Quantitative Imaging in Cell Biology
Quantitative Imaging in Cell Biology

Author:

Publisher: Academic Press

Published: 2014-06-25

Total Pages: 609

ISBN-13: 0124202012

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This new volume, number 123, of Methods in Cell Biology looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications. The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detector impact the quality of quantitative data, and include step-by-step protocols for capturing and analyzing quantitative images. Common quantitative applications, including co-localization, ratiometric imaging, and counting molecules, are covered in detail. Practical chapters cover topics critical to getting the most out of your imaging system, from microscope maintenance to creating standardized samples for measuring resolution. Later chapters cover recent advances in quantitative imaging techniques, including super-resolution and light sheet microscopy. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material

Under the Microscope
Under the Microscope

Author: William J. Croft

Publisher: World Scientific

Published: 2006

Total Pages: 153

ISBN-13: 9810237812

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This is a brief history of the development of microscopy, from the use of beads and water droplets in ancient Greece, through the simple magnifying glass, to the modern compound microscope. The technology and optical theory are developed in a straightforward manner, and this leads to a description and explanation of the most modern technologies in electron microscopy, and scanning electron microscopy as well as the new scanning probe microscopies. A series of very interesting applications of the various microscopic techniques are described. The most recent pioneering techniques in near field and confocal optical microscope technologies are described and evaluated for their future importance.

Focus On Multidimensional Microscopy (In 2 Vols) - Volume 1
Focus On Multidimensional Microscopy (In 2 Vols) - Volume 1

Author: Ping-chin Cheng

Publisher: World Scientific

Published: 1999-12-28

Total Pages: 320

ISBN-13: 9814497711

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This book covers various aspects of modern microscopy, with emphasis on multidimensional (three-dimensional and higher) and multimodality microscopy. The topics discussed include multiphoton fluorescent microscopy, confocal microscopy, x-ray microscopy and microtomography, electron microscopy, probe microscopy and multidimensional image processing for microscopy. In addition, there are chapters demonstrating typical microscopical applications, both biological and material.

A Practical Guide to Ore Microscopy—Volume 1
A Practical Guide to Ore Microscopy—Volume 1

Author: Ricardo Castroviejo

Publisher: Springer Nature

Published: 2023-07-12

Total Pages: 951

ISBN-13: 3031126548

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This book offers a guide to the microscopic study of metallic ores with reflected light. It combines a rigorous approach with an attractive and easy-to-follow format, using high-quality calibrated photomicrographs to illustrate the use of color for ore identification. The ore identification methodology is updated with systematic color analysis and the application of new multispectral reflectance datasets, which offer an efficient tool for automated ore characterization. In addition, the first volume of this two-part work discusses the essential gangue minerals. Readers will gain familiarity with the method as they follow its application to over 200 selected minerals, comprising the most important ore (≈150) and gangue (≈50) minerals, which are described in the text. Each entry includes an explanatory text with corresponding color photomicrographs for each of the most common microscope settings, preceded by a table summarizing the ore’s main properties, and followed by spectral information in the visible and near-infrared ranges (specular reflectance values from 370 to 1000 nm). Some uncommon, strategic ores, e.g. columbotantalite (“coltan”) and monazite, receive particular attention or are described for the first time with reflected light. Lastly, the book presents a learning strategy for beginners and students. The approach is essentially practical, focusing on the development of observation skills, including self-checking through proposed practical tasks. In addition, the traditional use of determinative tables is critically reviewed and updated. This book is part of a two-volume work. The second volume focuses on intergrowths/textural analysis and interpretation, as well as computer-vision-based automation and applications to ore processing/geometallurgy. The intended audience includes professionals and engineers dealing with mineral resources, as well as postgraduate students. The book also provides lifelong learning support for freelancers and a valuable reference resource for practical university teaching.

Under the Microscope
Under the Microscope

Author: Jeremy Burgess

Publisher: CUP Archive

Published: 1990-07-12

Total Pages: 212

ISBN-13: 9780521399401

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"A celebration of the hidden beauty & variety of microscopic imagery."--Back cover.

The Microscope Past and Present
The Microscope Past and Present

Author: S. Bradbury

Publisher: Elsevier

Published: 2013-10-22

Total Pages: 283

ISBN-13: 1483138976

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The Microscope: Past and Present discusses the best methods of using microscope. This book presents a concise account of the development of this valuable tool, which is used at some time or another by almost every scientist. Comprised of seven chapters, this book begins with an overview of the factors that stimulated the rapid progress in microscopy. This text then describes the immense importance of the work of Robert Hooke in the development of optical devices for assisting the human eye. Other chapters consider the different types of compound microscopes and describe the single-lens or simple microscopes. This book discusses as well the development of the mechanical aspect of the microscope that resulted in a vast increase in the professional use of the microscope. The final chapter deals with the development of electron microscopes. This book is a valuable resource for medical students, scientists, and specialist workers in all fields of industry.

Surface and Interface Science, Volumes 1 and 2
Surface and Interface Science, Volumes 1 and 2

Author: Klaus Wandelt

Publisher: John Wiley & Sons

Published: 2012-04-16

Total Pages: 1010

ISBN-13: 3527411569

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Covering interface science from a novel surface science perspective, this unique handbook offers a comprehensive overview of this burgeoning field. Eight topical volumes cover basic concepts and methods, elemental and composite surfaces, solid-gas, solid-liquid and inorganic biological interfaces, as well as applications of surface science in nanotechnology, materials science and molecular electronics. With its broad scope and clear structure, it is ideal as a reference for scientists in the field, as well as an introduction for newcomers.

Scanning Electron Microscopy and X-Ray Microanalysis
Scanning Electron Microscopy and X-Ray Microanalysis

Author: Joseph Goldstein

Publisher: Springer Science & Business Media

Published: 2013-11-11

Total Pages: 679

ISBN-13: 1461332737

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This book has evolved by processes of selection and expansion from its predecessor, Practical Scanning Electron Microscopy (PSEM), published by Plenum Press in 1975. The interaction of the authors with students at the Short Course on Scanning Electron Microscopy and X-Ray Microanalysis held annually at Lehigh University has helped greatly in developing this textbook. The material has been chosen to provide a student with a general introduction to the techniques of scanning electron microscopy and x-ray microanalysis suitable for application in such fields as biology, geology, solid state physics, and materials science. Following the format of PSEM, this book gives the student a basic knowledge of (1) the user-controlled functions of the electron optics of the scanning electron microscope and electron microprobe, (2) the characteristics of electron-beam-sample inter actions, (3) image formation and interpretation, (4) x-ray spectrometry, and (5) quantitative x-ray microanalysis. Each of these topics has been updated and in most cases expanded over the material presented in PSEM in order to give the reader sufficient coverage to understand these topics and apply the information in the laboratory. Throughout the text, we have attempted to emphasize practical aspects of the techniques, describing those instru ment parameters which the microscopist can and must manipulate to obtain optimum information from the specimen. Certain areas in particular have been expanded in response to their increasing importance in the SEM field. Thus energy-dispersive x-ray spectrometry, which has undergone a tremendous surge in growth, is treated in substantial detail.