Read and Download eBook Full
Author: M. N. Gupta
Publisher:
Published: 1993
Total Pages: 232
ISBN-13:
DOWNLOAD EBOOKThis text provides information on thermostability of enzymes. It includes topics such as: structure, stability, isolation and purification of proteins; thermophilic microorganisms; models of enzyme deactivation; and chemical modification and crosslinking for enhancing thermostability for enzymes.
Author: Thomas D. Brock
Publisher: Wiley-Interscience
Published: 1986
Total Pages: 336
ISBN-13:
DOWNLOAD EBOOKDealing with an important and interesting group of microorganisms, those capable of living at very high temperatures, this book discusses the biochemical, molecular and genetic properties of these organisms, examining their potential biotechnological uses in both industry and agriculture - in particular, in the production of fuels and chemicals, in waste treatment systems, and for mining. The book provides a concise overview of the group and gives useful illustrations of high temperature habitats, and contains the latest information on molecular genetics.
Author: Richard Bott
Publisher: Springer Science & Business Media
Published: 2013-11-11
Total Pages: 287
ISBN-13: 1461303192
DOWNLOAD EBOOKSubtilisin is the most extensively studied model system for protein engineering. The primary motivating factor for the interest in subtilisin is the commercial utility of this class of proteases. The subtilisin symposium was the first international meeting to bring together a large number of groups that have focused on the subtilisins and the subtilases-the protein superfamily of subtilisin-like enzymes. The results presented at the symposium are in this way a unique compendium of a broad spectrum of work largely focused on harnessing the potential of site-directed mutagenesis to understand and deliberately alter the function of these enzymes toward a desired end. This sort of protein engineering has been extremely successful in subtilisin, with many such "engineered" enzymes now widely used in commer cial enterprises. In this regard the experience derived from subtilisin does represent practical protein engineering. It is becoming clear that subtilisin represents a larger class of enzymes, the subtilases, that include many of the human pro hormone-converting enzymes. As international collabo rative efforts to sequence entire genomes continue, we can reasonably expect that additional members of the subtilase class will be encountered. Whenever interest in a member of this class of enzyme arises, the work on subtilisin will serve as a guide to the analysis for what in bacillus, fungi, and industry is an everyday workhorse enzyme.
Author: Srikanta Sen
Publisher: CRC Press
Published: 2016-04-19
Total Pages: 184
ISBN-13: 143983914X
DOWNLOAD EBOOKThis book covers the basic structural, thermodynamic and kinetic principles are covered and molecular strategies for the adaptation to high temperatures revealed by structure analysis are delineated. The roles of fluctuations, hydration and internal packing are thoroughly dicussed. Enzymes with a particular industrial importance, the subtilisin-like serine proteases, have been extensively studied by protein engineering. One extensive chapter is devoted to the present state of knowledge concerning structure-function relations and the origin of the their structural stability. Last but not least, computational and experimental approaches for the design of proteins with increased thermal stability based on sequences or 3D structures are present
Author: Noha M. Mesbah
Publisher: Frontiers Media SA
Published: 2016-08-05
Total Pages: 105
ISBN-13: 2889198308
DOWNLOAD EBOOKEnzymes are nature’s biocatalysts empowered with high catalytic power and remarkable substrate specificity. Enzymes perform a wide range of functions throughout nature, and guide the biochemistry of life with great precision. The majority of enzymes perform under conditions considered normal for mesophilic, neutrophilic, terrestrial microorganisms. However, the Earth’s biosphere contains several regions that are extreme in comparison, such as hypersaline lakes and pools, hydrothermal vents, cold oceans, dry deserts and areas exposed to intensive radiation. These areas are inhabited by a large number of extremophilic microorganisms which produce enzymes capable of functioning in unusual conditions. There is an increasing biotechnological and industrial demand for enzymes stable and functioning in harsh conditions, and over the past decade screening for, isolation and production of enzymes with unique and extreme properties has become one of the foremost areas of biotechnology research. The development of advanced molecular biology tools has facilitated the quest for production of enzymes with optimized and extreme features. These tools include large-scale screening for potential genes using metagenomics, engineering of enzymes using computational techniques and site-directed mutagenesis and molecular evolution techniques. The goal of this Research Topic is to present reports on latest advances in enzymes from all types of extreme environments. Contributions dealing with isolation of enzymes from extremophilic microorganisms or directly from natural environments, screening for and expression of enzymes with extreme properties using metagenomic approaches are welcome. In addition, contributions dealing with all forms of biocatalyst production and improvement are welcome, such as fermentation technology, protein engineering, directed evolution, rational design, and immobilization techniques.
Author: Tulasi Satyanarayana
Publisher: Springer Science & Business Media
Published: 2013-03-25
Total Pages: 951
ISBN-13: 9400758995
DOWNLOAD EBOOKThe existence of life at high temperatures is quiet fascinating. At elevated temperatures, only microorganisms are capable of growth and survival. Many thermophilic microbial genera have been isolated from man-made (washing machines, factory effluents, waste streams and acid mine effluents) and natural (volcanic areas, geothermal areas, terrestrial hot springs, submarine hydrothermal vents, geothermally heated oil reserves and oil wells, sun-heated litter and soils/sediments) thermal habitats throughout the world. Both culture-dependent and culture-independent approaches have been employed for understanding the diversity of microbes in hot environments. Interest in their diversity, ecology, and physiology has increased enormously during the past few decades as indicated by the deliberations in international conferences on extremophiles and thermophiles held every alternate year and papers published in journals such as Extremophiles. Thermophilic moulds and bacteria have been extensively studied in plant biomass bioconversion processes as sources of industrial enzymes and as gene donors. In the development of third generation biofuels such as bioethanol, thermophilic fungal and bacterial enzymes are of particular interest. The book is aimed at bringing together scattered up-to-date information on various aspects of thermophiles such as the diversity of thermophiles and viruses of thermophiles, their potential roles in pollution control and bioremediation, and composting.
Author: Uwe Theo Bornscheuer
Publisher: John Wiley & Sons
Published: 2006-05-12
Total Pages: 368
ISBN-13: 3527607129
DOWNLOAD EBOOKFrom reviews to the first edtion: "Bornscheuer and Kazlauskas have set out, and succeeded, in producing a definitive manual on hydrolytic enzymes (especially lipases, esterases, and proteases) for organic chemists. This is quite simply the best book of its type and can be unreservedly recommended to organic chemists who have an interest in using hydrolytic enzymes in synthesis." (Nicholas J. Turner, University of Edinburgh) "The book is an indispensable source of information on the use of hydrolases in organic synthesis. The subject matter is very well set out, and the chapters are clearly written and presented from a critical viewpoint. Bornscheuer and Kazlauskas have succeeded admirably in describing the capabilities and limitations of the use of hydrolytic enzymes and in critically evaluating them. No library should be without the book." (Fritz Theil, WITEGA Angewandte Werkstoff-Forschung GmbH, Berlin) The second edition of this extremely successful and well-proven book presents recent developments in the use of hydrolases for organic synthesis, reflecting in particular the enormous progress made in enzyme discovery and optimization with a new chapter on "Protein Sources and Optimization of Biocatalyst Performance". The renowned authors survey the stereoselective reactions of hydrolases, especially lipases, esterases and proteases, giving researchers an overview of what has worked in the past so that they can judge how to solve their own synthetic problems. In total, the book contains over one thousand chemical structures, rounded off by some 1,800 invaluable references.
Author: Young Je Yoo
Publisher: Springer
Published: 2017-01-12
Total Pages: 208
ISBN-13: 9402410260
DOWNLOAD EBOOKThis book provides a comprehensive introduction to all aspects of enzyme engineering, from fundamental principles through to the state-of-the-art in research and industrial applications. It begins with a brief history, describing the milestones of advancement in enzyme science and technology, before going on to cover the fundamentals of enzyme chemistry, the biosynthesis of enzymes and their production. Enzyme stability and the reaction kinetics during enzymatic reactions are presented to show how enzymes function during catalysis and the factors that affect their activity. Methods to improve enzyme performance are also presented, such as cofactor regeneration and enzyme immobilization. The book emphasizes and elaborates on the performance and characteristics of enzymes at the molecular level. Finally, the book presents recent advances in enzyme engineering and some key industrial application of enzymes addressing the present needs of society. This book presents essential information not only for undergraduate and graduate students, but also for researchers in academia and industry, providing a valuable reference for the development of commercial applications of enzyme technology.
Author: Gonzalo de Gonzalo
Publisher: John Wiley & Sons
Published: 2021-07-19
Total Pages: 54
ISBN-13: 352734683X
DOWNLOAD EBOOKThis reference book originates from the interdisciplinary research cooperation between academia and industry. In three distinct parts, latest results from basic research on stable enzymes are explained and brought into context with possible industrial applications. Downstream processing technology as well as biocatalytic and biotechnological production processes from global players display the enormous potential of biocatalysts. Application of "extreme" reaction conditions (i.e. unconventional, such as high temperature, pressure, and pH value) - biocatalysts are normally used within a well defined process window - leads to novel synthetic effects. Both novel enzyme systems and the synthetic routes in which they can be applied are made accessible to the reader. In addition, the complementary innovative process technology under unconventional conditions is highlighted by latest examples from biotech industry.
Author: Elizabeth van Pelt-Verkuil
Publisher: Springer Science & Business Media
Published: 2008-03-14
Total Pages: 333
ISBN-13: 1402062419
DOWNLOAD EBOOKKary Mullis was awarded a Nobel Prize for inventing the PCR technique more than a decade ago in 1993. Since its "discovery", multiple adaptations and variations of the standard PCR technique have been described. This publication aims to provide the reader with a guide to the standard PCR technique and its many available variants, with particular emphasis being placed on the role of these PCR techniques in the clinical diagnostic laboratory (the central theme of this book).
