind the neologism “ribozymes” lies a family of fascinating molecules, ribo-enzymes, which have been relatively little studied. These catalytically active RNAs are found in all strata of life, from viruses to the human genome. At the end of the 1970s, the discovery of a catalytic RNA nestled in an intron, followed by another involved in the maturation of transfer RNAs, led to the discovery of new ribozymes and the transition from a strictly “proteocentric” vision, inherited from the dogma of molecular biology, to a more “nucleocentric” one. Since then, a variety of ribozymes have been identified in genomes, where their functions often remain mysterious. Looking at Ribozymes traces the discovery of these molecules and presents a picture of their functional diversity, catalytic mechanisms and distribution within the tree of life.

Ribozymes and RNA Catalysis
Ribozymes and RNA Catalysis

Author: David Malcolm James Lilley

Publisher: Royal Society of Chemistry

Published: 2008

Total Pages: 339

ISBN-13: 0854042539

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Takes the reader through the origins of catalysis in RNA and necessarily includes significant discussion of structure and folding. The main focus of the book concerns chemical mechanism with extensive comment on how, despite the importance of RNA catalysis in the cell, its origins are still poorly understood and often controversial. The reader is given an outline of the important role of RNA catalysis in many aspects of cell function, including RNA processing and translation.

Searching for Ribozymes with New and Improved Properties
Searching for Ribozymes with New and Improved Properties

Author: Edward Arthur Curtis

Publisher:

Published: 2006

Total Pages: 262

ISBN-13:

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(Cont.) Further analysis revealed two groups of mutations, derived from two different ribozymes used to build the shuffled pool, that each increased the rate of the ribozyme by approximately 30-fold. The effects of these mutations were independent of one another, and when combined produced a ribozyme with a rate 600-fold faster than that of the initial isolate. Together, these experiments provide insight into how RNA sequence space can be searched for ribozymes with new and improved properties.

Ribozymes and siRNA protocols
Ribozymes and siRNA protocols

Author: Mouldy Sioud

Publisher: Springer Science & Business Media

Published: 2008-02-03

Total Pages: 623

ISBN-13: 1592597467

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In this completely updated and expanded edition of a classic bench manual, hands-on experts take advantage of the latest advances in ribozyme, DNAzyme, hammerhead ribozymes and derivatives, and RNA interference technologies to describe in detail the exciting and successful methods now available for gene inactivation in vitro and in vivo. Their optimized techniques employ hairpin ribozymes, DNAzymes, hammerhead ribozymes and derivatives, group I intron ribozymes, RNase P ribozymes, and siRNAs, as well as general methods for RNA structure analysis, delivery of oligonucleotides, and gene therapy. Also provided are novel methods for identifying accessible cellular mRNA sites; group I intron and RNAse P ribozyme protocols for effective design, selection, and therapeutic applications; and the latest RNAi methods for sequence-specific gene silencing in a wide variety of organisms. Additional techniques cover the analysis of ribozyme structures and conformational transitions using nucleotide analog interference mapping and fluorescence resonance energy transfer, the use of ribozymes in clinical and gene therapy, and the use of ribozymes and DNAzymes in rodent models of human disease. Each proven protocol includes a background introduction outlining the principle behind the technique, step-by-step instructions, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Ribozymes and siRNA Protocols details for experienced and novice investigators alike the many exciting advances in our understanding of nucleic acid enzymes, as well as demonstrating how they may be used to analyze gene function and target validation, and to productively develop novel therapeutics for human diseases.

Ribozyme Protocols
Ribozyme Protocols

Author: Philip C. Turner

Publisher: Humana

Published: 2013-09-20

Total Pages: 492

ISBN-13: 9781489942852

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The purpose of Ribozyme Protocols is to provide a helpful compilation of protocols that will be of use—^not only to those with some experience of ribozymes—^but also to those wishing to use ribozymes for the first time. Although it is usually impossible to cover every aspect of a scientific field, I believe this book approaches that ideal and should help all readers perform meaningful experiments using ribozymes. To design ribozymes, one must consider whether the target site will be accessible; this task can be facilitated by using computer programs that pre dict the folding of the target RNA. Such programs are detailed in Chapters 2 and 3. If the chosen target is an RNA virus that can mutate rapidly, it makes sense to consider those parts of the genome that are least likely to change during viral replication. An example of how this can be done is described in Chapter 4. Although computer analysis may be a useful starting point to select tar get sites, there seems, at the moment, to be no guarantee that any particular chosen site will be efficiently cleaved. Some workers have deliberately bypassed this problem by using libraries of ribozyme sequences and by select ing those that actually hybridize to and/or cleave the target; these methods are described in Chapters 5 and 6.

Intracellular Ribozyme Applications
Intracellular Ribozyme Applications

Author: John J. Rossi

Publisher: Taylor & Francis

Published: 1999

Total Pages: 294

ISBN-13: 9781898486176

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In this definitive guide to intracellular ribozyme applications, researchers from both industry and academia review the most recent pharmaceutical, therapeutic, and biotechnological applications of ribozymes. Leading scientists from around the world present the latest principles on the application of ribozyme technology to the inhibition of gene expression in living cells. The authors are experts in the field and describe, in detail, protocols for monitoring ribozyme activity, for expressing ribozymes from DNA vectors, for delivering ribozymes to cells, and for in vitro selection of improved ribozymes. The book provides a forward-looking view of ribozyme technology for alteration of gene expression in plants and in treatment of retroviral and genetic diseases in man.