The Blotting Book
Author: E.F. Benson
Publisher: BoD – Books on Demand
Published: 2019-09-25
Total Pages: 97
ISBN-13: 3734093287
DOWNLOAD EBOOKReproduction of the original: The Blotting Book by E.F. Benson
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Author: E.F. Benson
Publisher: BoD – Books on Demand
Published: 2019-09-25
Total Pages: 97
ISBN-13: 3734093287
DOWNLOAD EBOOKReproduction of the original: The Blotting Book by E.F. Benson
Author: Edward Frederic Benson
Publisher:
Published: 1908
Total Pages: 266
ISBN-13:
DOWNLOAD EBOOKAuthor: Martin Edwards
Publisher: Sourcebooks, Inc.
Published: 2017-08-01
Total Pages: 333
ISBN-13: 1464207240
DOWNLOAD EBOOK2018 ALA Book Club October Pick, Things that Go Bump: Paranormal Mysteries David Randall's perfect family life came derailed when his little daughter Lindsey died in a car crash. Thrown out by his second wife and wanting to leave a dead-end detective agency to start his own, he reluctantly accepts his psychic friend Camden's invitation to stay in Camden's boarding house in Parkland, North Carolina. Meanwhile, working the case of the murder of Albert Bennett, Randall's only clue is a notebook filled with odd musical notation. When another client, Melanie Gentry, hires him to prove her great-grandmother was murdered by her lover, composer John Burrows Ashford, over authorship of "Patchwork Melodies," Randall sets out to find a connection to Bennett's murder, as well as to the murder of a Smithsonian director, who was preparing a new PBS documentary on early American music. Randall's investigations lead him to another notebook, where he finds not only "Two Hearts Singing," Ashford's most famous song, but a valuable early copy of Stephen Foster's "Oh! Susanna," hidden in the cover. But things become more complicated when Ashford's spirit parks itself in Cam...and refuses to leave until Randall proves Ashford's innocence.
Author: Biji T. Kurien
Publisher: Springer Nature
Published: 2021-07-24
Total Pages: 467
ISBN-13: 3030706842
DOWNLOAD EBOOKThis book fills the need for a simplified text covering western blotting protocols aimed not just at high school and college students, but the researcher with little to no experience in these techniques. It provides the principles, basic methodology, and tips and tricks to avoiding the common pitfalls of western blotting. The book also introduces simple protocols that can transform western blotting into a fun method, such as sending secret messages on membranes or using nitrocellulose membrane as a canvas for art. In addition to the techniques, this book also covers the history of western blotting, which originated from the development of the blotting of DNA. It then delves into the importance of protein blotting, brought to the fore by the fact that the procedure has been evolving constantly since its inception in 1979, and the fact that the scientific community is faced with a multitude of ways and means of transferring proteins to membranes..
Author: Biji T. Kurien
Publisher: Humana
Published: 2015-06-05
Total Pages: 0
ISBN-13: 9781493926930
DOWNLOAD EBOOKThis volume covers past and present western blot techniques, such as diffusion blotting, slice blotting, blotting of high and low molecular weight proteins, single cell blotting and automated blotting. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Western Blotting: Methods and Protocols will serve as an invaluable reference for those interested in further study into this fascinating field.
Author: Ayaz Najafov
Publisher: Academic Press
Published: 2017-07-07
Total Pages: 101
ISBN-13: 0128135387
DOWNLOAD EBOOKWestern Blotting Guru provides researchers in molecular biology with a handy reference for approaching and solving challenging problems associated with immunoblotting setup and optimization. As a laboratory guide, it emphasizes the technical aspects of efficiently employing immunoblotting as a tool in molecular biology laboratories. The book covers the basic science underlying immunoblotting and detailed description of the method parameters, followed by good benchtop practices, tips and tricks for obtaining high-quality data and a detailed troubleshooting guide addressing a variety of problem types. - Provides a benchtop reference that every molecular biologists will use to design, optimize, troubleshoot and analyze their immunoblotting experiments - Contains unique good practices and tips that are indispensable for the beginner and expert alike - Features special cases with applications of immunoblotting optimization - Includes detailed appendices with tables, figures and key protocols - Provides troubleshooting tips for various types of modifications of standard protocols - Organized as a systematic, concentrated resource to save time when addressing an immunoblotting problem
Author: Darling
Publisher: BIOS Scientific Publishers
Published: 1994-11-01
Total Pages: 0
ISBN-13: 9780199634460
DOWNLOAD EBOOKDiscusses very widely-used techniques. Aimed specifically at the newcomer. Provides detailed explanations unavailable elsewhere. Points out pitfalls and provides solutions. Invaluable help for experienced laboratories training newcomers. Foreward wri
Author: Larry J. Kricka
Publisher: Elsevier
Published: 1995-06-01
Total Pages: 539
ISBN-13: 0080537669
DOWNLOAD EBOOKSince the publication of Nonistopic DNA Probe Techniques in 1992, the move away from radioactive materials for research and diagnostics has continued. This is due in part to public awareness of the hazards of radioactive waste and laws making radioactive disposal more difficult and costly and to improvement in both the sensitivity and convenience of nonisotopic techniques. Several new nonisotopic techniques have been developed and substantial improvements made to existing nonisotopic methods since 1992, and these are now included in Nonisotopic Probing, Blotting, and Sequencing. Nonisotopic Probing, Blotting, and Sequencing is an updated, expanded edition of the bestseller, Nonisotopic DNA Probe Techniques. It has been thoroughly revised to include the latest improvements in nonisotopic tagging techniques for macromolecules. Like its predecessor, it enables researchers to select the best nonisotopic method for their needs and maximize success by following its straightforward protocols. - Provides strategies and detailed procedures for labeling, blotting, and probing specific nucleic acid sequences and, with this edition, protein molecules - Gives protocols for nonisotopic DNA sequencing - new in this edition - Gives extensive, practical information - Presents background information for each method - Provides expert accounts from the inventor or developer of each method - Contains seven entirely new chapters - Covers all major types of nonisotopic procedures for labeling and detection
Author: Biji T. Kurien
Publisher: Humana Press
Published: 2009-05-15
Total Pages: 588
ISBN-13: 9781934115732
DOWNLOAD EBOOKOver the past thirty years, the development of the Western blot has revolutionized the fields of biomedical research and medical diagnostics. In "Protein Blotting and Detection: Methods and Protocols", expert researchers present numerous techniques based on the Western blot, providing detailed, readily reproducible methods, tips, and alternatives directly and easily transferable to the laboratory setting. Chapters offer a large number of variations on the theme of protein transfer to solid support followed by detection, presenting both adaptations of traditional techniques as well as completely original methods of protein blotting. Composed in the highly successful Methods in Molecular BiologyTM series format, each chapter contains a brief introduction, a list of necessary materials, step-by-step methods, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Innovative and highly practical, Protein Blotting and Detection: Methods and Protocols is an essential, hands-on guide for all investigators who hope to bring these cutting-edge procedures home to their laboratories.
Author: James Montgomery Boice
Publisher: Crossway
Published: 2009-04-01
Total Pages: 242
ISBN-13: 1433517353
DOWNLOAD EBOOKThere is no question that we live in an age of weak theology and casual Christianity. We have substituted intuition for truth, feeling for belief and immediate gratification for enduring hope. Evangelicalism desperately needs to return to the doctrines that once before reformed the world: radical depravity, unconditional election, particular redemption, efficacious grace and persevering grace. James Boice and Philip Ryken not only provide a compelling exposition on these doctrines of grace, but also look briefly at their historical impact. The authors leave no doubt that the church suffers when these foundational truths are neglected and that she must return to a Christianity that is practical-minded, kind-hearted, and most importantly, biblically based.