Superantigen Protocols
Superantigen Protocols

Author: Teresa Krakauer

Publisher: Springer Science & Business Media

Published: 2008-02-05

Total Pages: 259

ISBN-13: 1592593674

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Leading researchers in the biological, chemical, and physical investigation of superantigens describe in step-by-step detail their best experimental techniques to assess the physical characteristics and biological effects of superantigens. Their protocols range from those for investigating the interactions of superantigens with cellular receptors to those for the analysis of their immunological and biological effects, including methods for using BIOcore to determine binding kinetics and establishing various lymphocyte cell culture systems. There are also accounts of such methods as the RNase protection assay, cytokine ELISA, FACS analysis, and cytokine production at the single cell level..

Superantigens
Superantigens

Author: Amanda J. Brosnahan

Publisher:

Published: 2016

Total Pages: 228

ISBN-13: 9781493933440

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This volume combines protocols that encompass the true variety of investigation done on superantigens in the fields of microbiology, immunology, molecular biology, biochemistry, and cellular biology, with a strong focus on disease models utilized to determine the role of superantigens in human disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Superantigens: Methods and Protocols contains a detailed and wide breadth of subject coverage that any scientist, clinician, or industry professional interested in this field will find valuable.

Superantigen Protocols. Methods in Molecular Biology
Superantigen Protocols. Methods in Molecular Biology

Author: Teresa Krakauer

Publisher:

Published: 2003

Total Pages:

ISBN-13:

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Leading researchers in the biological, chemical, and physical investigation of superantigens describe in step-by-step detail their best experimental techniques to assess the physical characteristics and biological effects of superantigens. Their protocols range from those for investigating the interactions of superantigens with cellular receptors to those for the analysis of their immunological and biological effects, including methods for using BIOcore to determine binding kinetics and establishing various lymphocyte cell culture systems. There are also accounts of such methods as the RNase protection assay, cytokine ELISA, FACS analysis, and cytokine production at the single cell level.

Superantigens
Superantigens

Author: Bernhard Fleischer

Publisher: Springer Science & Business Media

Published: 2013-03-09

Total Pages: 144

ISBN-13: 3642509983

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This volume of Current Topics in Microbiology and Immunology is concerned with a class of molecules that are the most potent polyclonal stimulators of T lymphocytes of several species. These molecules have been named "superantigens" because they use a mechanism of T cell stimulation closely mimicking MHC-restricted recognition of specific antigen: they act on variable parts of T cell antigen receptors and are presented by MHC class II molecules. Prototypes of these molecules are the pyrogenic exotoxins produced by S. aureus and S. pyogenes, of which the staphylococcal enterotoxins and the toxic shock syndrome toxin are the best known. Superantigens also occur endogen ously in mice, most notably the enigmatic Mis determinants, that have withstood characterization for nearly 20 years. Only very recently was it found that Mis is probably encoded by endogenous retroviruses. The list of candidates that are implicated as being superantigens is growing. In many cases, however, the proof that a given molecule indeed falls into this category is still missing.

Superantigens
Superantigens

Author: Leung

Publisher: CRC Press

Published: 1997-02-20

Total Pages: 626

ISBN-13: 9780824798130

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This up-to-date sourcebook covers viral and bacterial superantigens (SAgs) from molecular structure and immunological processes to pathology and treatment of superantigen-mediated human diseases. Discusses diseases beyond Toxic Shock Syndrome, such as autoimmune and inflammatory skin conditions, as well as the role of superantigens in other infectious diseases. Illustrated with molecular structures of superantigens.

Superantigens and Superallergens
Superantigens and Superallergens

Author: Gianni Marone

Publisher: Karger Medical and Scientific Publishers

Published: 2007-01-01

Total Pages: 259

ISBN-13: 3805582668

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Superantigens constitute a growing family of bacterial and viral proteins that share the capacity of inducing massive activation of the immune system. This volume contains contributions from leaders in the field of classical T cell superantigens and sheds light on relevant B cell superantigens at the molecular and biochemical levels. This is the first volume to put together basic and clinical aspects of the classical T cell superantigens, B cell superantigens and superallergens. It will therefore be indispensable reading to basic and clinical immunologists, microbiologists, virologists and specialists in allergic and immune disorders.

Superantigens
Superantigens

Author: Brigitte T. Huber

Publisher: CSHL Press

Published: 1993

Total Pages: 198

ISBN-13: 9780879693985

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Superantigens are microbial products that defy the conventional notion of lymphocyte activation to produce an unusually vigorous, genetically unrestricted response among many subsets of antigen-specific T cells. Bacterial toxins and certain viral gene products behave as superantigens, deleting T cells in vivo and stimulating their proliferation in vitro. These observations raise many fascinating questions about the nature of T-cell activation and microbial pathogenesis. This volume is an up-to-date, wide-ranging examination of the function and significance of superantigens in virology, biochemistry, and cell and stuctural biology as well as immunology. The book will interest graduate students and postdoctoral fellows in a wide range of biomedical sciences.

Superantigen Protocols
Superantigen Protocols

Author: Teresa Krakauer

Publisher: Humana

Published: 2002-10-23

Total Pages: 259

ISBN-13: 9780896039841

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Leading researchers in the biological, chemical, and physical investigation of superantigens describe in step-by-step detail their best experimental techniques to assess the physical characteristics and biological effects of superantigens. Their protocols range from those for investigating the interactions of superantigens with cellular receptors to those for the analysis of their immunological and biological effects, including methods for using BIOcore to determine binding kinetics and establishing various lymphocyte cell culture systems. There are also accounts of such methods as the RNase protection assay, cytokine ELISA, FACS analysis, and cytokine production at the single cell level.

MHC Protocols
MHC Protocols

Author: Stephen H. Powis

Publisher: Springer Science & Business Media

Published: 2008-02-05

Total Pages: 343

ISBN-13: 1592592910

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The aim of MHC Protocols is to document protocols that can be used for the analysis of genetic variation within the human major histocompatibility complex (MHC; HLA region). The human MHC encompasses approximately 4 million base pairs on the short arm of chromosome 6 at cytogenetic location 6p21. 3. The region is divided into three subregions. The telomeric class I region contains the genes that encode the HLA class I molecules HLA-A, -B, and -C. The centromeric class II region contains the genes encoding the HLA class II molecules HLA-DR, -DQ, and -DP. In between is the class III region, originally identified because it contains genes encoding components of the complement pathway. The entire human MHC has recently been sequenced (1) and each subregion is now known to contain many other genes, a number of which have immunological functions. The study of polymorphism within the MHC is well established, because the region contains the highly polymorphic HLA genes. HLA polymorphism has been used extensively in solid organ and bone marrow transplantation to match donors and recipients. As a result, large numbers of HLA alleles have been identified, a process that has been further driven by recent interest in HLA gene diversity in ethnic populations. The extreme genetic variation in HLA genes is believed to have been driven by the evolutionary response to infectious agents, but relatively few studies have analyzed associations between HLA genetic variation and infectious disease, which has been difficult to demonstrate.