The PCR Revolution
Author: Stephen A. Bustin
Publisher: Cambridge University Press
Published: 2010
Total Pages: 327
ISBN-13: 0521882311
DOWNLOAD EBOOKExamines the latest innovations and the overall impact of PCR on areas of molecular research.
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The Polymerase Chain Reaction
Author: Kary B. Mullis
Publisher: Springer Science & Business Media
Published: 2012-02-02
Total Pages: 464
ISBN-13: 1461202574
DOWNLOAD EBOOKJames D. Watson When, in late March of 1953, Francis Crick and I came to write the first Nature paper describing the double helical structure of the DNA molecule, Francis had wanted to include a lengthy discussion of the genetic implications of a molecule whose struc ture we had divined from a minimum of experimental data and on theoretical argu ments based on physical principles. But I felt that this might be tempting fate, given that we had not yet seen the detailed evidence from King's College. Nevertheless, we reached a compromise and decided to include a sentence that pointed to the biological significance of the molecule's key feature-the complementary pairing of the bases. "It has not escaped our notice," Francis wrote, "that the specific pairing that we have postulated immediately suggests a possible copying mechanism for the genetic material." By May, when we were writing the second Nature paper, I was more confident that the proposed structure was at the very least substantially correct, so that this second paper contains a discussion of molecular self-duplication using templates or molds. We pointed out that, as a consequence of base pairing, a DNA molecule has two chains that are complementary to each other. Each chain could then act ". . . as a template for the formation on itself of a new companion chain, so that eventually we shall have two pairs of chains, where we only had one before" and, moreover, " ...
PCR Technology
Author: Thomas Weissensteiner
Publisher: CRC Press
Published: 2003-11-13
Total Pages: 404
ISBN-13: 1420040650
DOWNLOAD EBOOKA technique used to amplify the number of copies of a specific region of DNA, the polymerase chain reaction (PCR) is at the forefront of the dramatic development of biochemistry. This text provides the tools for developing innovative approaches to using this leading technology. It includes theoretical considerations, discussions, and a selection of
Molecular Biology of the Cell
Author:
Publisher:
Published: 2002
Total Pages: 0
ISBN-13: 9780815332183
DOWNLOAD EBOOKThe $1,000 Genome
Author: Kevin Davies
Publisher: Simon and Schuster
Published: 2010-09-07
Total Pages: 354
ISBN-13: 1416570187
DOWNLOAD EBOOKIn this essential guide to the brave new future, Dr. Kevin Davies, author of Cracking the Genome, reveals the masterful ingenuity that transformed the process of decoding DNA and vividly brings the extraordinary drama of the grand scientific achievement to life. In 2000, President Bill Clinton signaled the completion of the Human Genome Project at a cost in excess of $2 billion. A decade later, the price for any of us to order our own personal genome sequence—a comprehensive map of the 3 billion letters in our DNA—had already dropped to just $1,000. Dozens of men and women—scientists, entrepreneurs, celebrities, and patients—have already been sequenced, pioneering a bold new era of personalized genomic medicine. The $1,000 genome has long been considered the tipping point that would open the floodgates to this revolution. How has this astonishing achievement been accomplished? To research the story of this unfolding revolution, critically acclaimed science writer Kevin Davies traveled to the leading centers and interviewed the entrepreneurs and pioneers in the race to achieve the $1,000 genome. Davies also profiles the future of genomic medicine and thoughtfully explores the many pressing issues raised by the tidal wave of personal genetic information.
PCR Technology
Author: Tania Nolan
Publisher: CRC Press
Published: 2016-10-26
Total Pages: 457
ISBN-13: 9781138198586
DOWNLOAD EBOOK"A technique used to amplify the number of copies of a specific region of DNA, the polymerase chain reaction (PCR) is at the forefront of the dramatic development of biochemistry. This text provides the tools for developing innovative approaches to using this leading technology. It includes theoretical considerations, discussions, and a selection of state-of-the-art techniques for mutation studies, clinical diagnosis, and the detection of food-borne pathogens. This edition also discusses the preparation of PCR experiments, includes examples of analytical PCR divided into qualitative and quantitative applications, and explores preparative methods that address DNA generation for further analysis and in vitro evolution"--Provided by publisher.
Bio-Nanotechnology
Author: Manashi Bagchi
Publisher: John Wiley & Sons
Published: 2012-11-26
Total Pages: 1118
ISBN-13: 1118451937
DOWNLOAD EBOOKBio-nanotechnology is the key functional technology of the 21st century. It is a fusion of biology and nanotechnology based on the principles and chemical pathways of living organisms, and refers to the functional applications of biomolecules in nanotechnology. It encompasses the study, creation, and illumination of the connections between structural molecular biology, nutrition and nanotechnology, since the development of techniques of nanotechnology might be guided by studying the structure and function of the natural nano-molecules found in living cells. Biology offers a window into the most sophisticated collection of functional nanostructures that exists. This book is a comprehensive review of the state of the art in bio-nanotechnology with an emphasis on the diverse applications in food and nutrition sciences, biomedicine, agriculture and other fields. It describes in detail the currently available methods and contains numerous references to the primary literature, making this the perfect “field guide” for scientists who want to explore the fascinating world of bio-nanotechnology. Safety issues regarding these new technologies are examined in detail. The book is divided into nine sections – an introductory section, plus: Nanotechnology in nutrition and medicine Nanotechnology, health and food technology applications Nanotechnology and other versatile applications Nanomaterial manufacturing Applications of microscopy and magnetic resonance in nanotechnology Applications in enhancing bioavailability and controlling pathogens Safety, toxicology and regulatory aspects Future directions of bio-nanotechnology The book will be of interest to a diverse range of readers in industry, research and academia, including biologists, biochemists, food scientists, nutritionists and health professionals.
Preventable
Author: Andy Slavitt
Publisher: St. Martin's Press
Published: 2021-06-15
Total Pages: 199
ISBN-13: 1250770173
DOWNLOAD EBOOK* NATIONAL BESTSELLER * “Painfully good. The book could have been called, ‘Outrageous.’ The story Andy Slavitt tells is not just about Trump’s monumental failures but also about the deeper ones that started long before, with our health system, our politics, and more.” --Atul Gawande, author of Being Mortal The definitive, behind-the-scenes look at the U.S. Coronavirus crisis from one of the most recognizable and influential voices in healthcare From former Biden Senior Advisor Andy Slavitt, Preventable is the definitive inside account of the United States' failed response to the Coronavirus pandemic. Slavitt chronicles what he saw and how much could have been prevented -- an unflinching investigation of the cultural, political, and economic drivers that led to unnecessary loss of life. With unparalleled access to the key players throughout the government on both sides of the aisle, the principal public figures, as well as the people working on the frontline involved in fighting the virus, Slavitt brings you into the room as fateful decisions are made and focuses on the people at the center of the political system, health care system, patients, and caregivers. The story that emerges is one of a country in which -- despite the heroics of many -- bad leadership, political and cultural fractures, and an unwillingness to sustain sacrifice light a fuse that is difficult to extinguish. Written in the tradition of The Big Short, Preventable continues Andy Slavitt’s important work of addressing the uncomfortable realities that brought America to this place. And, he puts forth the solutions that will prevent us from being here again, ensuring a better, stronger country for everyone.
Forensic DNA Profiling Protocols
Author: Patrick J. Lincoln
Publisher: Springer Science & Business Media
Published: 1998-01-22
Total Pages: 617
ISBN-13: 0896034437
DOWNLOAD EBOOKThis state-of-the-art collection of easily reproducible methods includes all of the major techniques of DNA analysis currently used in forensic identity testing. The methods include the recovery of DNA from a large range of sample types, analysis of DNA as single and multi-locus VNTR probes, PCR amplification of STR and other loci, and mitochondrial sequencing. The expert scientists writing here -- many from laboratories around the world -- also discuss how to interpret the results in cases of unknown identity and disputed parentage.-- Covers all steps from extraction of human DNA through to analysis and interpretation-- Takes advantage of new methodologies such as capillary electrophoresis-- Clear step-by-step instructions ensure unfailing reproducibility.
Gene Quantification
Author: Francois Ferre
Publisher: Springer Science & Business Media
Published: 2012-12-06
Total Pages: 379
ISBN-13: 1461241642
DOWNLOAD EBOOKGeneticists and molecular biologists have been interested in quantifying genes and their products for many years and for various reasons (Bishop, 1974). Early molecular methods were based on molecular hybridization, and were devised shortly after Marmur and Doty (1961) first showed that denaturation of the double helix could be reversed - that the process of molecular reassociation was exquisitely sequence dependent. Gillespie and Spiegelman (1965) developed a way of using the method to titrate the number of copies of a probe within a target sequence in which the target sequence was fixed to a membrane support prior to hybridization with the probe - typically a RNA. Thus, this was a precursor to many of the methods still in use, and indeed under development, today. Early examples of the application of these methods included the measurement of the copy numbers in gene families such as the ribosomal genes and the immunoglo bulin family. Amplification of genes in tumors and in response to drug treatment was discovered by this method. In the same period, methods were invented for estimating gene num bers based on the kinetics of the reassociation process - the so-called Cot analysis. This method, which exploits the dependence of the rate of reassociation on the concentration of the two strands, revealed the presence of repeated sequences in the DNA of higher eukaryotes (Britten and Kohne, 1968). An adaptation to RNA, Rot analysis (Melli and Bishop, 1969), was used to measure the abundance of RNAs in a mixed population.
