Molecular Biology of the Cell
Author:
Publisher:
Published: 2002
Total Pages: 0
ISBN-13: 9780815332183
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A Laboratory Guide to the Tight Junction
Author: Jianghui Hou
Publisher: Academic Press
Published: 2020-04-24
Total Pages: 422
ISBN-13: 0128226439
DOWNLOAD EBOOKA Laboratory Guide to the Tight Junction offers broad coverage of the unique methods required to investigate its characteristics. The methods are described in detail, including its biochemical and biophysical principles, step-by-step process, data analysis, troubleshooting, and optimization. The coverage includes various cell, tissue, and animal models. Chapter 1 provides the foundations of cell biology of tight junction. Chapter 2 covers the Biochemical approaches for paracellular channels and is followed by chapter 3 providing the Biophysical approaches. Chapter 4 describes and discusses Histological approaches for tissue fixation and preparation. Chapter 5 discusses Light microscopy, while chapter 6 presents Electron microscopic approaches. Chapter 7 covers Transgenic manipulation in cell cultures, including DNA and siRNA, Mutagenesis, and viral infection. Chapter 8 covers transgenic manipulation in mice, including: Knockout, Knockin, siRNA knockdown, GFP/LacZ reporter, and overexpression. The final chapter discusses the future developments of new approaches for tight junction research. Researchers and advanced students in bioscience working on topics of cell junction, ion channel and membrane protein will benefit from the described methods. Clinicians and pathologists interested in tissue barrier diseases will also benefit from the biochemical and biophysical characterization of tight junctions in organ systems, and their connection to human diseases. - Provides consistent and detailed research methods - Covers various cell, tissue and animal models - Includes step-by-step guidance from beginner to sophisticated levels
Tight Junctions in Cancer Metastasis
Author: Tracey A. Martin
Publisher: Springer Science & Business Media
Published: 2013-02-26
Total Pages: 315
ISBN-13: 9400760280
DOWNLOAD EBOOKThere has been a dramatic increase in knowledge of tight junctions in the past decade. The molecular structure of tight junctions, cellular functions and the pathophysiological roles of tight junctions are becoming clear. Of the most important functions, the role of the cellular structure in cancer spread and drug delivery are increasingly realised. It is now clear that there are fundamental changes to tight junctions during the process of cancer development. Tight junctions are also critical to the metastatic process of cancer cells. The cellular structure is also crucial in drug therapies, namely, the permeability and bioavailability of the drugs, penetration of barriers such as the blood brain barrier. This current volume aims to summarise the current knowledge of tight junctions, their role in cancer and cancer metastasis and is of interest to scientists and clinicians.
Tight Junctions
Author: Lorenza González-Mariscal
Publisher: Springer Science & Business Media
Published: 2007-02-26
Total Pages: 235
ISBN-13: 0387366733
DOWNLOAD EBOOKThis book provides state-of-the-art insight into Tight Junctions (TJs), the intersection of epithelial and endothelial cells in various tissues. Opening with an analysis of the evolutionary development of a transporting epithelium in early metazoans, the book describes current understanding of TJ structure and function information, including evidence supporting the presence of ion channels within TJs. Also deals with TJs of central nervous system myelin and at the blood brain barrier.
Cells: Molecules and Mechanisms
Author: Eric Wong
Publisher: Axolotl Academic Publishing
Published: 2009
Total Pages: 283
ISBN-13: 0985226110
DOWNLOAD EBOOK"Yet another cell and molecular biology book? At the very least, you would think that if I was going to write a textbook, I should write one in an area that really needs one instead of a subject that already has multiple excellent and definitive books. So, why write this book, then? First, it's a course that I have enjoyed teaching for many years, so I am very familiar with what a student really needs to take away from this class within the time constraints of a semester. Second, because it is a course that many students take, there is a greater opportunity to make an impact on more students' pocketbooks than if I were to start off writing a book for a highly specialized upper- level course. And finally, it was fun to research and write, and can be revised easily for inclusion as part of our next textbook, High School Biology."--Open Textbook Library.
The Cerebral Circulation
Author: Marilyn J. Cipolla
Publisher: Biota Publishing
Published: 2016-07-28
Total Pages: 82
ISBN-13: 1615047239
DOWNLOAD EBOOKThis e-book will review special features of the cerebral circulation and how they contribute to the physiology of the brain. It describes structural and functional properties of the cerebral circulation that are unique to the brain, an organ with high metabolic demands and the need for tight water and ion homeostasis. Autoregulation is pronounced in the brain, with myogenic, metabolic and neurogenic mechanisms contributing to maintain relatively constant blood flow during both increases and decreases in pressure. In addition, unlike peripheral organs where the majority of vascular resistance resides in small arteries and arterioles, large extracranial and intracranial arteries contribute significantly to vascular resistance in the brain. The prominent role of large arteries in cerebrovascular resistance helps maintain blood flow and protect downstream vessels during changes in perfusion pressure. The cerebral endothelium is also unique in that its barrier properties are in some way more like epithelium than endothelium in the periphery. The cerebral endothelium, known as the blood-brain barrier, has specialized tight junctions that do not allow ions to pass freely and has very low hydraulic conductivity and transcellular transport. This special configuration modifies Starling's forces in the brain microcirculation such that ions retained in the vascular lumen oppose water movement due to hydrostatic pressure. Tight water regulation is necessary in the brain because it has limited capacity for expansion within the skull. Increased intracranial pressure due to vasogenic edema can cause severe neurologic complications and death.
Biology and Regulation of Blood‐Tissue Barriers
Author: C. Yan Cheng
Publisher: Springer Science & Business Media
Published: 2014-01-25
Total Pages: 380
ISBN-13: 1461447119
DOWNLOAD EBOOKThis book was written by many outstanding investigators who have spent decades to study different aspects of blood‐tissue barrier function. They have summarized some of the latest and fascinating development in their fields of research including the blood‐brain barrier, the blood‐retinal barrier, the gut barrier, the blood‐biliary barrier, the blood‐follicle barrier, the blood‐epididymis barrier, the blood‐testis barrier, the tight junction barrier in general as well as barriers in the female reproductive tract. Included are also chapters that focus on topics that are physiologically applicable to all blood‐tissue barriers. Many of these chapters also include information on specific human diseases, such as pathological changes of the gut barrier that cause bowel disorders resulting from inflammation of the epithelial lining in the intestine, and infertility in men as a result of disruption of the blood‐epididymal and/or blood‐testis barriers; and on new therapeutic approaches (e.g., drug delivery across the blood‐brain and the blood‐retinal barriers).
Tight Junctions
Author: Marcelino Cereijido
Publisher: CRC Press
Published: 2001-06-26
Total Pages: 793
ISBN-13: 1420038532
DOWNLOAD EBOOKAs the first detailed overview of the subject, Tight Junctions brought together diverse perspectives from leading investigators to provide a multidisciplinary overview of the field. Since its publication in 1992, there has been an explosion of information about tight junctions, particularly in the area of molecular structure, cell and molecular bio
Current Topics in Membranes
Author:
Publisher: Academic Press
Published: 1992-01-08
Total Pages: 477
ISBN-13: 0080585108
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Skin Barrier Function
Author: T. Agner
Publisher: Karger Medical and Scientific Publishers
Published: 2016-02-04
Total Pages: 172
ISBN-13: 3318055867
DOWNLOAD EBOOKAlthough a very fragile structure, the skin barrier is probably one of the most important organs of the body. Inward/out it is responsible for body integrity and outward/in for keeping microbes, chemicals, and allergens from penetrating the skin. Since the role of barrier integrity in atopic dermatitis and the relationship to filaggrin mutations was discovered a decade ago, research focus has been on the skin barrier, and numerous new publications have become available. This book is an interdisciplinary update offering a wide range of information on the subject. It covers new basic research on skin markers, including results on filaggrin and on methods for the assessment of the barrier function. Biological variation and aspects of skin barrier function restoration are discussed as well. Further sections are dedicated to clinical implications of skin barrier integrity, factors influencing the penetration of the skin, influence of wet work, and guidance for prevention and saving the barrier. Distinguished researchers have contributed to this book, providing a comprehensive and thorough overview of the skin barrier function. Researchers in the field, dermatologists, occupational physicians, and related industry will find this publication an essential source of information.
