Test No. 474: Mammalian Erythrocyte Micronucleus Test
Test No. 474: Mammalian Erythrocyte Micronucleus Test

Author: OECD

Publisher: OECD Publishing

Published: 2014-09-26

Total Pages: 21

ISBN-13: 9264224297

DOWNLOAD EBOOK

The mammalian in vivo micronucleus test detects damage induced by the test substance to the chromosomes or the mitotic apparatus of erythroblasts, by analysis of erythrocytes as sampled in bone marrow and/or peripheral blood cells of animals.

OECD Guidelines for the Testing of Chemicals, Section 4 Test No. 482: Genetic Toxicology: DNA Damage and Repair, Unscheduled DNA Synthesis in Mammalian Cells in vitro
OECD Guidelines for the Testing of Chemicals, Section 4 Test No. 482: Genetic Toxicology: DNA Damage and Repair, Unscheduled DNA Synthesis in Mammalian Cells in vitro

Author: OECD

Publisher: OECD Publishing

Published: 1986-10-23

Total Pages: 7

ISBN-13: 926407144X

DOWNLOAD EBOOK

The Test Guideline for Unscheduled DNA Synthesis (UDS) in mammalian cells in vitro describes procedures utilizing primary cultures, human lymphocytes or established cell lines, to detect DNA repair synthesis after excision and removal of a stretch ...

OECD Guidelines for the Testing of Chemicals, Section 4 Test No. 470: Mammalian Erythrocyte Pig-a Gene Mutation Assay
OECD Guidelines for the Testing of Chemicals, Section 4 Test No. 470: Mammalian Erythrocyte Pig-a Gene Mutation Assay

Author: OECD

Publisher: OECD Publishing

Published: 2022-06-30

Total Pages: 37

ISBN-13: 9264366067

DOWNLOAD EBOOK

This Test Guideline describes an in vivo erythrocyte Pig-a gene mutation assay (hereafter called the Pig-a assay) which uses an endogenous mammalian gene, the phosphatidylinositol glycan class A gene (Pig-a), as a reporter of somatic-cell gene mutation. In vivo gene mutation tests, such as the Pig-a assay, are especially relevant for assessing mutagenicity because physiological factors, such as absorption of the test chemical from the site of exposure, distribution of the test chemical throughout the test system via systemic circulation, and in vivo metabolism and DNA repair processes, all contribute to the mutagenic responses.