New data on the pollen tube reflects its qualities as a biological model beyond its function as a carrier of sperm cells in plant reproduction. This book shows that pollen tubes are excellent models for plant cell research, suitable for investigations on cell tip growth and polarization, signal transduction, channel and ion flux activity, gene expression, cytoskeleton and wall structure, membrane dynamics and even cell–to-cell communication.
The subject of this volume is the reproductive biology of plants. A steadily growing interest in this field is the result of at least two factors, as pointed out with great foresight by one of the driving forces in the field, H.E Linskens (Linskens 1964): most of the food consumed by humans takes the form of plant reproductive parts, and molecular biology now provides pow erful tools for investigating and manipulating plant reproductive systems. Molecular biology and the allied discipline of biotechnology are solidly represented in the papers in this book. The editors of Angiosperm Pollen and Ovules believe that the chapters herein contain some of the most excit ing findings of contemporary biology, and hope that the readers of this book will share their enthusiasm. The editors express sincere and grateful thanks for help from Carla Frova, Enrico Pe, and especially to Giorgio Binelli, all of the University of Milan. Without these three tireless and enthusiastic individuals it would not have been possible to maintain the apparently effortless proceeding of this congress. We thank also the organizing committee and the organizations that generously provided financial support (both listed below). Finally, we extend thanks to M. Cresti, D. Charlesworth, D. Hess, E Hoekstra, R. Bruce Knox, J.P. Mascarenhas, M.E. Nasrallah, P.L. Pfahler, A. Snow, and M.T.M. Willemse for chairing sessions.
In recent years there has been a growing awareness of the importance of reproductive biology to crop production and there has been a tremendous increase in research on reproductive structures of higher plants. Presented here is a wide information of different aspects of micro- and macrosporogenesis, pollen-stigma interaction and recognition, pollen tube growth, cytoskeleton, in vitro and in vivo gamete fusion, and incompatibility. The most advanced techniques employed in studies on reproductive biology of higher plants are described in detail.
Pollen, the plant structure most widely used by humans, is a key structure in plant reproduction giving rise to fruits and seeds. Moreover, the biotechnological use of pollen is of great importance for plant breeders since it allows to obtain varieties with better utilization and yield. In the first part, the successive steps of pollen development in the anther from floral induction to pollen germination and fertilization are thoroughly examined; the second part is devoted to pollen behaviour in vitro.
Plants are the basic source of food for both humans and animals. Most of the food is made of fruits and seeds. For these to be formed, pollination must first take place. This process is the transfer of pollen grains from the anther, which is the male structure of the flower, to the sigma on the female structure of the flower. The transfer process requires agents to be carried out. The agents can be either biotic or abiotic. Nature perfected this arrangement between the pollination agents and the plants. As ecosystems and agricultural systems are changing, this balanced arrangement becomes disturbed. This makes it necessary that pollination systems be studied so that necessary measures can be undertaken to ensure productivity. The chapters of this book present results in research undertaken to improve productivity in crops such as Actinidia chinensis (the kiwifruit), Theobroma cacao (cocoa), and Manicaria saccifera (a tropical forest palm). Some results are presented on tests to check the viability of pollen grains and the delivery of sperm cells through pollen tubes to the embryo sac. These results can serve as guidelines to any person seeking to improve pollination and productivity or to check the efficiency on pollination in ecosystems or agricultural production systems.
The author offers an overview of pollen biology and biotechnology for students and researchers in areas such as reproductive biology, biotechnology, aeropalynology, plant breeding, horticulture, and forestry. Citing more than 1,500 references to pollen research, the text covers topics including advances in understanding pollen tube growth, the use of pollen for gene transfer, and advantages and disadvantages of various pollination systems for production of species limits.
In Vitro Culture of Higher Plants presents an up-to-date and wide- ranging account of the techniques and applications, and has primarily been written in response to practical problems. Special attention has been paid to the educational aspects. Typical methodological aspects are given in the first part: laboratory set-up, composition and preparation of media, sterilization of media and plant material, isolation and (sub)culture, mechanization, the influence of plant and environmental factors on growth and development, the transfer from test-tube to soil, aids to study. The question of why in vitro culture is practised is covered in the second part: embryo culture, germination of orchid seeds, mericloning of orchids, production of disease-free plants, vegetative propagation, somaclonal variation, test-tube fertilization, haploids, genetic manipulation, other applications in phytopathology and plant breeding, secondary metabolites.
Attempting to collect, sort out, comment on and summarize from available literature the relevant information dealing with a specific problem is always a difficult task which necessarily involves sub jective choices and implies a considerable risk of errors and omis sions. The difficulty is increased when, as in the case of incompati bility in angiosperms, the subject to be treated traces its history to preDarwinian times and reflects the total sum of numerous in vestigations dealing with widely different disciplines, such as ge netics, cytology, biochemistry, systematics and physiology, which no single reviewer may pretend to master sufficiently to avoid completely the possibility of misinterpretation. Furthermore, the complexity of the task is further augmented by the fact that the student of incompatibility, confronted as he or she is with still poorly understood phenomena of genetic control and molecular recognition, often tends to be speculative and, in some instances, over-imaginative at the time of fitting research observations and experimental data into appropriate models, schemes and hypo theses. The compensation for such a state of affair is, however, a strong one and lies in the remarkable willingness and readiness of "in compatibilists" to cooperate, and to provide information, ex planations and illustrations to anyone attempting to penetrate into their universe of research and of reflection.
Plant Development and Evolution, the latest release in the Current Topics in Developmental Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on the Evolution of the plant body plan, Lateral root development and its role in evolutionary adaptation, the Development of the vascular system, the Development of the shoot apical meristem and phyllotaxis, the Evolution of leaf diversity, the Evolution of regulatory networks in land plants, The role of programed cell death in plant development, the Development and evolution of inflorescence architecture, the Molecular regulation of flower development, the Pre-meiotic another development, and much more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Current Topics in Developmental Biology series - Updated release includes the latest information on Plant Development and Evolution
Here, for the first time in a single volume, are all the ideas and techniques developed in the last two decades concerning the manipulation of pollen and pollen tubes in plant breeding and biotechnology.