Mass Spectrometry-Based Chemical Proteomics
Mass Spectrometry-Based Chemical Proteomics

Author: W. Andy Tao

Publisher: John Wiley & Sons

Published: 2019-07-10

Total Pages: 449

ISBN-13: 1118970217

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PROVIDES STRATEGIES AND CONCEPTS FOR UNDERSTANDING CHEMICAL PROTEOMICS, AND ANALYZING PROTEIN FUNCTIONS, MODIFICATIONS, AND INTERACTIONS—EMPHASIZING MASS SPECTROMETRY THROUGHOUT Covering mass spectrometry for chemical proteomics, this book helps readers understand analytical strategies behind protein functions, their modifications and interactions, and applications in drug discovery. It provides a basic overview and presents concepts in chemical proteomics through three angles: Strategies, Technical Advances, and Applications. Chapters cover those many technical advances and applications in drug discovery, from target identification to validation and potential treatments. The first section of Mass Spectrometry-Based Chemical Proteomics starts by reviewing basic methods and recent advances in mass spectrometry for proteomics, including shotgun proteomics, quantitative proteomics, and data analyses. The next section covers a variety of techniques and strategies coupling chemical probes to MS-based proteomics to provide functional insights into the proteome. In the last section, it focuses on using chemical strategies to study protein post-translational modifications and high-order structures. Summarizes chemical proteomics, up-to-date concepts, analysis, and target validation Covers fundamentals and strategies, including the profiling of enzyme activities and protein-drug interactions Explains technical advances in the field and describes on shotgun proteomics, quantitative proteomics, and corresponding methods of software and database usage for proteomics Includes a wide variety of applications in drug discovery, from kinase inhibitors and intracellular drug targets to the chemoproteomics analysis of natural products Addresses an important tool in small molecule drug discovery, appealing to both academia and the pharmaceutical industry Mass Spectrometry-Based Chemical Proteomics is an excellent source of information for readers in both academia and industry in a variety of fields, including pharmaceutical sciences, drug discovery, molecular biology, bioinformatics, and analytical sciences.

ADP-Ribose Transfer Reactions
ADP-Ribose Transfer Reactions

Author: Myron K. Jacobson

Publisher: Springer

Published: 2012-01-11

Total Pages: 0

ISBN-13: 9781461585091

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Current interest in NAD (Nicotinamide adenine dinucleotide) in biological systems focuses on its role in ADP-ribose transfer reactions. These appear to be fundamentally involved in the regulation of many physiological processes. The contributions in this monograph thus represent the range of research in the very active investigation of niacin metabolism. The major topics covered are: ??? - Enzymology of ADP-Ribosylation - ADP-Ribosylation and Chromatin Function - Carcinogenesis and Differentiation - NAD Metabolism and Chemotherapy - ADP-Ribosylation and Signal Transduction - Molecular Genetic Approaches to ADP-Ribosylation

DNA Damage, DNA Repair and Disease
DNA Damage, DNA Repair and Disease

Author: Miral Dizdaroglu

Publisher: Royal Society of Chemistry

Published: 2020-11-11

Total Pages: 509

ISBN-13: 1839162562

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The DNA of all organisms is constantly being damaged by endogenous and exogenous sources. Oxygen metabolism generates reactive species that can damage DNA, proteins and other organic compounds in living cells. Exogenous sources include ionizing and ultraviolet radiations, carcinogenic compounds and environmental toxins among others. The discovery of multiple DNA lesions and DNA repair mechanisms showed the involvement of DNA damage and DNA repair in the pathogenesis of many human diseases, most notably cancer. These books provide a comprehensive overview of the interdisciplinary area of DNA damage and DNA repair, and their relevance to disease pathology. Edited by recognised leaders in the field, this two-volume set is an appealing resource to a variety of readers including chemists, chemical biologists, geneticists, cancer researchers and drug discovery scientists.

Fundamentals of Chromatin
Fundamentals of Chromatin

Author: Jerry L. Workman

Publisher: Springer Science & Business Media

Published: 2013-12-04

Total Pages: 594

ISBN-13: 1461486246

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​​​​​​​​​​​​​While there has been an increasing number of books on various aspects of epigenetics, there has been a gap over the years in books that provide a comprehensive understanding of the fundamentals of chromatin. ​Chromatin is the combination of DNA and proteins that make up the genetic material of chromosomes. Its primary function is to package DNA to fit into the cell, to strengthen the DNA to prevent damage, to allow mitosis and meiosis, and to control the expression of genes and DNA replication. The audience for this book is mainly newly established scientists ​and graduate students. Rather than going into the more specific areas of recent research on chromatin the chapters in this book give a strong, updated groundwork about the topic. Some the fundamentals that this book will cover include the structure of chromatin and biochemistry and the enzyme complexes that manage it.

Mitochondrial Medicine
Mitochondrial Medicine

Author: Volkmar Weissig

Publisher: Springer

Published: 2021-06-01

Total Pages: 459

ISBN-13: 9781071612651

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This second edition offers 88 chapters divided among three volumes providing the most comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. Volume II guides readers through chapters on mitochondrial dysfunction, functional’ mitochondria, mitochondrial retrograde, mitochondrial dNTP pool quantification, mitochondrial ADP-ribosylation, blue-native gel approach, 3D optical cryo-imaging method, mitochondrial ATP and ROS production, protocol for untargeted metabolomic analysis, and methods for analysis of nitrotyrosine-containing proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Mitochondrial Medicine, Second Edition, Volume 2: Assessing Mitochondria aims to be a comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine.

Poly(ADP-Ribosyl)ation
Poly(ADP-Ribosyl)ation

Author: Alexander Bürkle

Publisher: Springer Science & Business Media

Published: 2008-01-11

Total Pages: 260

ISBN-13: 0387360050

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This is the most comprehensive, up-to-date reference on this post-translational modification of proteins, which is intimately linked with DNA repair, maintenance of genomic stability, transcriptional regulation, cell death and a variety of other cellular phenomena as well as with a variety of pathophysiological conditions, including ischemia-reperfusion damage, Parkinson’s disease, Type I diabetes mellitus, hemorrhagic and septic shock and other inflammatory conditions. Richly illustrated, it offers 19 chapters written by international experts.

ADP-Ribosylation of Proteins
ADP-Ribosylation of Proteins

Author: Felix R. Althaus

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 248

ISBN-13: 3642830773

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In 1966, a paper entitled "On the formation of a novel adenylylic compound by enzymatic extracts of liver nuclei" from Paul Mandel's laboratory in Strasbourg, France, planted the seed for a rapidly growing new field of biological research focusing on ADP-ribosylation reactions. The development of this field over the past 2 decades reflects very much a modern trend of biological research. As more detailed knowledge accumulates, enigmatic phenomena turn into concepts which create their own enigmata. This process tends to favor the development of multiple, seemingly disconnected, research lines until simplicity emerges from chaos and unifying concepts substitute for controversy. It appears that the field of ADP-ribosylation reactions has not yet attained this latter stage. For example, with the identification of two different classes of ADP-ribosylation reactions, i.e., mono-ADP-ribosyla tion and poly-ADP-ribosylation reactions, the field split very early into two separate branches of research. With the present volume, we have divided the task of reviewing these two classes of ADP ribosylation accordingly, although their coexistence in eukaryotes may involve a closer functional linkage than hitherto recognized.

ADP-Ribosylation Reactions
ADP-Ribosylation Reactions

Author: Osamu Hayaishi

Publisher: Elsevier

Published: 2012-12-02

Total Pages: 725

ISBN-13: 0323142990

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ADP-Ribosylation Reactions: Biology and Medicine deals with the biochemical and physiological aspects of poly(ADP-ribose) and ADP-ribosylation of proteins. Topics covered range from pyridine nucleotide metabolism and ADP-ribosylation to the structure and properties of poly(ADP-ribose), along with acceptor proteins of poly(ADP-ribose). ADP-ribosyl protein linkages and poly(ADP-ribose) synthetase are also discussed. Comprised of 39 chapters, this book begins with a historical background on the discovery of poly(ADP-ribose) and the significance of poly- and mono(ADP-ribosyl)ation reactions in molecular biology. The next section explores the role of ADP-ribosylation in NAD metabolism, paying particular attention to poly(ADP-ribose) synthetase-DNA interaction and the link between chromatin structure and poly(ADP-ribosyl)ation. Subsequent chapters focus on subfractions and subcellular distribution of mono(ADP-ribosyl) proteins in eukaryotic cells; polyadenylylation and ADP-ribosylation of reovirus proteins; poly(ADP-ribose) synthesis in plants; and immunohistochemistry of poly(ADP-ribose). The final chapter analyzes the ADP-ribosylation activity of toxin A and exoenzyme S in Pseudomonas aeruginosa. This monograph is written for students, practitioners, and researchers in fields such as molecular biology, medical chemistry, and biochemistry.

ADP-Ribosylation Reactions
ADP-Ribosylation Reactions

Author: R. Alvarez-Gonzalez

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 156

ISBN-13: 1441987401

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This special issue of Molecular and Cellular Biochemistry contains twenty-two selected research papers and reviews from a total of one hundred and ten presentations given at the 12th International Symposium on ADP-ribosylation Reactions: From Bacterial Pathogenesis to Cancer, held in Cancun, Mexico, May 10-14, 1997. The Symposium was hosted by the Sociedad Mexicana de Bioquimica and was sponsored by the University of North Texas Health Science Center, Fort Worth, TX, USA. This volume provides a state-of-the-art source of information for basic scientists and clinicians who are interested in the molecular, biochemical, and cellular aspects of protein-(ADP-ribose) transfer reactions in human health and disease.

ADP-Ribosylation: Metabolic Effects and Regulatory Functions
ADP-Ribosylation: Metabolic Effects and Regulatory Functions

Author: Joel Moss

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 243

ISBN-13: 1461526140

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Considering the current interest in cellular regulation and intracellular signalling systems, it is surprising that the contribution of ADP-ribosylation reactions to the modulation of a variety of specific cell processes, in parallel with other post-translational modifications such as phosphorylation, has not been generally recognized. While it is not feasible to cover all aspects of ADP-ribosylation, the thirty-one articles contained in this volume provide a valuable overview of recent progress in the field within the context of cell control mechanisms. For the convenience of the reader, the various topics have been grouped into several sections: (a) poly(ADP-ribosyl)ation; (b) mono-ADP-ribosylation; (c) toxin mono-ADP-ribosylation; (d) inhibitors and activators; (e) protein modification with ADP-ribose and its analogues; and (f) non-modification forms of ADP-ribose. The contents of the individual chapters reflect the ideas of the contributors, many of whom have spent their careers attempting to resolve the biological functions of ADP-ribosylation. We hope that this publication will serve as a useful reference for those investigators that are new to the area as well as those who are actively studying ADP-ribosylation.