Viral Genes and Plant Pathogenesis

Viral Genes and Plant Pathogenesis

Author: Thomas P. Pirone

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 229

ISBN-13: 1461234247

DOWNLOAD EBOOK

The chapters in this book represent detailed versions of papers presented at the Symposium on Viral Genes and Plant Pathogenesis held at Lexington, Kentucky on October 16 and 17, 1989. In selecting topics and authors, we attempted to have represented a spectrum of systems which are at the forefront of research on plant virus genes and gene products, particularly as they relate to plant disease. The book also contains pertinent discussion of the papers presented at the symposium, as well as summaries, observations and projections of future research directions prepared by the session chairmen. We wish to express our appreciation to Dr. D. L. Davis, of the RI. Reynolds Tobacco Company for suggesting the organization of the Symposium and the publication of the proceedings, and to the R 1. Reynolds Tobacco Company for the financial support which made the symposium possible. We also wish to thank those of our colleagues in the Department of Plant Pathology, University of Kentucky, who very ably and in many ways contributed to the organization and conduct of the conference. Thomas P. Pirone lohnG. Shaw v Contents Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xi INDUCTION OF HOST GENES BY THE HYPERSENSITIVE RESPONSE OF TOBACCO TO VIRUS INFECTION J. F. Bol, C. M. A. van Rossum, Bl. C. Cornelissen and H. J. M. Linthorst . . . . . . . . . . . . . . . . . l COAT PROTEIN MEDIATED RESISTANCE IN TRANSGENIC PLANTS Roger N. Beachy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 STRUCTURE AND FUNCTIONS OF TOBACCO MOSAIC VIRUS RNA Yoshimi Okada, Tetsuo Meshi, and Yuichiro Watanabe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .


Virus-Induced Gene Silencing

Virus-Induced Gene Silencing

Author: Annette Becker

Publisher: Humana Press

Published: 2013-02-06

Total Pages: 0

ISBN-13: 9781627032773

DOWNLOAD EBOOK

Plants are amazing organisms to study, some are important sources for pharmaceuticals, and others can help to elucidate molecular mechanisms required for a plant’s development and its interactions with the biotic or abiotic environment. Functional genomics is vastly lagging behind the speed of genome sequencing as high-throughput gene function assays are difficult to design, specifically for non-model plants. Bioinformatics tools are useful for gene identification and annotation but are of limited value for predictions concerning gene functions as gene functions are uncovered best by experimental approaches. Virus-Induced-Gene-Silencing (VIGS) is an easy to use, fast, and reliable method to achieve down regulation of target gene expression. Virus-Induced Gene Silencing: Methods and Protocols provides detailed protocols for VIGS experiments in several plant species including model and non-model plants. Also included in this book are recently developed protocols for VIGS-derived microRNA production in the plant or protein over expression, as well as chapters devoted to summarizing the molecular mechanisms of VIGS action and the vector systems developed so far. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Virus-Induced Gene Silencing: Methods and Protocols serves as a valuable resource for researchers from diverse fields of plant biology interested in experimental approaches to analyzing gene functions.


Molecular Biology of Plant Nuclear Genes

Molecular Biology of Plant Nuclear Genes

Author: Indra Vasil

Publisher: Elsevier

Published: 2012-12-02

Total Pages: 521

ISBN-13: 0323147321

DOWNLOAD EBOOK

Cell Culture and Somatic Cell Genetics of Plants, Volume 6: Molecular Biology of Plant Nuclear Genes focuses on the spectacular and rapid advances in the molecular biology and genetics of plants. This book consists of 19 chapters. Chapters 1 to 5 describe the most commonly used approaches for the genetic transformation of plants. The light-inducible and tissue-organ-specific genes are discussed in Chapters 6 to 11. In Chapters 12 to 14, the genes regulating phytohormone synthesis, heat shock proteins, and nodulation in legume roots are treated, while in Chapters 15 to 16, the relationship between chromatin structure and gene expression and molecular biology of plant RNA viruses are analyzed. The development of transgenic plants resistant to viruses, insects, and herbicides is dealt with in the last three chapters. This volume is suitable for plant molecular biologist, genetic engineers, and researchers concerned with plant cell and tissue culture.


100 Years of Virology

100 Years of Virology

Author: Charles H. Calisher

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 222

ISBN-13: 3709164257

DOWNLOAD EBOOK

One hundred years ago, when Martinus W. Beijerinck in Delft and Friedrich Loeffler on Riems Island discovered a new class of infectious agents in plants and animals, a new discipline was born. This book, a compilation of papers written by well-recognized scientists, gives an impression of the early days, the pioneer period and the current state of virology. Recent developments and future perspectives of this discipline are sketched against a historic background. With contributions by A. Alcami, D. Baulcombe, F. Brown, L. W. Enquist, H. Feldmann, A. Garcia-Sastre, D. Griffiths, M. C. Horzinek, A. van Kammen, H.-D. Klenk, F. A. Murphy, T. Muster, R. O'Neill, P. Palese, C. Patience, R. Rott, H.- P. Schmiedebach, S. Schneider-Schaulies, G. L. Smith, J. A. Symons, Y. Takeuchi, V. ter Meulen, P. J. W. Venables, V. E. Volchkov, V. A. Volchkova, R. A. Weiss, W. Wittmann, H. Zheng.


Structure and Function of Plant Genomes

Structure and Function of Plant Genomes

Author: Orio Ciferri

Publisher: Springer

Published: 1983-10

Total Pages: 522

ISBN-13:

DOWNLOAD EBOOK

This volume contains the presentations of the principal speakers at the NATO Advanced Study Institute held at Porto Portese, Italy,23 August - 2 September, 1982. This meeting was the third in a series devoted to the molecular biology of plants. The initial meeting was held in Strasbourg, France in 1976 (J. Weil and L. Bogorad, organizers), and the second in Edinburgh, Scotland in 1979 (C. Leaver, organizer). As in these previous meetings, we have attempted to cover the major topics of plant molecular biology so as to promote the integration of information emerging at an accelerating rate from the various sub-disciplines of the field. In addition, we have introduced several topics, unique to higher plants, that have not yet been approached with the tools of molec ular biology, but that should present new and important aspects of plants amenable to study in terms of DNA -+ RNA -+ Protein. This meeting also served to inaugerate the new International Society for Plant Molecular Biology. The need for this society is, like the NATO meetings themselves, an indication of the growth, vitalitv and momentum of this field of research.


Plant DNA Infectious Agents

Plant DNA Infectious Agents

Author: Thomas Hohn

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 355

ISBN-13: 3709169771

DOWNLOAD EBOOK

There has been recent rapid progress in the transformation of plants with foreign DNA, making use either of the natural routes of genetic invasion that viruses and bacteria have developed, or of chemical, mechanical and electrical tricks to make plant protoplast membranes permeable to nucleic acids. Genes integrated into plant virus genomes can be carried systemi cally from the initial site of infection into the rest of the plant. Genes placed between the borders of Agrobacterium tumefaciens T-DNA can be transferred into single cells or plant tissue, which then divides to produce wound calli, or as in the case of an Agrobacterium rhizogenes infection, grow out into new roots. Calli and roots can be grown into whole plants. If virus genomes are placed between the T-DNA borders, a very effective infectious route, termed "agroinfection", is established. Once inside a pro toplast, DNA finds its way into the nucleus where it can finally integrate into the resident chromosome and be expressed. Whether it can also find its way into chloroplasts is not yet clear, but at least translation products can be targeted into this organelle. Regeneration of whole organisms from single cells is a special feature of plants and offers a unique tool to study genes in a multicellular organism. In addition, as in animal cells, transcription and translation of trans forming genes can be studied in plant cells during "transient expression".


Stress-Induced Gene Expression in Plants

Stress-Induced Gene Expression in Plants

Author: Amarjit Basra

Publisher: CRC Press

Published: 1994-09-21

Total Pages: 306

ISBN-13: 9783718654666

DOWNLOAD EBOOK

Researchers from North America and Western Europe discuss the state of the art research on gene expression in plants as affected by various stresses such as water deficit, seed dessication, anoxia, salinity, temperature extremes, heavy metals, air pollutants, and infection by pathogens. They also look at the possibilities of exploiting genes that regulate ozone resistance and the ingenious molecular strategies that have been developed by plants for dealing with pathogen attack. Annotation copyright by Book News, Inc., Portland, OR


Virus-Induced Gene Silencing

Virus-Induced Gene Silencing

Author: Annette Becker

Publisher: Humana Press

Published: 2013-02-06

Total Pages: 221

ISBN-13: 9781627032797

DOWNLOAD EBOOK

Plants are amazing organisms to study, some are important sources for pharmaceuticals, and others can help to elucidate molecular mechanisms required for a plant’s development and its interactions with the biotic or abiotic environment. Functional genomics is vastly lagging behind the speed of genome sequencing as high-throughput gene function assays are difficult to design, specifically for non-model plants. Bioinformatics tools are useful for gene identification and annotation but are of limited value for predictions concerning gene functions as gene functions are uncovered best by experimental approaches. Virus-Induced-Gene-Silencing (VIGS) is an easy to use, fast, and reliable method to achieve down regulation of target gene expression. Virus-Induced Gene Silencing: Methods and Protocols provides detailed protocols for VIGS experiments in several plant species including model and non-model plants. Also included in this book are recently developed protocols for VIGS-derived microRNA production in the plant or protein over expression, as well as chapters devoted to summarizing the molecular mechanisms of VIGS action and the vector systems developed so far. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Virus-Induced Gene Silencing: Methods and Protocols serves as a valuable resource for researchers from diverse fields of plant biology interested in experimental approaches to analyzing gene functions.