Nucleic Acid Techniques in Bacterial Systematics

Nucleic Acid Techniques in Bacterial Systematics

Author: Erko Stackebrandt

Publisher:

Published: 1991-04-19

Total Pages: 370

ISBN-13:

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One of a series whose aim is to identify specialist areas in microbiology and to provide up-to-date methodological information for laboratory microbiologists, active researchers and graduate students. This volume addresses nucleic acid techniques in bacterial systematics.


Chemical Methods in Bacterial Systematics

Chemical Methods in Bacterial Systematics

Author: Society for Applied Bacteriology

Publisher:

Published: 1985

Total Pages: 440

ISBN-13:

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Current chemosystematics owes much to the availability of highly sensitive analytical techniques. Many of these involve a quantitative approach, utilising computers and a variety of statistical techniques. This book contains up-to-date procedures on chemical methods being developed and used for the classification and identification of bacteria, including medically and economically significant ones. Detailed protocols are given on how valuable taxonomic data can be derived from analyses of nucleic acids, proteins, lipids and whole-organisms. Of general interest to microbiologists, the volume should also be an essential reference to those actively engaged in diagnostic bacteriology or microbial systematics. FROM THE PREFACE: Chemical methods not only are well established in bacterial systematics but are also rapidly changing our views on how bacteria should be classified and identified. Chemotaxonomy, which owes much to the availability of new and highly sensitive analytical techniques, can be used to establish relationships between bacteria at all levels in the taxonomic hierarchy. However, many of the new approaches are designed for the identification of bacteria and some are quantitative, employing computers and a variety of statistical techniques. This book contains detailed procedures on many of the chemical methods currently being used for the classification and identification of bacteria. As such, it will be of value generally to microbiologists and is an essential reference for those actively engaged in diagnostic bacteriology.


Molecular Identification, Systematics, and Population Structure of Prokaryotes

Molecular Identification, Systematics, and Population Structure of Prokaryotes

Author: Erko Stackebrandt

Publisher: Springer

Published: 2013-01-02

Total Pages: 0

ISBN-13: 9783642062148

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Systematic biology has a far wider application than merely the provision of a reliable classification scheme for new strains. With the framework of the hierarchic system stabilizing, genomes, noncoding regions, and genes and their products can now be evaluated in an evolutionary context. This book summarizes recent developments in the molecular characterization of cultured and as-yet uncultured prokaryotes, emphasizing the strengths and weaknesses of individual approaches. The chapters of the book are compiled to stimulate students to enter the field of bacterial diversity, presenting a broad spectrum of fascinating multifaceted disciplines that illuminate the paths to ecosystem functioning, communication within communities, symbiosis, life in extreme environments, astrobiology, and more.


Bacterial Diversity and Systematics

Bacterial Diversity and Systematics

Author: F.G. Priest

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 329

ISBN-13: 1461518695

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Bacterial taxonomy as a specialized discipline is practised by a minority but the applications of taxonomy are important to most, if not all microbiologists. It is the implementation of taxonomic ideas and practises which gives rise to identification and typing systems, procedures for the analysis and characterization of biodiversity, hypotheses about the evolution of micro-organisms, and improved procedures for the isolation and implementation of bacteria in biotechnological processes. Without taxonomic theory providing a sound basis to these many facets of microbiology there would be severe problems faced by many scientists working with micro-organisms. Taxonomy comprises three sequential but independent processes; classification, nomenclature and identification. The first two stages are the prime concern of the specialist taxonomist but the third stage should result in identification schemes of value to all microbiologists. As the classification and identification of micro-organisms improves, largely due to the introduction of new technologies, so does its contribution to the subject as a whole. It therefore seemed timely to hold a conference in the autumn of 1993 devoted to microbial identification. Such a topic could not be addressed without some reference to the enabling discipline of classification, but the principal aims were to assess improvements in identification and typing and how these were benefiting microbiological topics ranging from ecological and biotechnological studies of extremophilic bacteria to the use of pyrolysis mass spectrometry in epidemiology. The meeting, which was held in Granada, Spain, was supported by FEMS (FEMS Symposium No.


Molecular Microbial Ecology Manual

Molecular Microbial Ecology Manual

Author: Antoon D. L. Akkermans

Publisher: Springer

Published: 2014-01-13

Total Pages: 266

ISBN-13: 9400902158

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For a long time microbial ecology has been developed as a distinct field within Ecology. In spite of the important role of microorganisms in the environment, this group of 'invisible' organisms remained unaccessable to other ecologists. Detection and identification of microorganisms remain largely dependent on isolation techniques and characterisation of pure cul tures. We now realise that only a minor fraction of the microbial com munity can be cultivated. As a result of the introduction of molecular methods, microbes can now be detected and identified at the DNA/RNA level in their natural environment. This has opened a new field in ecology: Molecular Microbial Ecology. In the present manual we aim to introduce the microbial ecologist to a selected number of current molecular techniques that are relevant in micro bial ecology. The first edition of the manual contains 33 chapters and an equal number of additional chapters will be added this year. Since the field of molecular ecology is in a continuous progress, we aim to update and extend the Manual regularly and will invite anyone to depo sit their new protocols in full detail in the next edition of this Manual. We hope this book finds its place where it was born: at the lab bench! Antoon D.L. Akkermans, Jan Dirk van Elsas and Frans J. de Bruijn March 1995 Molecular Microbial Ecology Manual 1.3.6: 1-8, 1996. © 1996 Kluwer Academic Publishers.


Modern Bacterial Taxonomy

Modern Bacterial Taxonomy

Author: F. G. Priest

Publisher: Springer Science & Business Media

Published: 1993-11-30

Total Pages: 244

ISBN-13: 9780412461200

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This second edition of Modern Bacterial Taxonomy has been completely revised and expanded to include detailed coverage of molecular systematics including relevant aspects of nucleic acid sequences, the construction of phylogenetic trees, typing of bacteria by restriction fragment length polymorphisms, DNA hybridization probes and the use of the polymerase chain reaction in bacterial systematics.


Applied Microbial Systematics

Applied Microbial Systematics

Author: Fergus Priest

Publisher: Springer Science & Business Media

Published: 2000-11-30

Total Pages: 508

ISBN-13: 9780792365181

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Modern approaches to microbial classification and identification, particularly those based on nucleic acid analysis, have raised the awareness and interest of microbiologists in systematics during the past decade. The extended scope of the subject has revolutionized microbial ecology with the demonstration of uncultivable microorganisms as a major component of the biosphere and evolution, with the ribosomal RNA phylogenetic tree as the basis of current classifications. However, advances in microbial systematics have also had enormous impact on other, diverse aspects of microbiology such as animal pathogenicity, plant-microbe interactions and relationships with food. In this book, we survey and discuss in depth the contribution of modern taxonomic approaches to our understanding of the microbiology of these various systems. The book does not concentrate on methods - these have been well reported elsewhere - instead it provides a unique insight into the application and value of modern systematics in diverse branches of microbiology. It will be of value to microbiologists at both research and technical levels who need to appreciate the range of organisms with which they work and the diversity within them. It will also be of value to teachers and students of microbiology courses who want to understand how systematics can enhance microbiology beyond the routine of classification, nomenclature, and identification.


Techniques in Molecular Systematics and Evolution

Techniques in Molecular Systematics and Evolution

Author: Rob DeSalle

Publisher: Birkhäuser

Published: 2013-12-01

Total Pages: 414

ISBN-13: 3034881258

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The amount of information that can be obtained by using molecular techniques in evolution, systematics and ecology has increased exponentially over the last ten years. The need for more rapid and efficient methods of data acquisition and analysis is growing accordingly. This manual presents some of the most important techniques for data acquisition developed over the last years. The choice and justification of data analysis techniques is also an important and critical aspect of modern phylogenetic and evolutionary analysis and so a considerable part of this volume addresses this important subject. The book is mainly written for students and researchers from evolutionary biology in search for methods to acquire data, but also from molecular biology who might be looking for information on how data are analyzed in an evolutionary context. To aid the user, information on web-located sites is included wherever possible. Approaches that will push the amount of information which systematics will gather in the


The Pangenome

The Pangenome

Author: Hervé Tettelin

Publisher: Springer Nature

Published: 2020-04-30

Total Pages: 311

ISBN-13: 3030382818

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This open access book offers the first comprehensive account of the pan-genome concept and its manifold implications. The realization that the genetic repertoire of a biological species always encompasses more than the genome of each individual is one of the earliest examples of big data in biology that opened biology to the unbounded. The study of genetic variation observed within a species challenges existing views and has profound consequences for our understanding of the fundamental mechanisms underpinning bacterial biology and evolution. The underlying rationale extends well beyond the initial prokaryotic focus to all kingdoms of life and evolves into similar concepts for metagenomes, phenomes and epigenomes. The book’s respective chapters address a range of topics, from the serendipitous emergence of the pan-genome concept and its impacts on the fields of microbiology, vaccinology and antimicrobial resistance, to the study of microbial communities, bioinformatic applications and mathematical models that tie in with complex systems and economic theory. Given its scope, the book will appeal to a broad readership interested in population dynamics, evolutionary biology and genomics.