Spacelab J Air Filter Debris Analysis

Spacelab J Air Filter Debris Analysis

Author: National Aeronautics and Space Adm Nasa

Publisher: Independently Published

Published: 2019-01-04

Total Pages: 30

ISBN-13: 9781792838880

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Filter debris from the Spacelab module SLJ of STS-49 was analyzed for microbial contamination. Debris for cabin and avionics filters was collected by Kennedy Space Center personnel on 1 Oct. 1992, approximately 5 days postflight. The concentration of microorganisms found was similar to previous Spacelab missions averaging 7.4E+4 CFU/mL for avionics filter debris and 4.5E+6 CFU/mL for the cabin filter debris. A similar diversity of bacterial types was found in the two filters. Of the 13 different bacterial types identified from the cabin and avionics samples, 6 were common to both filters. The overall analysis of these samples as compared to those of previous missions shows no significant differences. Obenhuber, Donald C. NASA-CR-192569, NAS 1.26:192569, STS-49-SLJ NAS8-37814...


Analysis of Debris from Spacelab Space Life Sciences-1

Analysis of Debris from Spacelab Space Life Sciences-1

Author: National Aeronautics and Space Administration (NASA)

Publisher: Createspace Independent Publishing Platform

Published: 2018-07-06

Total Pages: 92

ISBN-13: 9781722232955

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Airborne microbiological and particulate contamination generated aboard Spacelab modules is a potential safety hazard. In order to shed light on the characteristics of these contaminants, microbial and chemical/particulate analyses were performed on debris vacuumed from cabin and avionics air filters in the Space Life Sciences-1 (SLS-1) module of the Space Transportation System 40 (STS-40) mission 1 month after landing. The debris was sorted into categories (e.g., metal, nonmetal, hair/fur, synthetic fibers, food particles, insect fragments, etc.). Elemental analysis of particles was done by energy dispersive analysis of x rays (metals) and Fourier transform infrared spectroscopy (nonmetals). Scanning electron micrographs were done of most particles. Microbiological samples were grown on R2A culture medium and identified. Clothing fibers dominated the debris by volume. Other particles, all attributed to the crew, resulted from abrasions and impacts during missions operations (e.g., paint chips, plastic, electronic scraps and clothing fibers). All bacterial species identified are commonly found in the atmosphere or on the human body. Bacillus sp. was the most frequently seen bacterium. One of the bacterial species, Enterobacter agglomerans, could cause illness in crew members with depressed immune systems. Caruso, S. V. and Rodgers, E. B. and Huff, T. L. Marshall Space Flight Center...


Microbiological Analysis of Debris from Sts-42 IML-1 by Direct Plating of Rinse Waters

Microbiological Analysis of Debris from Sts-42 IML-1 by Direct Plating of Rinse Waters

Author: National Aeronautics and Space Adm Nasa

Publisher: Independently Published

Published: 2018-12-31

Total Pages: 38

ISBN-13: 9781792856709

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Microbial analysis of air filter debris from the Spacelab International Microgravity Laboratory-1 (IML-1) mission was performed via direct plating of rinse waters on a battery of selective and nonselective nutrient agars. Microbial isolates were identified using Minitek and Biolog technologies. Twenty-four types of bacteria were recovered and classified; a similar number of fungal types was observed, but these were not identified. This procedure can provide information about the proportions of organism types present at the time of debris collection. Smithers, G. A. Marshall Space Flight Center NASA-TM-108375, NAS 1.15:108375 ...