Intrinsic mutagenesis

Intrinsic mutagenesis

Author: Burnet MacFarlane

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 245

ISBN-13: 9401166064

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This book is something which almost accidentally has developed very differently from how it was initially planned. The intention was to elaborate the part played by the immune system in ageing with the role of the thymus as central theme. It was to be essentially an expansion of a lecture I gave in 1970 and would inevitably have been concerned with much the same material as Walford's book, The Immunologic Theory of Aging, though from a different slant. What changed its character arose from a series of attempts to find logical connection between two findings that most gerontologists regard as axiomatic: that the lifespan of a mammal is genetically determined, and that the actual process of ageing is an accumulation of genetic error, of somatic mutations. It is possible that the connection is so indirect, circuitous and multiform that generations of detailed and unattractive research will be needed to elucidate it, or, more likely, the whole matter discarded as a non-problem. But a more inspiring approach does seem possible. The working hypothesis, which halfway through its writing became the new central theme of the book, arose when I was a member of a committee appointed by the Australian Academy of Science at the request of the Australian Government to advise on the danger from French nuclear tests in the South Pacific.


Conditional Mutagenesis: An Approach to Disease Models

Conditional Mutagenesis: An Approach to Disease Models

Author: Robert Feil

Publisher: Springer Science & Business Media

Published: 2007-02-01

Total Pages: 528

ISBN-13: 3540351094

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In this book, leading experts provide timely and comprehensive information on methods for conditional mutagenesis in the mouse and their application to model human physiology and pathophysiology. The book illustrates how sophisticated genetic manipulations of the mouse genome are employed to model human diseases and to identify underlying molecular mechanisms. In addition, it considers the development of new drugs to treat human diseases.


Mutagenesis

Mutagenesis

Author: Rajnikant Mishra

Publisher: BoD – Books on Demand

Published: 2012-08-17

Total Pages: 260

ISBN-13: 9535107070

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The complexity of problem understanding biochemical and molecular basis of healthy life, and eagerness to find simple solution necessitate evolution of technology like mutagenesis. The chapters of this book contain experiences of scientists working in the area of mutagenesis. It describes suitable experimental models (microorganism, plants or animals) for testing spontaneous and induced mutations which are useful for basic and translational research. It includes methods towards gene targeting, developing disease and pest resistant plants, creating temperature sensitive molecular machines, understanding mitochondrial mutagenesis, detecting anti-mutagens, improving genetic insight into impaired immunity and disease. It also describes mutagenesis induced by DNA damage. It has also provided advantage of in vitro transcription and translation to yield proteins with point mutations, deletions or insertions for studying stability, DNA-protein or protein-protein interaction. Trust, it will serve readers as valuable integrated resources emphasizing methods of mutagenesis, and understanding mechanism of variable penetrance or expressivity of mutations.


Advances in Mutagenesis Research 2

Advances in Mutagenesis Research 2

Author: Günter Obe

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 333

ISBN-13: 3642755992

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The new field of applied genetic research, genetic toxicology and mutation research investigates the muta- genicity and cancerogenicity of chemicals and other agents. Permanent changes in genes and chromosomes, or genome mutations, can be induced by a plethora of agents, including ionizing and nonionizing radiations, chemicals, and viruses. Mutagenesis research has two aims: (1) to understand the molecular mechanisms leading to mutations, and (2) to prevent a thoughtless introduction of mutagenic agents into our environment. Both aspects, namely, basic and applied, will be treated in the new series Advances in Mutagenesis Research.


Advances in Mutagenesis Research

Advances in Mutagenesis Research

Author: Günter Obe

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 225

ISBN-13: 3642749550

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The new field of applied genetic research, genetic toxicology and mutation research investigates the mutagenicity and cancerogenicity of chemicals and other agents. Permanent changes in genes and chromosomes, or genome mutations, can be induced by a plethora of agents, including ionizing and nonionizing radiations, chemicals, and viruses. Mutagenesis research has two aims: (1) to understand the molecular mechanisms leading to mutations, and (2) to prevent a thoughtless introduction of mutagenic agents into our environment. Both aspects, namely, basic and applied, will be treated in the new series Advances in Mutagenesis Research.


In Vitro Mutagenesis Protocols

In Vitro Mutagenesis Protocols

Author: Jeff Braman

Publisher: Springer Science & Business Media

Published: 2008-02-05

Total Pages: 284

ISBN-13: 1592591949

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Hands-on researchers with proven track records describe in stepwise fashion their advanced mutagenesis techniques. The contributors focus on improvements to conventional site-directed mutagenesis, including a chapter on chemical site-directed mutagenesis, PCR-based mutagenesis and the modifications that allow high throughput mutagenesis experiments, and mutagenesis based on gene disruption (both in vitro- and in situ-based). Additional methods are provided for in vitro gene evolution; for gene disruption based on recombination, transposon, and casette mutagenesis; and for facilitating the introduction of multiple mutations. Time-tested and highly practical, the protocols in In Vitro Mutagenesis Protocols, 2nd Edition offer today's molecular biologists reliable and powerful techniques with which to illuminate the proteome.


Molecular and Cellular Mechanisms of Mutagenesis

Molecular and Cellular Mechanisms of Mutagenesis

Author: J. Lemontt

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 391

ISBN-13: 1461334764

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It has been nearly 35 years since the peacetime Biology Division of Oak Ridge National Laboratory was started, born of rather inauspicious conditions. Virtually no facilities were available and most of the wartime scientists had left. So, when we started out, it was obvious to me that something had to be done to reestab lish research. Even more, because Oak Ridge was not known at that time for its biological work but rather for the separation of Uranium 235, nuclear reactor development, and radioisotope produc tion, a new tradition had to be promoted. Although good biological work had been done at Oak Ridge during the war to protect the workers and the results of this work were quite excellent, very few installations remained. When we started the work of the Biology Division, it became essential to make it part of the flow of mod~rn biology allover the world. As Director, I had to do more than just attract promising scientists. We created an atmosphere conducive to creative research and nurtured all of the other aspects of a productive laboratory. Of course, we carefully prepared the results of our work in publish able form. We made a sincere effort to invite seminar speakers and lecturers to come to Oak Ridge despite the sacrifices this presented to our early budget. We also had to do something more, and here I "cashed in" on my experience of the previous 15 years.


Stress-Induced Mutagenesis

Stress-Induced Mutagenesis

Author: David Mittelman

Publisher: Springer Science & Business Media

Published: 2013-03-12

Total Pages: 284

ISBN-13: 1461462800

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The discovery of stress-induced mutagenesis has changed ideas about mutation and evolution, and revealed mutagenic programs that differ from standard spontaneous mutagenesis in rapidly proliferating cells. The stress-induced mutations occur during growth-limiting stress, and can include adaptive mutations that allow growth in the otherwise growth-limiting environment. The stress responses increase mutagenesis specifically when cells are maladapted to their environments, i.e. are stressed, potentially accelerating evolution then. The mutation mechanism also includes temporary suspension of post-synthesis mismatch repair, resembling mutagenesis characteristic of some cancers. Stress-induced mutation mechanisms may provide important models for genome instability underlying some cancers and genetic diseases, resistance to chemotherapeutic and antibiotic drugs, pathogenicity of microbes, and many other important evolutionary processes. This book covers pathways of stress-induced mutagenesis in all systems. The principle focus is mammalian systems, but much of what is known of these pathways comes from non-mammalian systems.