This is an ideal practical manual of biochemistry for MBBS students. It includes flowcharts, diagrams and colour pictures for clear visualization and understanding of the topics. Formulation of working reagents has been described along with each experiment. The manual includes viva-voce questions as well as information on biomedical waste segregations and disposal.
Most lab manuals assume a high level of knowledge among biochemistry students, as well as a large amount of experience combining knowledge from separate scientific disciplines. Biochemistry in the Lab: A Manual for Undergraduates expects little more than basic chemistry. It explains procedures clearly, as well as giving a clear explanation of the theoretical reason for those steps. Key Features: Presents a comprehensive approach to modern biochemistry laboratory teaching, together with a complete experimental experience Includes chemical biology as its foundation, teaching readers experimental methods specific to the field Provides instructor experiments that are easy to prepare and execute, at comparatively low cost Supersedes existing, older texts with information that is adjusted to modern experimental biochemistry Is written by an expert in the field This textbook presents a foundational approach to modern biochemistry laboratory teaching together with a complete experimental experience, from protein purification and characterization to advanced analytical techniques. It has modules to help instructors present the techniques used in a time critical manner, as well as several modules to study protein chemistry, including gel techniques, enzymology, crystal growth, unfolding studies, and fluorescence. It proceeds from the simplest and most important techniques to the most difficult and specialized ones. It offers instructors experiments that are easy to prepare and execute, at comparatively low cost.
Biochemistry laboratory manual for undergraduates – an inquiry based approach by Gerczei and Pattison is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology while incorporating the blossoming field of bioinformatics. The novelty of this manual is the incorporation of a student-driven real real-life research project into the undergraduate curriculum. Since students test their own mutant design, even the most experienced students remain engaged with the process, while the less experienced ones get their first taste of biochemistry research. Inclusion of a research project does not entail a limitation: this manual includes all classic biochemistry techniques such as HPLC or enzyme kinetics and is complete with numerous problem sets relating to each topic.
This book will serve as a practical manual for undergraduate students in MBBS. Related clinical concepts will also be useful in the preparation of postgraduate entrance exams. This book will serve as a practical manual for undergraduate students in MBBS. Related clinical concepts will also to useful in the preparation of Post-graduate entrance exams.
1. General General Instructions Laboratory Equipments and Procedures Buffers and pH Quality Control 2. Analysis of Urine and Cerebrospinal Fluid Normal Urine: Characteristics and Analysis Abnormal Constituents of Urine Screening of Urine for Inborn Errors of Metabolism and the Use of Paper Chromatography Composition of Cerebrospinal Fluid 3. Quantitative Experiments Principles of Colorimetry Principles of Spectrophotometer Estimation of Glucose Glucose Tolerance Test Estimation of Serum and Urine Creatinine: Creatinine Clearance Estimation of Urea Serum Proteins--Albumin: Globulin Ratio Serum Total Cholesterol Lipid Profile Serum Calcium and Phosphorus Serum Bilirubin Serum Transaminases Serum Alkaline Phosphatase Serum Uric Acid 4. Equipments And Procedures Point of Care Testing pH Meter Paper Chromatography and Thin Layer Chromatography Protein Electrophoresis Polyacrylamide Gel Electrophoresis Electrolyte Analysis Arterial Blood Gas Analyzer Enzyme-Linked Immunosorbent Assay Immunodiffusion Autoanalyzer DNA Isolation from Blood/Tissues 5. Function Tests Gastric Function Tests and Analysis of Gastric Juice Pancreatic Function Tests Liver Function Tests Analysis of Bile Renal Function Tests Thyroid Function Tests Adrenal Function Tests 6. Food and Energy Energy Content of Food and Glycemic Index Fats in Food 7. Clinical Case Studies Basis and Rationale of Biochemical Tests Done in Certain Clinical Conditions 8. Objective Structured Practical Examination Objective Structured Practical Examination 9. Reagents Reagent Preparation 10. Normal Values Normal Values Worksheet
Based on BHMS syllabus by CCH.This book deals with the basic knowledge of practical biochemistry but also its application in actual clinical practice.Creates an abiding interest in the practical aspects of the subject.
Though many practical books are available in the market but this Laboratory Manual of Microbiology, Biochemistry and Molecular Biology is an unique combination of protocols that covers maximum (about 80%) of the practicals of various Indian universities for UG and PG courses in Bioscience, Biotechnology, Microbiology, Biochemistry and Biochemical Engineering.
