Scanning Electron Microscopy of Vascular Casts: Methods and Applications
Scanning Electron Microscopy of Vascular Casts: Methods and Applications

Author: P. Motta

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 385

ISBN-13: 1461534887

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Recently, attention has been called to the role that microvascular organization plays in the functional morphology of all organs and tissues, both in normal and pathological conditions. Since its development by Murakami, the corrosion cast method for scanning electron microscopy has come to be considered one of the most efficient means in clarifying the three-dimensional features of the microcirculation of organs and tissues. Scanning Electron Microscopy of Vascular Casts: Methods and Applications was planned to supply fundamental and new information regarding microcirculation studies to general biologists, anatomists, pathologists and clinicians. The contributions to this volume, contain original findings and excellent electron micrographs obtained by using recently improved corrosion cast methods. The rich variety of papers in this book will be useful to many, and will provide both the basic and clinically oriented readers with good ideas, suggestions, and original and worthwhile information.

Scanning Electron Microscopy of Vascular Casts: Methods and Applications
Scanning Electron Microscopy of Vascular Casts: Methods and Applications

Author: P. Motta

Publisher: Springer

Published: 2012-10-30

Total Pages: 390

ISBN-13: 9781461365464

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Recently, attention has been called to the role that microvascular organization plays in the functional morphology of all organs and tissues, both in normal and pathological conditions. Since its development by Murakami, the corrosion cast method for scanning electron microscopy has come to be considered one of the most efficient means in clarifying the three-dimensional features of the microcirculation of organs and tissues. Scanning Electron Microscopy of Vascular Casts: Methods and Applications was planned to supply fundamental and new information regarding microcirculation studies to general biologists, anatomists, pathologists and clinicians. The contributions to this volume, contain original findings and excellent electron micrographs obtained by using recently improved corrosion cast methods. The rich variety of papers in this book will be useful to many, and will provide both the basic and clinically oriented readers with good ideas, suggestions, and original and worthwhile information.

Scanning Electron Microscopy for the Life Sciences
Scanning Electron Microscopy for the Life Sciences

Author: Heide Schatten

Publisher: Cambridge University Press

Published: 2013

Total Pages: 275

ISBN-13: 0521195993

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A guide to modern scanning electron microscopy instrumentation, methodology and techniques, highlighting novel applications to cell and molecular biology.

Handbook of Vascular Biology Techniques
Handbook of Vascular Biology Techniques

Author: Mark Slevin

Publisher: Springer

Published: 2015-03-27

Total Pages: 466

ISBN-13: 9401797161

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A wide range of research methods for the study of vascular development, from basic laboratory protocols to advanced technologies used in clinical practice, are covered in this work. A range of methodologies such as molecular imaging platforms and signalling analysis, along with tumour models are collated here. Four sections explore in vitro techniques, in vivo and ex vivo manipulations, imaging and histological analysis and other novel techniques in vascular biology. Readers will discover basic methodologies used for analysis of endothelial cell growth in vitro, including co-culture models of vessel formation. Authors also explore isolation and purification of cells and methods for analysis of data and visualization of localized vasculature with modern imaging platforms. Both animal models and human disease are covered in this work. Each chapter contains helpful sections on trouble shooting, additional notes and links, supporting the reader to carry out protocols. This book will appeal to students, researchers and medical professionals working in all vascular-linked fields such as cardio- and cerebrovascular, cancer and dementia.

Color Atlas of Adult Xenopus laevis
Color Atlas of Adult Xenopus laevis

Author: Alois Lametschwandtner

Publisher: Springer Nature

Published: 2022-11-18

Total Pages: 293

ISBN-13: 3031051106

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This atlas offers stunning color electron scanning micrographs and exceptional light microscopy pictures of capillaries, vessels and diverse histomorphological tissues and organs of Xenopus laevis. The model organisms Xenopus laevis serves to study basic biological questions related to growth, differentiation, maturation, and regression of cells, tissues and organs. Xenopus and human genomes have long stretches of gene collinearity, and 79% of identified human disease genes have a verified ortholog in Xenopus. Thus, this atlas will be a powerful tool for anatomists, morphologists, histologists and physiologists interested in normal and pathologically altered organs and tissue; and to all researchers, who wish to learn more about the microvascular anatomy of this vertebrate model organism.

The Textbook of Angiogenesis and Lymphangiogenesis: Methods and Applications
The Textbook of Angiogenesis and Lymphangiogenesis: Methods and Applications

Author: Enrique Zudaire

Publisher: Springer Science & Business Media

Published: 2012-11-29

Total Pages: 436

ISBN-13: 9400745818

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Angiogenesis and lymphangiogenesis have become attractive targets for drug therapy because of their key roles in a broad spectrum of pathological disease states ranging from macular degeneration to tumor growth and metastasis. A substantial increase in the research effort over the past decade has deepened our understanding of the basic mechanisms underlying angiogenesis and lymphangiogenesis, promoting the development of promising therapeutics for the clinical management of vascular-related diseases. These extraordinary advancements have been built upon a vast array of diverse analytical techniques developed globally throughout the field. Over the years, these methods have evolved to suit the specific needs of different researchers and experimental scenarios, resulting in a myriad of technical variants of basic assay approaches. "The Textbook of Angiogenesis and Lymphangiogenesis: Methods and Applications" is an up-to-date comprehensive textbook on angiogenesis and lymphangiogenesis techniques and applications. This volume is designed to embody the collective works of experts in the clinical as well as the basic research arenas who have significantly contributed to the development and application of techniques in all areas of angiogenesis and lymphangiogenesis. Each chapter introduces and discusses one or a group of closely related techniques and convey step-by-step protocol information and detailed technical guidance to the reader. Emphasis has been placed on explanatory illustrations, critical technical steps as well as divulging information on the benefits and caveats of specific practices related to the methods discussed. This manual is intended to serve as a written guide for both newcomers and established professionals in the field.

Microvascular Corrosion Casting in Scanning Electron Microscopy
Microvascular Corrosion Casting in Scanning Electron Microscopy

Author: S.H. Aharinejad

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 518

ISBN-13: 3709192307

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Several methods have been used to demonstrate the vasculature of different organs in man and other species. Many attempts to evaluate the precise microangioarchitecture of organ systems remained unproductive, others were controversial. The development of electron microscope in thirties opend new perspectives in researching microvascular systems. Transmission electron microscopy provided a two-dimensional view on microcirculatory system at higher magnifications, however, its standardization was delayed unnecessarily. The use of methyl methacrylate and related compounds for obtaining replicas of vascular beds, and their study in scanning electron microscope opened a new window in micromorphological research. For the first time, a three-dimensional image analysis of the vascular system was possible. The microvascular corrosion casting method has meanwhile attracted the interest of many contemporary scientists. Its application to medical and biological problems justify it to be used as a routine method for microvascular investigations. The first investigators who used this method, focused either on methodological details or they dealt with the normal microanatomy of organs. The advantages of this method in demonstrating pathological microvascular patterns are also evident.

Methods in Endothelial Cell Biology
Methods in Endothelial Cell Biology

Author: Hellmut G. Augustin

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 381

ISBN-13: 3642187250

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Endothelial cell biology has developed into a vibrant discipline and has become a critical instrument to study several disease processes on the cellular and molecular level. It is now widely recognized that dysfunctions of normal endothelial cell homeostasis are involved in some of the most important human diseases, including ischemic heart diseases, hypertension, atherosclerosis, tumors, diabetes, arthritis, and inflammation. Further, the increasing importance and recognition of the field of vascular biology in general requires in vitro and in vivo techniques in order to address the complex questions. Methods in Endothelial Cell Biology is a comprehensive practical "how-to"-guide summarizing the most relevant established techniques as well as a number of new emerging techniques. Easy-to-follow reliable protocols provide a useful lab bench resource for the experienced researcher and newcomer to the field.

Methods of Preparation for Electron Microscopy
Methods of Preparation for Electron Microscopy

Author: David G. Robinson

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 204

ISBN-13: 364248848X

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In 1939, when the electron optics laboratory of Siemens & Halske Inc. began to manufacture the first electron microscopes, the biological and medical profes sions had an unexpected instrument at their disposal which exceeded the reso lution of the light microscope by more than a hundredfold. The immediate and broad application of this new tool was complicated by the overwhelming prob lems inherent in specimen preparation for the investigation of cellular struc tures. The microtechniques applied in light microscopy were no longer appli cable, since even the thinnest paraffin layers could not be penetrated by electrons. Many competent biological and medical research workers expressed their anxiety that objects in high vacuum would be modified due to complete dehydration and the absorbed electron energy would eventually cause degrada tion to rudimentary carbon backbones. It also seemed questionable as to whether it would be possible to prepare thin sections of approximately 0. 5 11m from heterogeneous biological specimens. Thus one was suddenly in posses sion of a completely unique instrument which, when compared with the light microscope, allowed a 10-100-fold higher resolution, yet a suitable preparation methodology was lacking. This sceptical attitude towards the application of electron microscopy in bi ology and medicine was supported simultaneously by the general opinion of colloid chemists, who postulated that in the submicroscopic region of living structures no stable building blocks existed which could be revealed with this apparatus.

Decellularization Methods of Tissue and Whole Organ in Tissue Engineering
Decellularization Methods of Tissue and Whole Organ in Tissue Engineering

Author: Abdol-Mohammad Kajbafzadeh

Publisher: Springer Nature

Published: 2021-09-28

Total Pages: 266

ISBN-13: 3030827356

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This contributed volume is the first of a series that introduces safe, feasible, and practical decellularization and recellularization techniques for tissue and organ reconstruction. We have put special emphasis on the research areas most likely to develop well-engineered scaffolds for tissue and organ engineering, while presenting easily applicable bench-to-bedside approaches highlighting the latest technical innovations in the field. This book includes both a fundamental discussion for a broad understanding of the basis of tissue repair and substitution, as well as chapters written by world renowned specialists from 20 countries providing deeper discussions and analysis of related sub disciplines. Within these pages, the reader will find state-of-the-art protocols and current clinical challenges in cell and tissue biology, including accurate and comprehensive information on extracellular matrices, natural biomaterials, tissue dynamics, morphogenesis, stem cells, cellular fate progressions, cell and tissue properties for in-vitro and in-vivo applications. This comprehensive and carefully organized treatise provides a clear framework for graduate students and postdoctoral researchers new to the field, but also for researchers and practitioners looking to expand their knowledge on tissue and organ reconstruction.