Rice seed health and quarantine; The rice plant and its environment; Equipment; Samples and sampling; dry seed inspection; Fungi; Bacteria; Nematodes; Viruses and mycoplasmalike organisms; Field inspection; Seed treatment; Weed seed; Insect pests; Fungal pathogens; Bacterial pathogens; Nematode pest; Organisms causing grain discoloration and damage.
This practical guide covers the commonly used detection methods for seed-transmitted viruses and viroids that affect both tropical and temperate crops. It contains 25 complete step-by-step procedures for biological, serological and molecular techniques to detect and identify such viruses. Combining helpful practical notes with more detailed explanations of the principles behind the techniques, the book describes the general characteristics of seed-transmitted viral diseases and discusses outlines for the organization and interpretation of seed health assays. The techniques reviewed are also applicable to non-seed-transmitted viral agents.
From prehistoric times man has had a pecial s relationship with seed plants - as a source of food, materials for tools, buildings, clothing and pharmaceuticals, and for ornamenting his surroundings for his own delight (probably in that chronological order which, incidentally, also gives some indication ofthe priorities oflife). Today man's most important staple foods are derived directly from seeds as they have been since neolithic times. (It is a sobering thought, as Harlan* has pointed out, that nothing significant has been added to his diet since then. ) From those times he must have learned to collect, conserve and cultivate seeds; and the accumulated experience has been handed down. This book then is part of an ancient tradition, for here we are still primarily concerned with these skills. Seeds are plant propagules comprised of embryos in which growth has been suspended, usually supplied with their own food reserves and protected by special covering layers. Typically they are relatively dry structures compared with other plant tissues and, in this condi tion) they are resistant to the ravages of time and their environment. But resistant is a relative tenn and seeds do deteriorate: the type, the extent and the rapidity of the deterioration, and the factors which control it are important to agronomists, horticulturalists, plant breeders, seedsmen, seed analysts, and those concerned with the conservation of genetic resources.
