Plant Gene Silencing
Plant Gene Silencing

Author: M.A. Matzke

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 297

ISBN-13: 9401141835

DOWNLOAD EBOOK

This book is an up-to-date and comprehensive collection of reviews on various aspects of epigenetic gene silencing in plants. Research on this topic has undergone explosive growth during the past decade and has revealed novel features of gene regulation and plant defense responses that also apply to animals and fungi. Gene silencing is relevant for agricultural biotechnology because stable expression of transgenes is required for the successful commercialization of genetically engineered crops. The reviews have been written by distinguished authors who have made significant contributions to plant gene silencing research. This volume supersedes other books on gene silencing by focussing on plant systems, where many pioneering experiments have been performed, and by including the latest developments from top laboratories. The book is geared toward advanced students of genetics and plant sciences as well as applied and basic research scientists who work with transgenic organisms and epigenetic regulation of gene expression.

RNAi
RNAi

Author: Gregory J. Hannon

Publisher: CSHL Press

Published: 2003

Total Pages: 550

ISBN-13: 9780879696412

DOWNLOAD EBOOK

In the past three years, the use of double-stranded RNA to silence gene activity has become widely and rapidly adopted. RNA interference is highly specific and remarkably potent, and it acts on cells and tissues far removed from the site of introduction. The principles behind RNAi are just being uncovered, but this laboratory technique has been applied effectively in a wide variety of animal and plant species. Variations on RNAi are revolutionizing many approaches to experimental biology, complementing traditional genetic technologies with a quicker and less expensive way of mimicking the effects of mutations both in cell cultures and in living animals. Recent advances in the use of RNAi to engineer heritable silencing in mammals, to alter stem cells for organ reconstitution, and to alter the course of disease in model systems indicate that RNAi may have a future in disease therapy. Written by pioneers in this new field and edited by Gregory Hannon, one of its leading figures, RNAi: A Guide to Gene Silencing presents the principles of RNAi and reliable protocols for its laboratory use in Caenorhabditis elegans, Drosophila, plants, avian embryos, mammalian cells, mouse oocytes, and more. This important and unique book is an essential laboratory resource for scientists studying gene regulation and for all experimental biologists interested in the emerging practical applications of RNAi.

RNAi Technology
RNAi Technology

Author: R. K. Gaur

Publisher: CRC Press

Published: 2016-04-19

Total Pages: 388

ISBN-13: 1439873259

DOWNLOAD EBOOK

RNAi technology is used for large-scale screens that systematically shut down each gene in the cell, which can help identify the components necessary for a particular cellular process or an event such as cell division. Exploitation of the pathway is also a promising tool in biotechnology and medicine. Introducing new technology in the study of RNA

RNA Interference in Practice
RNA Interference in Practice

Author: Ute Schepers

Publisher: John Wiley & Sons

Published: 2006-03-06

Total Pages: 336

ISBN-13: 3527604375

DOWNLOAD EBOOK

This hands-on guide to RNA interference brings the power of targeted gene silencing to any laboratory with the basic equipment for handling nucleic acids. In easy-to-follow, step-by-step protocols you will learn * how RNAi works in worms, flies and mammals, * how to design the most efficient RNAi constructs, * how to achieve transient, stable and conditional RNAi in cell cultures, * how to determine the efficiency of an RNAi experiment, * and how to use RNAi for gene therapy. All the protocols have been thoroughly tested in the author's own laboratory, and she provides examples of successful experiments and troubleshooting hints to help in establishing your own successful RNAi experiments. Also includes a list of suppliers for RNAi reagents and equipment as well as a glossary of terms.

RNA Silencing
RNA Silencing

Author: Gordon Carmichael

Publisher: Springer Science & Business Media

Published: 2008-02-04

Total Pages: 343

ISBN-13: 1592599354

DOWNLOAD EBOOK

A collection of readily reproducible methods for the design, preparation, and use of RNAs for silencing gene expression in cells and organisms. The techniques range widely and include methods addressing the biochemical aspects of the silencing machinery, RNA silencing in non-mammalian organisms, and the in vivo delivery of siRNAs and silencing vectors. There are also techniques for designing, preparing, and using RNAs to silence gene expression, for fine-tuning regulation by targeting specific isoforms of a given gene, and for the study and use of microRNAs. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and Molecular Phytopathology
Comprehensive and Molecular Phytopathology

Author: Yuri Dyakov

Publisher: Elsevier

Published: 2007-01-09

Total Pages: 497

ISBN-13: 0080469337

DOWNLOAD EBOOK

This book offers a collection of information on successive steps of molecular 'dialogue' between plants and pathogens. It additionally presents data that reflects intrinsic logic of plant-parasite interactions. New findings discussed include: host and non-host resistance, specific and nonspecific elicitors, elicitors and suppressors, and plant and animal immunity. This book enables the reader to understand how to promote or prevent disease development, and allows them to systematize their own ideas of plant-pathogen interactions.* Offers a more extensive scope of the problem as compared to other books in the market* Presents data to allow consideration of host-parasite relationships in dynamics and reveals interrelations between pathogenicity and resistance factors* Discusses beneficial plant-microbe interactions and practical aspects of molecular investigations of plant-parasite relationships* Compares historical study of common and specific features of plant immunity with animal immunity

RNA Silencing
RNA Silencing

Author: Esra Galun

Publisher: World Scientific

Published: 2005

Total Pages: 469

ISBN-13: 9812562060

DOWNLOAD EBOOK

This book focuses on an emerging, central issue in molecular genetics and the development of eukaryotes: the control of gene expression by small species of RNA. As an exciting new field of endeavor, it is the first book by a single author to deal comprehensively with RNA silencing.The book provides the historical background of the field preceding the seminal work by Fire and associates in 1998 on the impact of small double-stranded RNA on the expression of nematode genes, which is considered the beginning of RNA silencing research. RNA silencing is described in a wide range of plants and animals including protozoa, simple metazoa, insects, non-mammalian vertebrates, and mammals. In each case the experimental results are provided with the accompanying background and with illustrations. There is also an appendix on the prospective use of RNA silencing in gene therapy, which is intended as a guide for investigators wishing to explore this possibility.

RNA Interference
RNA Interference

Author: Dr. Tim Doran

Publisher: CABI

Published: 2009

Total Pages: 269

ISBN-13: 1845934105

DOWNLOAD EBOOK

This methods manual provides an introduction to RNA interference, the theory behind its many applications, and specific protocols for RNAi, in organisms from plants and C.elegans to Drosophila and mammals. There are also chapters covering small hairpin RNAs and viral-induced gene silencing.

Plant Virology
Plant Virology

Author: Roger Hull

Publisher: Academic Press

Published: 2013-10-31

Total Pages: 1119

ISBN-13: 0123848725

DOWNLOAD EBOOK

The seminal text Plant Virology is now in its fifth edition. It has been 10 years since the publication of the fourth edition, during which there has been an explosion of conceptual and factual advances. The fifth edition of Plant Virology updates and revises many details of the previous edition while retaining the important earlier results that constitute the field's conceptual foundation. Revamped art, along with fully updated references and increased focus on molecular biology, transgenic resistance, aphid transmission, and new, cutting-edge topics, bring the volume up to date and maintain its value as an essential reference for researchers and students in the field. - Thumbnail sketches of each genera and family groups - Genome maps of all genera for which they are known - Genetic engineered resistance strategies for virus disease control - Latest understanding of virus interactions with plants, including gene silencing - Interactions between viruses and insect, fungal, and nematode vectors - Contains over 300 full-color illustrations

RNA Infrastructure and Networks
RNA Infrastructure and Networks

Author: Lesley J. Collins

Publisher: Springer Science & Business Media

Published: 2011-09-15

Total Pages: 297

ISBN-13: 1461403324

DOWNLOAD EBOOK

RNAs form complexes with proteins and other RNAs. The RNA‐infrastructure represents the spatiotemporal interaction of these proteins and RNAs in a cell‐wide network. RNA Infrastructure and Networks brings together these ideas to illustrate the scope of RNA‐based biology, and how connecting RNA mechanisms is a powerful tool to investigate regulatory pathways. This book is but a taste of the wide range of RNA‐based mechanisms that connect in the RNA infrastructure.