Microscopic Images of Human Sperm, Oocytes and Embryos for Assisted Reproductive Technology Including Embryonic Stem Cells

Microscopic Images of Human Sperm, Oocytes and Embryos for Assisted Reproductive Technology Including Embryonic Stem Cells

Author: Professor Arunachalam Henry Sathananthan

Publisher: Professor Arunachalam Henry Sathananthan

Published: 2014-12-29

Total Pages: 434

ISBN-13: 0994216505

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This Epub incorporates 37 years of embodied research at Monash & La Trobe Universities, Melbourne, by the author with his colleagues in Australia and overseas (England, Singapore, India, Sri Lanka, Italy & Japan).It is a multi-author, visual presentation of the microscopic structure of human oocytes, sperm and embryos in conjunction with clinical IVF and ART and includes embryonic stem cells, a logical extension of ART.Most of the images were taken with electron microscopes (TEM & SEM), light and phase microscopes (LM) and fluorescent microscopes (FM) with diagrams.Both published micrographs (from our atlases and journals) and also original unpublished micrographs are presented. The printed atlases were scanned and new material was provided on PPT, PDF & YouTube for EPub.The aim of this EPub is to make our research available online for clinicians, scientists & students and posterity. A. Henry Sathananthan (Adjunct Professor, Anatomy & Developmental Biology,Medical Faculty, Monash University, Australia)


Egg and Sperm Race

Egg and Sperm Race

Author: Matthew Cobb

Publisher:

Published: 2006

Total Pages: 362

ISBN-13:

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Where do we come from? Where do animals come from? For thousands of years we really had no clue how living things were created -- great thinkers like Aristotle and Plato had attempted to explain what became known as the problem of 'generation', but neither really had the tools or the insight to solve the mystery. The result was a wealth of weird and wonderful ideas about the components necessary to create new life -- blood, 'vapours', strange pulses in the air. Nor did people make intuitive leaps that now seem self-evident: it was widely accepted that animals could breed different species, for example; the notion that two sheep can only make another sheep is a surprisingly modern idea.But all this confusion changed in a flurry of discovery in the mid-seventeenth century. In just a decade, a group of young scientists in Europe, all known to each other and in competition with each other, established the existence first of the human egg and then of the human sperm. At last, the building blocks were in place -- although, in one of the great ironies of science, it would be another 150 years before someone worked out how fertilisation actually took place.Focusing on the personalities and rivalries of this extraordinary period, Matthew Cobb has shed new light not just on an under-reported story of science but on our very nature -- what makes us, and how little we still know about one of the greatest miracles of Nature.


Atlas of Human Sperm Morphology Evaluation

Atlas of Human Sperm Morphology Evaluation

Author: Thinus F. Kruger

Publisher: CRC Press

Published: 2004-07-26

Total Pages: 86

ISBN-13: 9781842142776

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Sperm morphology evaluation by strict criteria is the gold-standard used by IVF and andrology units around the world. It is based on an approach to sperm morphology evaluation that utilizes optimal preparation methods, evaluation procedures, and criteria for a morphological normal spermatozoon based on biological evidence. Featuring fresh perspectives from the founders of the Tygerberg strict criteria, Atlas of Human Sperm Morphology Evaluation provides state-of-the-art information on crucial aspects of male factor infertility. The atlas consists of an introductory teaching manual that gives readers the ability to train and test their diagnostic capabilities and enables laboratories to implement quality control measures. Together with a team of internationally renowned experts in the field of human reproduction, the editors explore every avenue you need to know to successfully evaluate and manage male factor infertility. An accompanying interactive CD-ROM training program, STRICT 1-2-3® is available from the editors. STRICT 1-2-3® has been developed alongside the atlas to provide not only a complete theoretical background on sperm morphology, but also self-training facilities. Laboratory directors and technicians will now be able to set up their own internal quality control program for sperm morphology evaluation.


A Comparative Overview of Mammalian Fertilization

A Comparative Overview of Mammalian Fertilization

Author: Bonnie S. Dunbar

Publisher: Springer Science & Business Media

Published: 2013-06-29

Total Pages: 458

ISBN-13: 1475789823

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In 1964, the Fertilization and Gamete Physiology Research Training Program (FERGAP) was established at the Marine Biological Laboratories, Woods Hole, Massachusetts. Over the course of the next 12 years, under the directorship of Dr. Charles B. Metz, FERGAP brought together, trained, and inspired a generation of students in reproductive biology from all over the world. As students of C. B. Metz and as FERGAP trainees, we would like to dedicate this collected work on comparative mammalian fertilization to our teacher and mentor, Dr. Charles B. Metz. Like a number of authors contributing to this volume, we have been struck by the significant impact that C. B. Metz and FERGAP had on the development of students of reproductive biology. Applying both the classical and molecular techniques of cell biology and immunology to problems of gamete biology, Dr. Metz emphasized a comparative and analytical approach that was reflected in his own research on fertilization in Paramecia, sea urchins, frogs, and mammals. It is hoped that this volume will serve to stimulate students to discover the myriad of fascinating research problems in gamete and reproductive biology. Bonnie S. Dunbar Michael G. O'Rand Houston, Texas Chapel Hill, North Carolina ix Contents Part I COMPARATIVE OVERVIEW OF MAMMALIAN GAMETES The Coevolution of Mammalian Gametes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 J. Michael Bedford I. Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 2. Gamete Design . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 2.1. Monotremes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 . . . . . . . . . . . . . . 2.2. Marsupials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 . . . . . . . . . . . . . . . 2.3. Eutherians . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 3. Gamete Maturation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 . . . . . . . . . . . . . . . 3 .1. Oocyte Maturation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 . . . . . . . . . . . . . 3.2. Sperm Maturation in the Male . . . . . . . . . . . . . . . . . . . . . . . 15 . . . . . . . . . .