Stable Isotope Techniques in the Study of Biological Processes and Functioning of Ecosystems

Stable Isotope Techniques in the Study of Biological Processes and Functioning of Ecosystems

Author: M.J. Unkovich

Publisher: Springer Science & Business Media

Published: 2013-03-14

Total Pages: 312

ISBN-13: 940159841X

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In the last two decades technological advances in isotope ratio mass spectrometry have been very rapid, opening up new possibilities for analysis of biological and environmental materials. The new instrumentation has facilitated faster analysis of samples via automated sample preparation and multi-isotope analysis of single samples, resulting in considerable cost savings, and enabling access to isotope analysis for many more researchers. These changes are reflected in the rapidly growing international literature on stable isotopes. While there have been some excellent books and review papers aimed at interpreting isotope signals in biology and environmental science, there have been fewer attempts to provide practical tools for researchers making forays into this exciting new arena. This book aims to address this inadequacy by providing a set of practical guidelines for the application of a range of novel and well proven stable isotope techniques to the fields of plant physiological ecology, agriculture, marine ecology and palaeoecology. The book is the outcome of a weeklong workshop held under the auspices of the Cooperative Research Centre for Legumes in Mediterranean Agriculture (CLIMA 1992 - 2000) at The University of Western Australia and the CSIRO Floreat Laboratories, Perth, Western Australia, in February 1999. The workshop was designed to provide practical tools and experiences for researchers and students concerned with how one goes about using stable isotopes in field investigations.


Stable Isotopes and Biosphere - Atmosphere Interactions

Stable Isotopes and Biosphere - Atmosphere Interactions

Author: Lawrence B Flanagan

Publisher: Elsevier

Published: 2004-12-15

Total Pages: 334

ISBN-13: 0080525288

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The emerging multidisciplinary field of earth system science sets out to improve our understanding functioning ecosystems, at a global level across the entire planet. Stable Isotopes and Biosphere - Atmosphere Interactions looks to one of its most powerful tools — the application of stable isotope analyses — to understanding biosphere-atmosphere exchange of the greenhouse gases, and synthesizes much of the recent progress in this work. Stable Isotopes and Biosphere - Atmosphere Interactions describes recent progress in understanding the mechanisms, processes and applications of new techniques. It makes a significant contribution to the emerging, multidisciplinary study of the Earth as an interacting system. This book will be an important reference for students and researchers in biology, ecology, biogeochemistry, meteorology, and atmospheric science and will be invaluable for anyone with any interest in the future of the planet. - Describes applications of new stable isotope techniques to the emerging fields of earth system science and global change - Illustrates advances in scaling of physiological processes from leaf/soil to the global scale - Contains state-of-the-art, critical reviews written by international researchers and experts


Compound-specific Stable Isotope Analysis

Compound-specific Stable Isotope Analysis

Author: Maik A Jochmann

Publisher: Royal Society of Chemistry

Published: 2015-11-09

Total Pages: 306

ISBN-13: 1782626107

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The use of Compound-specific Stable Isotope Analysis (CSIA) is increasing in many areas of science and technology for source allocation, authentication, and characterization of transformation reactions. Until now, there have been no textbooks available for students with an analytical chemical background or basic introductory books emphasising the instrumentation and theory. This book is the first to focus solely on stable isotope analysis of individual compounds in sometimes complex mixtures. It acts as both a lecture companion for students and a consultant for advanced scientists in fields including forensic and environmental science. The book starts with a brief history of the field before going on to explain stable isotopes from scratch. The different ways to express isotope abundances are introduced together with isotope effects and isotopic fractionation. A detailed account of the required technical equipment and general procedures for CSIA is provided. This includes sections on derivatization and the use of microextraction techniques in GC-IRMS. The very important topic of referencing and calibration in CSIA is clearly described. This differs from approaches used in quantitative analysis and is often difficult for the newcomer to comprehend. Examples of successful applications of CSIA in food authenticity, forensics, archaeology, doping control, environmental science, and extraterrestrial materials are included. Applications in isotope data treatment and presentation are also discussed and emphasis is placed on the general conclusions that can be drawn from the uses of CSIA. Further instrumental developments in the field are highlighted and selected experiments are introduced that may act as a basis for a short practical course at graduate level.


Stable Isotope Ecology

Stable Isotope Ecology

Author: Brian Fry

Publisher: Springer Science & Business Media

Published: 2007-01-15

Total Pages: 318

ISBN-13: 0387337458

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A solid introduction to stable isotopes that can also be used as an instructive review for more experienced researchers and professionals. The book approaches the use of isotopes from the perspective of ecological and biological research, but its concepts can be applied within other disciplines. A novel, step-by-step spreadsheet modeling approach is also presented for circulating tracers in any ecological system, including any favorite system an ecologist might dream up while sitting at a computer. The author’s humorous and lighthearted style painlessly imparts the principles of isotope ecology. The online material contains color illustrations, spreadsheet models, technical appendices, and problems and answers.


Isoscapes

Isoscapes

Author: Jason B. West

Publisher: Springer Science & Business Media

Published: 2009-11-25

Total Pages: 495

ISBN-13: 9048133548

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Stable isotope ratio variation in natural systems reflects the dynamics of Earth systems processes and imparts isotope labels to Earth materials. Carbon isotope ratios of atmospheric CO2 record exchange of carbon between the biosphere and the atmosphere; the incredible journeys of migrating monarchs is documented by hydrogen isotopes in their wings; and water carries an isotopic record of its source and history as it traverses the atmosphere and land surface. Through these and many other examples, improved understanding of spatio-temporal isotopic variation in Earth systems is leading to innovative new approaches to scientific problem-solving. This volume provides a comprehensive overview of the theory, methods, and applications that are enabling new disciplinary and cross-disciplinary advances through the study of "isoscapes": isotopic landscapes. "This impressive new volume shows scientists deciphering and using the natural isotope landscapes that subtly adorn our spaceship Earth.", Brian Fry, Coastal Ecology Institute, Louisiana State University, USA "An excellent timely must read and must-have reference book for anybody interested or engaged in applying stable isotope signatures to questions in e.g. Anthropology, Biogeochemistry, Ecology, or Forensic Science regarding chronological and spatial movement, changes, or distribution relating to animals, humans, plants, or water.", Wolfram Meier-Augenstein, Centre for Anatomy & Human Identification, University of Dundee, UK "Natural resources are being affected by global change, but exactly where, how, and at what pace? Isoscapes provide new and remarkably precise answers.", John Hayes, Woods Hole Oceanographic Institution, USA "This exciting volume is shaping a new landscape in environmental sciences that is utilizing the remarkable advances in isotope research to enhance and extend the capabilities of the field.", Dan Yakir, Weizmann Institute of Science, Israel


Stable Isotope Probing

Stable Isotope Probing

Author: Marc G. Dumont

Publisher: Humana

Published: 2020-08-13

Total Pages: 247

ISBN-13: 9781493997237

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This book provides definitive methods to perform stable isotope probing (SIP) experiments, covering a wide spectrum of stable isotope techniques used in microbial ecology, such as methods to target and analyze labeled DNA, rRNA, mRNA, protein, and PLFA. Protocols to study stable isotope fractionation by microbial pathways, the analysis of labeled communities with Raman microscopy, Chip-SIM, as well as quantitative SIP (qSIP) and high-resolution SIP (HR-SIP) are also featured. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Stable Isotope Probing: Methods and Protocols provides readers with up-to-date protocols ranging from basic to the most sophisticated applications of SIP and will benefit anyone pursuing this exciting area of study.


Stable Isotopes in Ecological Research

Stable Isotopes in Ecological Research

Author: P.W. Rundel

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 536

ISBN-13: 1461234980

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The analysis of stable isotope ratios represents one of the most exciting new technical advances in environmental sciences. In this book, leading experts offer the first survey of applications of stable isotope analysis to ecological research. Central topics are - plant physiology studies - food webs and animal metabolism - biogeochemical fluxes. Extensive coverage is given to natural isotopes of carbon, hydrogen, oxygen, nitrogen, sulfur, and strontium in both terrestrial and marine ecosystems. Ecologists of diverse research interests, as well as agronomists, anthropologists, and geochemists will value this overview for its wealth of information on theoretical background, experimental approaches, and technical design of studies utilizing stable isotope ratios.


Stable Isotopes in Ecology and Environmental Science

Stable Isotopes in Ecology and Environmental Science

Author: Robert Michener

Publisher: John Wiley & Sons

Published: 2008-04-15

Total Pages: 592

ISBN-13: 0470691174

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This book highlights new and emerging uses of stable isotope analysis in a variety of ecological disciplines. While the use of natural abundance isotopes in ecological research is now relatively standard, new techniques and ways of interpreting patterns are developing rapidly. The second edition of this book provides a thorough, up-to-date examination of these methods of research. As part of the Ecological Methods and Concepts series which provides the latest information on experimental techniques in ecology, this book looks at a wide range of techniques that use natural abundance isotopes to: follow whole ecosystem element cycling understand processes of soil organic matter formation follow the movement of water in whole watersheds understand the effects of pollution in both terrestrial and aquatic environments study extreme systems such as hydrothermal vents follow migrating organisms In each case, the book explains the background to the methodology, looks at the underlying principles and assumptions, and outlines the potential limitations and pitfalls. Stable Isotopes in Ecology and Environmental Science is an ideal resource for both ecologists who are new to isotopic analysis, and more experienced isotope ecologists interested in innovative techniques and pioneering new uses.


Handbook of Stable Isotope Analytical Techniques

Handbook of Stable Isotope Analytical Techniques

Author: Pier A. de Groot

Publisher: Elsevier

Published: 2004-10-27

Total Pages: 1265

ISBN-13: 0080533272

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(Parent with price) Volume I contains subjective reviews, specialized and novel technique descriptions by guest authors. Part 1 includes contributions on purely analytical techniques and Part 2 includes matters such as development of mass spectrometers, stability of ion sources, standards and calibration, correction procedures and experimental methods to obtain isotopic fractionation factors.Volume II will be available in 2005.


Principles of Stable Isotope Distribution

Principles of Stable Isotope Distribution

Author: Robert E. Criss

Publisher: Oxford University Press

Published: 1999-06-03

Total Pages: 264

ISBN-13: 0190283580

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This book presents a quantitative treatment of the theory and natural variations of light stable isotopes. It discusses isotope distribution in the context of fractionation processes, thermodynamics, mass conservation, exchange kinetics, and diffusion theory, and includes more than 100 original equations. The theoretical principles are illustrated with natural examples that emphasize oxygen and hydrogen isotope variations in natural waters, terrestrial and extraterrestrial rocks, and hydrothermal systems. New data on meteoric precipitation, rivers, springs, formation fluids, and hydrothermal systems are included in relation to various natural phenomena. Essentially, this book seeks to reconnect the diverse phenomenological observations of isotope distribution to the quantitative theories of physical chemistry and the language of differential equations. It may serve as a textbook for advanced students, as a research reference, or as a quick source of information. The book is organized into five chapters, each followed by suggested quantitative problems and a short reference list. The three theoretical chapters progress from an elementary review of the physical chemistry of stable isotopes, to the thermodynamics of isotopic compounds, and finally to the calculation of isotope distribution in dynamic systems. The third and fifth chapters emphasize oxygen and hydrogen isotope variations in Earth's hydrosphere and lithosphere, constituting the most important examples of the theoretical principles. Appendices provide data on atomic weights of light elements, physical constants, mathematical relationships, and isotopic fractionation factors.