Differential-Display Reverse Transcription-PCR (DDRT-PCR)
Differential-Display Reverse Transcription-PCR (DDRT-PCR)

Author: Sergio Colonna-Romano

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 135

ISBN-13: 3642804543

DOWNLOAD EBOOK

Identification of differentially expressed genes is one of the major challenges in molecular biology. Several techniques allow the cloning of such sequences. However, methods such as RNA subtraction or differential hybridization are time-consuming and require large amounts of mRNA. Recently, a new approach has successfully been developed: Differential-Display Reverse Transcription-PCR (DDRT-PCR). This technique has been proven to be highly effective in identifying sequences that are differentially expressed in various cell types. The most striking advantage is, however, that only nanograms of total RNA are sufficient. Thus every mRNA species expressed in the cell system can be investigated, even those present at very low levels.

Differential Display Methods and Protocols
Differential Display Methods and Protocols

Author: Peng Liang

Publisher: Springer Science & Business Media

Published: 2008-02-04

Total Pages: 321

ISBN-13: 1592599680

DOWNLOAD EBOOK

Since the first edition of this book dedicated to differential display (DD) technology was published in 1997, we have witnessed an explosive interest in studying differential gene expression. The gene-hunting euphoria was initially powered by the invention of DD, which was gradually overtaken by DNA microarray technology in recent years. Then why is there still the need for second edition of this DD book? First of all, DD still enjoys a substantial lead over DNA microarrays in the ISI citation data (see Table 1), despite the h- dreds of millions of dollars spent each year on arrays. This may come as a surprise to many, but to us it implies that many of the DNA microarray studies went unpublished owing to their unfulfilled promises (1). Second, unlike DNA microarrays, DD is an “open”-ended gene discovery method that does not depend on prior genome sequence information of the organism being studied. As such, DD is applicable to the study of all living organisms—from bacteria, fungi, insects, fish, plants, to mammals—even when their genomes are not sequenced. Second, DD is more accessible technically and financially to most cost-conscious “cottage-industry” academic laboratories. So clearly DD still has its unique place in the modern molecular biological toolbox for gene expression analysis.

Differential Display
Differential Display

Author: Ron A. Leslie

Publisher: OUP Oxford

Published: 2000-05-04

Total Pages: 282

ISBN-13: 0191565946

DOWNLOAD EBOOK

One of the most challenging tasks facing the modern biological research laboratory is to make sense of the enormous amount of data being generated by various genome projects currently underway, and especially the human genome project. Understanding the ways in which genes are differentially expressed in various tissues and cell types, throughout ontogenetic development and in pathological processes, will go a long way towards understanding the function of all these 'new' genes and their protein products. Differential Display explains in detail how to perform the technique of RT-PCR Differential Display in various kinds of experimental biological systems. It also examines this technique in the context of other methods of studying differential gene expression such as subtractive hybridisation and the use of high-density gene microarrays combined with hybridisation techniques and automatic image analysis.

RNA Methodologies
RNA Methodologies

Author: Robert E. Farrell Jr.

Publisher: Elsevier

Published: 2010-07-22

Total Pages: 794

ISBN-13: 0080454763

DOWNLOAD EBOOK

This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project.RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5' and 3' RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis.* Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects

Advances in Animal Genomics
Advances in Animal Genomics

Author: Sukanta Mondal

Publisher: Academic Press

Published: 2020-11-25

Total Pages: 340

ISBN-13: 0128206128

DOWNLOAD EBOOK

Advances in Animal Genomics provides an outstanding collection of integrated strategies involving traditional and modern - omics (structural, functional, comparative and epigenomics) approaches and genomics-assisted breeding methods which animal biotechnologists can utilize to dissect and decode the molecular and gene regulatory networks involved in the complex quantitative yield and stress tolerance traits in livestock. Written by international experts on animal genomics, this book explores the recent advances in high-throughput, next-generation whole genome and transcriptome sequencing, array-based genotyping, and modern bioinformatics approaches which have enabled to produce huge genomic and transcriptomic resources globally on a genome-wide scale. This book is an important resource for researchers, students, educators and professionals in agriculture, veterinary and biotechnology sciences that enables them to solve problems regarding sustainable development with the help of current innovative biotechnologies. - Integrates basic and advanced concepts of animal biotechnology and presents future developments - Describes current high-throughput next-generation whole genome and transcriptome sequencing, array-based genotyping, and modern bioinformatics approaches for sustainable livestock production - Illustrates integrated strategies to dissect and decode the molecular and gene regulatory networks involved in complex quantitative yield and stress tolerance traits in livestock - Ensures readers will gain a strong grasp of biotechnology for sustainable livestock production with its well-illustrated discussion

Tensors, Differential Forms, and Variational Principles
Tensors, Differential Forms, and Variational Principles

Author: David Lovelock

Publisher: Courier Corporation

Published: 2012-04-20

Total Pages: 402

ISBN-13: 048613198X

DOWNLOAD EBOOK

Incisive, self-contained account of tensor analysis and the calculus of exterior differential forms, interaction between the concept of invariance and the calculus of variations. Emphasis is on analytical techniques. Includes problems.

Differential Forms and Connections
Differential Forms and Connections

Author: R. W. R. Darling

Publisher: Cambridge University Press

Published: 1994-09-22

Total Pages: 288

ISBN-13: 9780521468008

DOWNLOAD EBOOK

Introducing the tools of modern differential geometry--exterior calculus, manifolds, vector bundles, connections--this textbook covers both classical surface theory, the modern theory of connections, and curvature. With no knowledge of topology assumed, the only prerequisites are multivariate calculus and linear algebra.

Differential Topology
Differential Topology

Author: Morris W. Hirsch

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 230

ISBN-13: 146849449X

DOWNLOAD EBOOK

"A very valuable book. In little over 200 pages, it presents a well-organized and surprisingly comprehensive treatment of most of the basic material in differential topology, as far as is accessible without the methods of algebraic topology....There is an abundance of exercises, which supply many beautiful examples and much interesting additional information, and help the reader to become thoroughly familiar with the material of the main text." —MATHEMATICAL REVIEWS

Topology from the Differentiable Viewpoint
Topology from the Differentiable Viewpoint

Author: John Willard Milnor

Publisher: Princeton University Press

Published: 1997-12-14

Total Pages: 80

ISBN-13: 9780691048338

DOWNLOAD EBOOK

This elegant book by distinguished mathematician John Milnor, provides a clear and succinct introduction to one of the most important subjects in modern mathematics. Beginning with basic concepts such as diffeomorphisms and smooth manifolds, he goes on to examine tangent spaces, oriented manifolds, and vector fields. Key concepts such as homotopy, the index number of a map, and the Pontryagin construction are discussed. The author presents proofs of Sard's theorem and the Hopf theorem.