Recombinant Proteins from Plants
Recombinant Proteins from Plants

Author: Charles Cunningham

Publisher: Springer Science & Business Media

Published: 2008-02-05

Total Pages: 309

ISBN-13: 1603272607

DOWNLOAD EBOOK

Recombinant Proteins from Plants is one of the most exciting and fastest developing areas in biology. The latest molecular techniques are being applied to the exploitation of plants as novel expression systems for the p- duction and overproduction of heterologous and native proteins. Transgenic plant technology is currently used in three broad areas: the expression of - combinant proteins to improve crop quality by increasing disease/pest res- tance or increasing tolerance to stress, optimizing plant productivity and yield by the genetic manipulation of metabolic pathways, and the large-scale co- effective production of recombinant proteins for use as specialist industrial or therapeutic biomolecules. The intention of Recombinant Proteins from Plants is to provide c- prehensive and detailed protocols covering all the latest molecular approaches. Because the production oftransgenic plants has become routine in many la- ratories, coverage is also given to some of the more "classical" approaches to the separation, analysis, and characterization of recombinant proteins. The book also includes areas of research that we believe will become increasingly important in the near future: efficient transformation of monocots with Agrobacterium optimizing the stability of recombinant proteins, and a section highlighting the immunotherapeutic potential of plant-expressed proteins.

Recombinant Proteins in Plants
Recombinant Proteins in Plants

Author: Stefan Schillberg

Publisher: Humana

Published: 2022-05-27

Total Pages: 346

ISBN-13: 9781071622407

DOWNLOAD EBOOK

This volume provided methods and protocols on recombinant protein production in different plant systems, downstream processing, and strategies to optimize protein expression. Chapters guide readers through recombinant protein production in important plant systems, protein recovery and purification, different strategies to optimise productivity, cloning and fusion protein approaches, and the regulation and freedom to operate analysis of plant-produced proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Recombinant Proteins in Plants: Methods and Protocols aims to be useful to newcomers and experienced researchers interested in expanding their expertise in the field of plant-based protein production. Chapters 6, 8 and 17 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Commercial Plant-Produced Recombinant Protein Products
Commercial Plant-Produced Recombinant Protein Products

Author: John A. Howard

Publisher: Springer

Published: 2014-09-01

Total Pages: 282

ISBN-13: 3662438364

DOWNLOAD EBOOK

Attention has recently turned to using plants as hosts for the production of commercially important proteins. The twelve case studies in this volume present successful strategies for using plants to produce industrial and pharmaceutical proteins and vaccine antigens. They examine in detail projects that have commercial potential or products that have already been commercialized, illustrating the advantages that plants offer over bacterial, fungal or animal cell-culture hosts. There are many indications that plant protein production marks the beginning of a new paradigm for the commercial production of proteins that, over the next decade, will expand dramatically.

RECOMBINANT PROTEINS FROM PLAN
RECOMBINANT PROTEINS FROM PLAN

Author: Jacqueline MacDonald

Publisher: Methods in Molecular Biology

Published: 2016-11-27

Total Pages: 310

ISBN-13: 9781493950041

DOWNLOAD EBOOK

This volume provides up-to-date scientific achievements from the world s top researchers. "Recombinant Proteins from Plants: Methods and Protocols, Second Edition" guides readers through protocolsfor use with a variety of plant expression systems. Various aspects of production are covered including vector selection and cloning; product improvements for stability, glycosylation, and antibiotic-free selection; extraction and scale-up; and analysis of transgenic plants and their recombinant proteins. Written for the "Methods in Molecular Biology" series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical," ""Recombinant Proteins from Plants: Methods and Protocols, Second Edition" is an ideal reference for those who are interested in plant molecular biology and molecular farming."

Recombinant Protein Expression: Eukaryotic hosts
Recombinant Protein Expression: Eukaryotic hosts

Author:

Publisher: Academic Press

Published: 2021-11-04

Total Pages: 384

ISBN-13: 0323907385

DOWNLOAD EBOOK

Recombinant Protein Expression, Part B, Volume 660 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on Multiplexed analysis protein: Protein interactions of polypeptides translated in Leishmania cell-free system, MultiBac system and its applications, performance and recent, Production of antibodies in Shuffle, Designing hybrid-promoter architectures by engineering cis-acting DNA sites to enhance transcription in yeast, Designing hybrid-promoter architectures by engineering cis-acting DNA sites to deregulate transcription in yeast, Antibody or protein-based vaccine production in plants, Cell-free protein synthesis, Plant-based expression of biologic drugs, and much more. Additional sections cover the Use of native mass spectrometry to guide detergent-based rescue of non-native oligomerization by recombinant proteins, Advancing overexpression and purification of recombinant proteins by pilot optimization through tandem affinity-buffer exchange chromatography online with native mass spectrometry, Method for High-Efficiency Fed-batch cultures of recombinant Escherichia coli, Method to transfer Chinese hamster ovary (CHO) shake flask experiments to the ambr® 250, and Expression of recombinant antibodies in Leishmania tarentolae. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology serial Updated release includes the latest information on Recombinant Protein Expression

Plants as Factories for Protein Production
Plants as Factories for Protein Production

Author: Elizabeth E. Hood

Publisher: Springer Science & Business Media

Published: 2013-03-09

Total Pages: 232

ISBN-13: 9401726930

DOWNLOAD EBOOK

This exciting volume Plants as Factories for Protein Production, edited by Drs. Elizabeth E. Hood and John A. Howard, contains chapters by experts in the field of molecular farming. The information within addresses the leading plant systems for recombinant protein production, as well as the progress being made in leading product categories - human pharmaceuticals, animal health, and industrial enzymes. More importantly, the book includes chapters that address the hot topics of production, containment, regulatory, and legal aspects that are quickly coming to the forefront of the industry. This most timely text is appropriate for graduate students and post-doctoral fellows, as well as being a key text for faculty, pharmaceutical producers, and industrial enzyme users.

Proteins: Sustainable Source, Processing and Applications
Proteins: Sustainable Source, Processing and Applications

Author: Charis M. Galanakis

Publisher: Academic Press

Published: 2019-05-30

Total Pages: 358

ISBN-13: 012817286X

DOWNLOAD EBOOK

Proteins: Sustainable Source, Processing and Applications addresses sustainable proteins, with an emphasis on proteins of animal origin, plant-based and insect proteins, microalgal single cell proteins, extraction, production, the stability and bioengineering of proteins, food applications (e.g. encapsulation, films and coatings), consumer behavior and sustainable consumption. Written in a scientific manner to meet the needs of chemists, food scientists, technologists, new product developers and academics, this book addresses the health effects and properties of proteins, highlights sustainable sources, processes and consumption models, and analyzes the potentiality of already commercialized processes and products. This book is an integral resource that supports the current applications of proteins in the food industry, along with those that are currently under development. Supports the current applications of proteins in the food industry, along with those that are under development Connects the properties and health effects of proteins with sustainable sources, recovery procedures, stability and encapsulation Explores industrial applications that are affected by aforementioned aspects

Molecular Farming in Plants: Recent Advances and Future Prospects
Molecular Farming in Plants: Recent Advances and Future Prospects

Author: Aiming Wang

Publisher: Springer Science & Business Media

Published: 2011-10-14

Total Pages: 284

ISBN-13: 9400722176

DOWNLOAD EBOOK

Molecular farming in plants is a relatively young subject of sciences. As plants can offer an inexpensive and convenient platform for the large-scale production of recombinant proteins with various functions, the driven force from the giant market for recombinant protein pharmaceuticals and industrial enzymes makes this subject grow and advance very quickly. To summarize recent advances, current challenges and future directions in molecular farming, international authorities were invited to write this book for researchers, teachers and students who are interested in this subject. This book, with the focus on the most advanced cutting-edge breakthroughs, covers all the essential aspects of the field of molecular farming in plants: from expression technologies to downstream processing, from products to safety issues, and from current advances and holdups to future developments.