Studies on genetic relationships among six varieties of jackfruit in Kerala employing the "matK" gene using PCR technique and RFLP markers

Studies on genetic relationships among six varieties of jackfruit in Kerala employing the

Author: Prem Jose Vazhacharickal

Publisher: GRIN Verlag

Published: 2017-01-16

Total Pages: 86

ISBN-13: 3668380600

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Scientific Study from the year 2017 in the subject Biology - Genetics / Gene Technology, Mar Augusthinose College, language: English, abstract: Artocarpus heterophyllus belong to the Moraceae family and are abundant in Western Ghats. The fruit provide two MJ per kg/wet weight of ripe perianth and contain high levels of carbohydrates, protein, starch, calcium and vitamins. Jackfruit has diverse medicinal uses especially for anti-oxidant, anti-inflammatory, antimicrobial, anti-cancer and anti-fungal activity. MatK (maturase K) genes are fast evolving, highly variant regions of plant chloroplast DNA that can serve as potential biomarkers for DNA coding and also in generating primers for plants with identification of unique motif regions. Advances in the genetic markers such as RFLP and PCR based methods are more reliable for identification of genetic diversity than morphological markers, although each technique has advantages and limitations. The objective of this research work was to estimate the level of genetic diversity and to assess genetic relationships among six varieties of jackfruit using ‘matK gene’ based on PCR technique and RFLP markers. The partial sequence of the ‘matK’ gene of six different Artocarpus varities was used in the analysis. The size of amplified products was approximately 700 bp. After sequencing and sequence editing, sequence information on a 674 bp region was finally obtained for analysis. The alignment of sequences revealed two haplotypes out of 674 sites. The nucleotide frequencies are 30.00% (A), 37.69% (T/U), 17.93% (C), and 14.39% (G). Being one of the underutilized fruits in India, Artocarpus heterophyllus Lam. has promising leads to further scientific researches and livelihood strategies. The study of matK gene using PCR and RFLP seems to a promising tool in establishing genetic diversity among jackfruit varities. The tree, indigenous to the Western Ghats, is an important source of nutritious food during summer season. Encouragements should be made to the marketing as well as value added food products from this underutilized fruit tree.


Morphological characterization of sweet potato (Ipomoea batatas Lam.) varieties in Kerala: an overview

Morphological characterization of sweet potato (Ipomoea batatas Lam.) varieties in Kerala: an overview

Author: Prem Jose Vazhacharickal

Publisher: Amazon

Published: 2017-11-21

Total Pages: 57

ISBN-13: 1973352524

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Sweet potato (Ipomoea batatas Lam.) is the seventh most important food crop among the root and tuber crops grown in the world which is widely cultivated in tropic, sub tropic and warmer temperate regions. A wide variation exists among sweet potato varieties. The molecular markers Is an important genetic diversity analysis tool for enhancing duplicates identification. The samples were collected based on an elaborative iterative survey as well as traditional knowledge from local people. Thirteen different varieties Ipomoea batatas Lam. varieties (Kuravanpady 1, Kuravanpady 2, Jellipara 1, Jellipara 2, Kottathara, Kadanadu, Kanaka, Aruna, Kanjagad, Cherukizhangu 1, Cherukizhangu 2, Thodupuzha. Palakkad) were used for morphological characterization. Morphological data were collected 60 days after planting based on the descriptors for sweet potato (IBPGR, 1991) in all the 13 samples. The variable scored were twining, plant type, ground cover, vine inter node, vine pigmentation, vine tip pubescence, mature leaf shape, mature leaf size, abaxial leaf vein pigmentation, foliage colour, petiole length, petiole pigmentation, storage root and skin colour. Molecular characterization could be more effectively used in screening varieties among sweet potatoes if coupled with other molecular markers especially ISSR, matK and rbcL markers.


Jackfruit

Jackfruit

Author: Sisir Mitra

Publisher: CABI

Published: 2023-01-06

Total Pages: 309

ISBN-13: 1800622295

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Jackfruit (Artocarpus heterophyllus), is a species of tree in the fig, mulberry, and breadfruit family (Moraceae) and is widely esteemed in tropical Asia. The jackfruit tree is well suited to tropical lowlands, and is widely cultivated throughout South and South-East Asia. It is also grown to a limited extent in Australia, USA, East Africa, as well as in Brazil, Mexico and the Caribbean. Producing giant fruits which can reach up to 80kg in size, jackfruit is the largest tree-borne fruit in the world. It is highly versatile, providing food, timber, fuel in addition to medicinal and industrial products. The ripe fruit is sweet and is more often used for desserts. Canned green jackfruit has a mild taste and meat-like texture that lends itself to being called a 'vegetable meat'. Hence, it is growing in popularity due to its use as a vegan meat alternative. Containing information on jackfruit production technology, postharvest management and processing, this is a valuable resource for researchers in horticulture, plant science, and those interested in sustainable food systems.


Breeding and Biotechnology of Tea and its Wild Species

Breeding and Biotechnology of Tea and its Wild Species

Author: Tapan Kumar Mondal

Publisher: Springer Science & Business Media

Published: 2014-02-10

Total Pages: 179

ISBN-13: 8132217047

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Tea is an important non-alcoholic beverage plant of the world. Cultivation of tea is very important as it earns revenue for the tea growing nations especially the developing countries such as India. Although conventional breeding is well-established and has contributed significantly for varietal improvement of this plant and other Camellia species with ornamental value, yet applications of biotechnology are required to intervene some of the issues where conventional breeding is restricted particularly for woody plants such as tea. It is note-worthy to mention that some amounts of biotechnology works in several facets of tea and its wild species have also been done. In the present book, a state-of-the-art on various aspects of breeding and biotechnology has been complied in eight chapters. They are: i) Origin and descriptions of health benefits as well as morphological classification as first chapter, ii) Breeding and cytogenetics that comprise with various conventional approaches of varietal improvement of tea along with their genetic resources, iii) Micropropagation which deals with in-depth study of clonal propagation, iv) Somatic embryogenesis along with alternative techniques such as suspension culture, cry-preservation etc. v) Molecular breeding that deals with application of various DNA-based markers, linkage map etc., vi) Genetic transformation and associated factors, vii) Stress physiology complied with various works done in tea along with its wild relatives on abiotic as well as biotic stress, and viii) Functional genomics that describe the various works of molecular cloning and characterizations, differential gene expression, high-throughput sequencing, bioinformatics etc. Importantly, the author has made exclusive tables in most of the chapters that include the summary of the works in particular topic. In a nutshell, the book compiles the work already been done, identifies the problems, analyzes the gaps on breeding and biotechnological works of tea as well as its wild species and discusses the future scope as conclusion. Every effort has been made to include all the published works till June 2013. The book will be a useful resource for post-graduate, doctoral as well post-doctoral students working on tea as well as other woody plants. This will also be useful for the scientists working in the areas of life sciences, genomics, biotechnology and molecular biology.


Fruit Breeding

Fruit Breeding

Author: Dinesh, M.R.

Publisher: New India Publishing Agency

Published: 2015-01-15

Total Pages: 6

ISBN-13: 9383305517

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Fruit Breeding is an art and science demanding prolonged time, space and intrinsic qualities. Seed propagation leads to heterogeneity and asexual propagation of elite clones’ leads to newer and novel types. There are temperate, sub-tropical and tropical fruits differing in photo and thermo periodic requirements. There are also parthenocarpic and apomictic fruit trees demanding special breeding methods for improvement. Aroma, taste and flavor are unique to fruits unlike other horticultural crops like vegetables, spices, plantation crops and tubers. The book unravels history of fruit breeding, biodiversity, challenges approaches and prospects, apomixes, mutation breeding, role of insects in fruit production, molecular markers, statistical techniques, rootstock breeding, breeding of mango, citrus, banana, papaya, guava, sapota, jackfruit, sugarapple/sweetsop Indian jujube and jamun. The 20 s in the book authored by 15 scientists from six Research Institutes and Universities expose readers to a new world of fruit breeding, very seldom ventured by breeders.


Biotechnology: Prospects and Applications

Biotechnology: Prospects and Applications

Author: R.K. Salar

Publisher: Springer Science & Business Media

Published: 2014-02-06

Total Pages: 313

ISBN-13: 8132216830

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Biotechnology: Prospects and Applications covers the review of recent developments in biotechnology and international authorship presents global issues that help in our understanding of the role of biotechnology in solving important scientific and societal problems for the benefit of mankind and environment. A balanced coverage of basic molecular biology and practical applications, relevant examples, colored illustrations, and contemporary applications of biotechnology provide students and researchers with the tools and basic knowledge of biotechnology. In our effort to introduce students and researchers to cutting edge techniques and applications of biotechnology, we dedicated specific chapters to such emerging areas of biotechnology as Emerging Dynamics of Brassinosteroids Research, Third generation green energy, Bioremediation, Metal Organic Frameworks: New smart materials for biological application, Bioherbicides, Biosensors, Fetal Mesenchymal Stem Cells and Animal forensics. Biotechnology: Prospects and Applications will be highly useful for students, teachers and researchers in all disciplines of life sciences, agricultural sciences, medicine, and biotechnology in universities, research stations and biotechnology companies. The book features broader aspects of the role of biotechnology in human endeavor. It also presents an overview of prospects and applications while emphasizing modern, cutting-edge, and emerging areas of biotechnology. Further, it provides the readers with a comprehensive knowledge of topics in food and agricultural biotechnology, microbial biotechnology, environmental biotechnology and animal biotechnology. The chapters have been written with special reference to the latest developments in above broader areas of biotechnology that impact the biotechnology industry. A list of references at the end of each chapter is provided for the readers to learn more about a particular topic. Typically, these references include basic research, research papers, review articles and articles from the popular literature.


The Coconut Palm (Cocos nucifera L.) - Research and Development Perspectives

The Coconut Palm (Cocos nucifera L.) - Research and Development Perspectives

Author: V. Krishnakumar

Publisher: Springer

Published: 2019-02-15

Total Pages: 850

ISBN-13: 9811327548

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Since the publication of "The coconut palm - A monograph" in 1960, considerable information has been accrued on the crop through work at research institutes, international organisations and development agencies. Although coconut cultivation is spread over 93 countries, providing employment and creating livelihood opportunities to 64 million families around the globe, smallholder coconut farmers are now facing numerous challenges. The wide gap between the potential and actual yield is a major concern, and as such it is necessary to disseminate knowledge in order to implement research findings. Coconut research in India, one of the leading coconut producing countries, is celebrating its centenary, making this an opportune time to review the research and development advances and the relevant technologies. This detailed, comprehensive book covers all aspects of coconut, from the origins to cultivation, breeding, physiology and value addition, as well as subjects of topical interest like nutrition and health, biotechnology, and climate change and carbon sequestration. Written by leading experts in the fields it emphasises that the livelihood of the small coconut landholders is the ultimate aim of scientists and developmental agencies, and outlines various important strategies to make coconut farming more remunerative globally. It discusses work in all the major coconut growing countries and outlines suggestions for international cooperation. Research work on the crop is comparatively difficult because of its perennial nature, longevity, height, long juvenile phase, large sized nuts, cross pollination and seed propagation. As these special features necessitate greater investment of resources, time and land, it is all the more imperative that research is not duplicated and the information and experience becoming available around the world is shared so that it can be fully utilised. In this context periodic publications, compiling all the available information on coconut assume greater significance. This book is therefore of great value to researchers, students, extension workers, developmental agencies and progressive farmers.


Turmeric (Curcuma longa L.) and Ginger (Zingiber officinale Rosc.) - World's Invaluable Medicinal Spices

Turmeric (Curcuma longa L.) and Ginger (Zingiber officinale Rosc.) - World's Invaluable Medicinal Spices

Author: Kodoth Prabhakaran Nair

Publisher: Springer Nature

Published: 2019-10-25

Total Pages: 577

ISBN-13: 3030291898

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This book discusses the various aspects, from production to marketing of turmeric and ginger, the world’s two most important and invaluable medicinal spice crops. The book begins with their origin and history, global spread, and goes on to describe the botany, production agronomy, fertilizer practices, pest management, post-harvest technology, pharmacology and nutraceutical uses. The book presents the economy, import-export and world markets involved with reference to turmeric and ginger. It would be a benchmark and an important reference source for scientists, students, both undergraduate and post graduate, studying agriculture and food sciences and policy makers. It would be of great interest to professionals and industry involved in spice trade.


Microbiological Advancements for Higher Altitude Agro-Ecosystems & Sustainability

Microbiological Advancements for Higher Altitude Agro-Ecosystems & Sustainability

Author: Reeta Goel

Publisher: Springer Nature

Published: 2020-03-13

Total Pages: 493

ISBN-13: 9811519021

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The book explores the challenges and opportunities associated with high-altitude agro-ecosystems and the factors that influence them. It discusses the various indigenous agricultural practices and approaches, as well as the microbiology of mountain & hill agro-ecosystems, providing a comprehensive overview of the various factors that control the microbiome at high altitudes. The contributions examine microbiological advances, such as use of “omics” technologies for hill agriculture and environmental sustainability, and explore the use of nanotechnology for agricultural and environmental sustainability at higher altitudes. The book also describes various aspects of low-temperature microbiology in the context of high-altitude farming and environmental sustainability.