Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols
Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols

Author: Andreas Marx

Publisher: Humana Press

Published: 2008-04-03

Total Pages: 266

ISBN-13: 9781588297006

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From probe design to applications in clinical settings, this book provides a diverse set of instructive examples, guided by experts in the field who offer easy-to-follow experimentals. The book first offers an introduction to the basic principles of fluorescence and then describes applications of fluorogenic probes in real-time PCR, which currently is the gold standard for quantitative DNA and RNA analysis. Coverage extends the potential of realtime as well as advocates simplifications of the probe technologies. It also presents a new simplified molecular beacon design, EasyBeacons, and demonstrates the utility in DNA methylation profiling.

Hedgehog Signaling Protocols
Hedgehog Signaling Protocols

Author: Jamila I. Horabin

Publisher: Springer Science & Business Media

Published: 2007-09-13

Total Pages: 267

ISBN-13: 158829692X

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In both vertebrates and invertebrates, Hedgehog signaling regulates the development of the tissues and structures of the body. In addition, many cancers and diseases arise as a result of misregulation of the pathway, which makes it an incredibly important system to understand. This volume examines various methods used in the analysis of the Hedgehog signaling pathway, bringing together different lines of analyses into one accessible and comprehensive text. This volume provides a single source for investigators, presenting several different experimental approaches, which are all varied and broad in nature, as well as procedures for a variety of different model systems. Researchers will find this volume to be an invaluble tool as they work towards deeper understanding of the underlying molecular events that the Hedgehog signaling pathway regulates.

Neuroinformatics
Neuroinformatics

Author: Chiquito J. Crasto

Publisher: Springer Science & Business Media

Published: 2007-11-29

Total Pages: 419

ISBN-13: 1588297209

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Neuroinformatics presents cutting-edge techniques for the synergistic study of neuroinformatics. The book facilitates the efforts of discovering neuroscience through the sharing of data and the use of computational models. It demonstrates the use of neuroinformatic components as a mechanism for understanding complex disorders. It contains detailed explanations, advantages, and disadvantages of traditional and non-invasive imaging methods.

Hematopoietic Stem Cell Protocols
Hematopoietic Stem Cell Protocols

Author: Kevin D. Bunting

Publisher: Springer Science & Business Media

Published: 2008-01-31

Total Pages: 306

ISBN-13: 158829868X

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This revised edition provides up-to-date protocols developed in the HSC field. A team of leading researchers supply this volume with in-depth, readily reproducible methods for effective characterization of HSC and their developmental potential. The book provides detailed flow cytometry protocols for thorough analysis of enriched HSC populations, and offers a variety of transplantation approaches to measure HSC function in vivo. This is a much needed technical resource in the critically important field of stem cell investigation.

Telomerase Inhibition
Telomerase Inhibition

Author: Lucy Andrews

Publisher: Springer Science & Business Media

Published: 2007-11-29

Total Pages: 197

ISBN-13: 1588296830

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This volume presents a compendium of the most recent and advanced methods applied to the rapidly expanding field of telomerase inhibition. The techniques described provide the researcher with a diverse and comprehensive set of tools for the study of telomerase inhibition. The volume is aimed at biochemists, molecular biologists, cancer researchers, and geneticists.

Nucleic Acid Detection
Nucleic Acid Detection

Author: Dmitry M. Kolpashchikov

Publisher: Humana Press

Published: 2013-09-13

Total Pages: 0

ISBN-13: 9781627035347

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In Nucleic Acid Chemistry: Methods and Protocols, expert researches in the field detail techniques and approaches for the detection of DNA and RNA. These techniques include the recovery of trace amounts of DNA for amplification and analysis, new qPCR chemistries, new application of isothermal amplification techniques, assays with visual or electric signals for point-of-care diagnostics, improvement of fluorescent in situ hybridization, and new signal amplification techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Nucleic Acid Chemistry: Methods and Protocols seeks to aid scientists in the further study of detection for DNA and RNA.

Clinical Proteomics
Clinical Proteomics

Author: Antonia Vlahou

Publisher: Springer Science & Business Media

Published: 2008-01-18

Total Pages: 401

ISBN-13: 158829837X

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In this book, a select group of researchers has contributed their state-of-the-art methodologies on protein profiling and identification of disease biomarkers in tissues, microdissected cells and body fluids. The book integrates biochemistry, pathology, analytical technology, bioinformatics, and proteome informatics. Experimental approaches are thoroughly detailed and explained through a step-by-step instructional format that ensures successful results.

Organelle Proteomics
Organelle Proteomics

Author: Delphine Pflieger

Publisher: Springer Science & Business Media

Published: 2008-02-19

Total Pages: 423

ISBN-13: 1588297799

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This is the first book to examine organelle proteomics in depth. It begins by introducing the different analytical strategies developed and successfully utilized to study organelle proteomes, and detailing the use of multidimensional liquid chromatography coupled to tandem mass spectrometry for peptide sample analysis. Detailed protocols are provided and a section is devoted to methods enabling a global estimate of the reliability of the protein list assigned to an organelle.

Gene Function Analysis
Gene Function Analysis

Author: Michael F. Ochs

Publisher: Springer Science & Business Media

Published: 2007-08-23

Total Pages: 703

ISBN-13: 1588297349

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With the advent of high-throughput technologies following completion of the human genome project and similar projects, the number of genes of interest has expanded and the traditional methods for gene function analysis cannot achieve the throughput necessary for large-scale exploration. This book brings together a number of recently developed techniques for looking at gene function, including computational, biochemical and biological methods and protocols.

Alcohol
Alcohol

Author: Laura E. Nagy

Publisher: Springer Science & Business Media

Published: 2008-03-07

Total Pages: 418

ISBN-13: 1588299066

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This book examines the pleiotropic effects of ethanol in animal and cell culture models through a collection of detailed procedures written by experts in the field. Sections present clearly defined models of ethanol exposure, recent advances in the development of specific methodologies to mimic the impact of ethanol metabolism in cultured cells, and methodologies to investigate a variety of cells and tissues that are known to be disrupted by ethanol, amongst other topics.