Protein Biotechnology

Protein Biotechnology

Author: Felix Franks

Publisher: Springer Science & Business Media

Published: 2008-02-22

Total Pages: 594

ISBN-13: 1592594387

DOWNLOAD EBOOK

Proteins are the servants of life. They occur in all com- nent parts of living organisms and are staggering in their fu- tional variety, despite their chemical similarity. Even the simplest single-cell organism contains a thousand different p- teins, fulfilling a wide range of life-supporting roles. Additions to the total number of known proteins are being made on an increasing scale through the discovery of mutant strains or their production by genetic manipulation. The total international protein literature could fill a medi- sized building and is growing at an ever-increasing rate. The reader might be forgiven for asking whether yet another book on proteins, their properties, and functions can serve a useful purpose. An explanation of the origin of this book may serve as justification. The authors form the tutorial team for an int- sive postexperience course on protein characterization or- nized by the Center for Professional Advancement, East Brunswick, New Jersey, an educational foundation. The course was first mounted in Amsterdam in 1982 and has since been repeated several times, in both Amsterdam and the US, with participants from North America and most European countries. In a predecessor to this book, emphasis was placed on the role of protein isolation in the food industry, because at the time this reflected the interests of most of the participants at the course. Today, isolated proteins for food use are extracted from yeasts, fungal sources, legumes, oilseeds, cereals, and leaves.


Fundamentals of Protein Biotechnology

Fundamentals of Protein Biotechnology

Author: Stein

Publisher: CRC Press

Published: 1990-08-31

Total Pages: 336

ISBN-13: 9780824783464

DOWNLOAD EBOOK

Fills a gap between the existing studies of proteins, which tend to be highly technical and geared toward the practicing protein chemist, and biochemistry textbooks, which focus on general principles. Scientists cover a dozen topics by presenting fundamental principles, an overview, and the practica


Protein Hydrolysates in Biotechnology

Protein Hydrolysates in Biotechnology

Author: Vijai K. Pasupuleti

Publisher: Springer Science & Business Media

Published: 2010-08-28

Total Pages: 237

ISBN-13: 1402066740

DOWNLOAD EBOOK

Protein hydrolysates, otherwise commonly known as peptones or peptides, are used in a wide variety of products in fermentation and biotechnology industries. The term “peptone” was first introduced in 1880 by Nagelli for growing bacterial cultures. However, later it was discovered that peptones derived from the partial digestion of proteins would furnish organic nitrogen in readily available form. Ever since, p- tones, which are commonly known as protein hydrolysates, have been used not only for growth of microbial cultures, but also as nitrogen source in commercial fermen- tions using animal cells and recombinant microorganisms for the production of value added products such as therapeutic proteins, hormones, vaccines, etc. Today, the characterization, screening and manufacturing of protein hyd- lysates has become more sophisticated, with the introduction of reliable analytical instrumentation, high throughput screening techniques coupled with statistical design approaches, novel enzymes and efficient downstream processing equipment. This has enabled the introduction of custom-built products for specialized appli- tions in diverse fields of fermentation and biotechnology, such as the following. 1. Protein hydrolysates are used as much more than a simple nitrogen source. For example, the productivities of several therapeutic drugs made by animal cells and recombinant microorganisms have been markedly increased by use of p- tein hydrolysates. This is extremely important when capacities are limited. 2. Protein hydrolysates are employed in the manufacturing of vaccines by ferm- tation processes and also used as vaccine stabilizers.


Proteins

Proteins

Author: Gary Walsh

Publisher: John Wiley & Sons

Published: 2014-03-13

Total Pages: 447

ISBN-13: 1118851471

DOWNLOAD EBOOK

Proteins Biochemistry and Biotechnology 2e is a definitive source of information for all those interested in protein science, and particularly the commercial production and isolation of specific proteins, and their subsequent utilization for applied purposes in industry and medicine. Fully updated throughout with new or fundamentally revised sections on proteomics as, bioinformatics, protein glycosylation and engineering, well as sections detailing advances in upstream processing and newer protein applications such as enzyme-based biofuel production this new edition has an increased focus on biochemistry to ensure the balance between biochemisty and biotechnology, enhanced with numerous case studies. This second edition is an invaluable text for undergraduates of biochemistry and biotechnology but will also be relevant to students of microbiology, molecular biology, bioinformatics and any branch of the biomedical sciences who require a broad overview of the various medical, diagnostic and industrial uses of proteins. • Provides a comprehensive overview of all aspects of protein biochemisty and protein biotechnology • Includes numerous case studies • Increased focus on protein biochemistry to ensure balance between biochemisty and biotechnology • Includes new section focusing on proteomics as well as sections detailing protein function and enzyme-based biofuel production "With the potential of a standard reference source on the topic, any molecular biotechnologist will profit greatly from having this excellent book. " (Engineering in Life Sciences, 2004; Vol 5; No. 5) “Few texts would be considered competitors, and none compare favorably." (Biochemistry and Molecular Education, July/August 2002) "...The book is well written, making it informative and easy to read..." (The Biochemist, June 2002)


Protein Engineering

Protein Engineering

Author: Huimin Zhao

Publisher: John Wiley & Sons

Published: 2021-08-23

Total Pages: 41

ISBN-13: 3527344705

DOWNLOAD EBOOK

A one-stop reference that reviews protein design strategies to applications in industrial and medical biotechnology Protein Engineering: Tools and Applications is a comprehensive resource that offers a systematic and comprehensive review of the most recent advances in the field, and contains detailed information on the methodologies and strategies behind these approaches. The authors—noted experts on the topic—explore the distinctive advantages and disadvantages of the presented methodologies and strategies in a targeted and focused manner that allows for the adaptation and implementation of the strategies for new applications. The book contains information on the directed evolution, rational design, and semi-rational design of proteins and offers a review of the most recent applications in industrial and medical biotechnology. This important book: Covers technologies and methodologies used in protein engineering Includes the strategies behind the approaches, designed to help with the adaptation and implementation of these strategies for new applications Offers a comprehensive and thorough treatment of protein engineering from primary strategies to applications in industrial and medical biotechnology Presents cutting edge advances in the continuously evolving field of protein engineering Written for students and professionals of bioengineering, biotechnology, biochemistry, Protein Engineering: Tools and Applications offers an essential resource to the design strategies in protein engineering and reviews recent applications.


Food Safety of Proteins in Agricultural Biotechnology

Food Safety of Proteins in Agricultural Biotechnology

Author: Bruce G. Hammond

Publisher: CRC Press

Published: 2007-11-28

Total Pages: 318

ISBN-13: 1420005731

DOWNLOAD EBOOK

With contributions from internationally recognized experts, Food Safety of Proteins in Agricultural Biotechnology comprehensively addresses how toxicology testing of proteins should be accomplished and how protein safety assessments should be carried out. Beginning with a background on protein biology, the book delineates the fundamental difference


Biotechnology of Plasma Proteins

Biotechnology of Plasma Proteins

Author: Roger L. Lundblad

Publisher: CRC Press

Published: 2012-08-08

Total Pages: 463

ISBN-13: 1439850267

DOWNLOAD EBOOK

The fractionation of human blood plasma can be considered to be a mature industry, with the basic technology, alcohol fractionation, dating back at least to the 1940s. Many of the products described in the current work have been approved biologics since the 1950s. The information gathered from the development of plasma proteins has proved vital to the development of recombinant therapeutic proteins. Discussing the role of plasma proteins in current biotechnology, Biotechnology of Plasma Proteins describes the protein composition of human plasma, the fractionation of plasma to obtain therapeutic proteins, and the analysis of these products. It delineates the path from plasma products to recombinant products, and highlights products from albumin, intravenous immunoglobins, and coagulation. It offers a comprehensive review of current techniques for the analysis of proteins including electrophoresis, chromatography, spectrophotometry, mass spectrometry, and updates not published since 1975. Key Topics Protein Composition of Plasma Proteomic methods for plasma protein analysis Plasma protein biomarkers Validation of biomarkers Assays for plasma biomarkers Methods for the Analysis of Protein Products Assay development and validation Electrophoresis Chromatography Immunoassay Mass spectrometry Raman spectroscopy Plasma Fractionation: Historical and Modern Methods Development of Cohn alcohol fractionation Industrial methods Development of chromatographic methods Plasma Protein Products of Therapeutic Value Albumin Intravenous immunoglobulin Coagulation products Growth factors Wound management


Mammalian Cell Biotechnology in Protein Production

Mammalian Cell Biotechnology in Protein Production

Author: Hansjörg Hauser

Publisher: Walter de Gruyter

Published: 2011-07-13

Total Pages: 513

ISBN-13: 3110809281

DOWNLOAD EBOOK

„Hauser und Wagner haben die neuen Möglichkeiten der Mammalian Cell Biology sehr anregend dargestellt.“ Prof. Dr. Hans Fritz, Ludwig-Maximilians-Universität München


CE in Biotechnology: Practical Applications for Protein and Peptide Analyses

CE in Biotechnology: Practical Applications for Protein and Peptide Analyses

Author: Anthony B. Chen

Publisher: Springer Science & Business Media

Published: 2013-04-17

Total Pages: 103

ISBN-13: 3322830217

DOWNLOAD EBOOK

The goal of this book is to show recent developments in the CE analysis of protein pharmaceuticals. It is devoted completely to practical concerns to strengthen the use of CE within the biotechnology industry, highlighting the uses of CE in various areas of product development including formulation studies, process development, product characterization and validated lot release and stability testing.


Development and Manufacture of Protein Pharmaceuticals

Development and Manufacture of Protein Pharmaceuticals

Author: Steve L. Nail

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 479

ISBN-13: 1461505496

DOWNLOAD EBOOK

In this era of biotechnology there have been many books covering the fundamentals of recombinant DNA technology and protein chemistry. However, not many sources are available for the pharmaceutical develop ment scientist and other personnel responsible for the commercialization of the finished dosage forms of these new biopharmaceuticals and other products from biotechnology. This text will help to fill this gap. Once active biopharmaceutical molecules are candidates for clinical trial investigation and subsequent commercialization, a number of other activities must take place while research and development on these molecules continues. The active ingredient itself must be formulated into a finished dosage form that can be conveniently used by health care professionals and patients. Properties of the biopharmaceutical molecule must be clearly understood so that the appropriate finished product formulation can be developed. Finished product formulation development includes not only the chemical formulation, but also the packaging system, the manufacturing process, and appropriate control strategies to assure such good manufacturing practice attributes as safety, identity, strength, purity, and quality.