Laboratory Exercises and Techniques in Cellular Biology

Laboratory Exercises and Techniques in Cellular Biology

Author: Anthony Contento

Publisher: Wiley Global Education

Published: 2012-10-26

Total Pages: 288

ISBN-13: 1118473957

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The Contento Experimental Cell Biology Lab Book is a modular design that matches the topics discussed in Karp’s textbook. The manual itself consists of 30+ experiments that coincide and complement each of the 18 chapters in the Karp text. There are three possible designs of the lab book, based on the instructor’s needs. These designs focus on either Techniques, Concepts, or Organelles. The procedures of the 30+ experiments remain standard and unchanged in all designs of the lab book. Special Overview pages, Discussion Questions and Datasheets bookend the procedures in order to create each of the possible textbook designs.This gives instructors flexibility to create a lab book that suits their lecture course curriculum, their experience, and available equipment and supplies.


Molecular Biology Techniques

Molecular Biology Techniques

Author: Heather B. Miller

Publisher: Academic Press

Published: 2011-10-18

Total Pages: 227

ISBN-13: 0123855454

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This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. - Cover basic concepts and techniques used in molecular biology research labs - Student-tested labs proven successful in a real classroom laboratories - Exercises simulate a cloning project that would be performed in a real research lab - "Project" approach to experiments gives students an overview of the entire process - Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions


The Biology of Exercise

The Biology of Exercise

Author: Michael J. Joyner

Publisher: Perspectives Cshl

Published: 2017

Total Pages: 408

ISBN-13: 9781621821656

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Exercise training provokes widespread transformations in the human body, requiring coordinated changes in muscle composition, blood flow, neuronal and hormonal signaling, and metabolism. These changes enhance physical performance, improve mental health, and delay the onset of aging and disease. Understanding the molecular basis of these changes is therefore important for optimizing athletic ability and for developing drugs that elicit therapeutic effects. Written and edited by experts in the field, this collection from Cold Spring Harbor Perspectives in Medicine examines the biological basis of exercise from the molecular to the systemic levels. Contributors discuss how transcriptional regulation, cytokine and hormonal signaling, glucose metabolism, epigenetic modifications, microRNA profiles, and mitochondrial and ribosomal functions are altered in response to exercise training, leading to improved skeletal muscle, hippocampal, and cardiovascular function. Cross talk among the pathways underlying tissue-specific and systemic responses to exercise is also considered. The authors also discuss how the understanding of such molecular mechanisms may lead to the development of drugs that mitigate aging and disease. This volume will therefore serve as a vital reference for all involved in the fields of sports science and medicine, as well as anyone seeking to understand the molecular mechanisms by which exercise promotes whole-body health.


Laboratory Exercises in Developmental Biology

Laboratory Exercises in Developmental Biology

Author: Yolanda P. Cruz

Publisher: Academic Press

Published: 2012-12-02

Total Pages: 254

ISBN-13: 0323137776

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This intensive manual provides students with valuable information and insights into animal development at the organismal, cellular, and subcellular levels. The book uses both descriptive and investigative approaches that emphasize techniques, key experiments, and data analysis. - Provides a broad introductory view of developmental systems - Teaches both classical embryology and modern experimental approaches - Contains seventeen laboratory exercises, written in step-by-step style - Organized with additional notes to students and preparators - Lists questions and references for each exercise - Special chapters give introductions to the scientific process, use of the microscope, and the writing of scientific papers - Illustrated with detailed line drawings


Molecular and Cellular Regulation of Adaptation to Exercise

Molecular and Cellular Regulation of Adaptation to Exercise

Author: Claude Bouchard

Publisher: Academic Press

Published: 2015-11-16

Total Pages: 588

ISBN-13: 0128039922

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Molecular Aspects of Exercise Biology and Exercise Genomics, the latest volume in the Progress in Molecular Biology and Translational Science series includes a comprehensive summary of the evidence accumulated thus far on the molecular and cellular regulation of the various adaptations taking place in response to exercise. Changes in the cellular machinery are described for multiple tissues and organs in terms of signaling pathways, gene expression, and protein abundance. Adaptations to acute exercise as well as exposure to regular exercise are also discussed and considered. - Includes a comprehensive summary of the evidence accumulated thus far on the molecular and cellular regulation of the various adaptations taking place in response to exercise - Contains contributions from leading authorities - Informs and updates on all the latest developments in the field of exercise biology and exercise genomics


Genetics and Molecular Biology of Muscle Adaptation

Genetics and Molecular Biology of Muscle Adaptation

Author: Neil Spurway

Publisher: Elsevier Health Sciences

Published: 2006-01-01

Total Pages: 285

ISBN-13: 0443100772

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This title is directed primarily towards health care professionals outside of the United States. It starts with the origin of life and ends with the mechanisms that make muscles adapt to different forms of training. In between, it considers how evidence has been obtained about the extent of genetic influence on human capacities, how muscles and their fibres are studied for general properties and individual differences, and how molecular biological techniques have been combined with physiological ones to produce the new discipline of molecular exercise physiology. This is the first book on such topics written specifically for modules in exercise and sport science at final year Hons BSc and taught MSc levels.


Experiments in Molecular Biology

Experiments in Molecular Biology

Author: Zachary F. Burton

Publisher: Academic Press

Published: 1997-02-20

Total Pages: 252

ISBN-13: 9780121473709

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Experiments in Molecular Biology provides a thorough introduction to recombinant DNA methods used in molecular biology and nucleic acid biochemistry. This unique laboratory manual is particularly appropriate for courses in molecular cloning, molecular genetics techniques, molecular biology techniques, recombinant DNA techniques, bacterial genetics techniques, and genetic engineering. Included is an especially helpful section to aid new instructors in avoiding potential pitfalls of specific experiments. Key Features * Contains student-tested, easy-to-follow protocols * Presents background information that reinforces principles behind the methods presented * Includes questions at the end of laboratory exercises * Provides both detailed descriptions of experimental procedures and a theoretical support section * Sequentially links experiments to provide a "project" approach to studying molecular biochemistry * Includes student-tested, easy-to-follow protocols * Background information reinforces principles behind the methods presented * Includes questions at the end of laboratory exercises * Advises new instructors on potential pitfalls of specific experiments * Provides both detailed descriptions of experimental procedures and a theoretical support section * Sequentially links experiments to provide a "project" approach to studying


Environmental Laboratory Exercises for Instrumental Analysis and Environmental Chemistry

Environmental Laboratory Exercises for Instrumental Analysis and Environmental Chemistry

Author: Frank M. Dunnivant

Publisher: John Wiley & Sons

Published: 2004-11-26

Total Pages: 416

ISBN-13: 0471660272

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A comprehensive set of real-world environmental laboratoryexperiments This complete summary of laboratory work presents a richlydetailed set of classroom-tested experiments along with backgroundinformation, safety and hazard notes, a list of chemicals andsolutions needed, data collection sheets, and blank pages forcompiling results and findings. This useful resource also: Focuses on environmental, i.e., "dirty" samples Stresses critical concepts like analysis techniques anddocumentation Includes water, air, and sediment experiments Includes an interactive software package for pollutant fate andtransport modeling exercises Functions as a student portfolio of documentationabilities Offers instructors actual samples of student work fortroubleshooting, notes on each procedure, and procedures forsolutions preparation.