Modern Methods of Steroid Analysis
Modern Methods of Steroid Analysis

Author: Erich Heftmann

Publisher: Elsevier

Published: 2012-12-02

Total Pages: 558

ISBN-13: 0323146449

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Modern Methods of Steroid Analysis reviews modern methods of steroid analysis such as liquid column chromatography, mass spectrometry, and gas chromatography. Topics covered include qualitative and quantitative analysis of plant sterols by gas-liquid chromatography; Raman spectroscopy of steroids; nuclear magnetic resonance; and applications of lanthanide shift reagents. A computerized method for rapid comparison and retrieval of infrared spectral data is also described. This volume is comprised of 22 chapters and begins with an analysis of hormonal steroids using liquid column chromatography, followed by a discussion on the separation of insect molting hormones using high-pressure liquid chromatography. The reader is methodically introduced to the application of gradient elution and thin-layer chromatography to the analysis of corticosteroids and 17-ketosteroids, as well as some aspects of mass spectrometry in steroid analysis. Subsequent chapters explore other methods such as optical rotatory dispersion and circular dichroism, along with radioisotope techniques. In particular, the radioimmunoassay of plasma steroid hormones and plasma aldosterone is considered. This book should be of interest to biochemists.

Targeted Biomarker Quantitation by LC-MS
Targeted Biomarker Quantitation by LC-MS

Author: Naidong Weng

Publisher: John Wiley & Sons

Published: 2017-07-31

Total Pages: 397

ISBN-13: 1119103061

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The first book to offer a blueprint for overcoming the challenges to successfully quantifying biomarkers in living organisms The demand among scientists and clinicians for targeted quantitation experiments has experienced explosive growth in recent years. While there are a few books dedicated to bioanalysis and biomarkers in general, until now there were none devoted exclusively to addressing critical issues surrounding this area of intense research. Target Biomarker Quantitation by LC-MS provides a detailed blueprint for quantifying biomarkers in biological systems. It uses numerous real-world cases to exemplify key concepts, all of which were carefully selected and presented so as to allow the concepts they embody to be easily expanded to future applications, including new biomarker development. Target Biomarker Quantitation by LC-MS primarily focuses on the assay establishment for biomarker quantitation—a critical issue rarely treated in depth. It offers comprehensive coverage of three core areas of biomarker assay establishment: the relationship between the measured biomarkers and their intended usage; contemporary regulatory requirements for biomarker assays (a thorough understanding of which is essential to producing a successful and defendable submission); and the technical challenges of analyzing biomarkers produced inside a living organism or cell. Covers the theory of and applications for state-of-the-art mass spectrometry and chromatography and their applications in biomarker analysis Features real-life examples illustrating the challenges involved in target biomarker quantitation and the innovative approaches which have been used to overcome those challenges Addresses potential obstacles to obtain effective biomarker level and data interpretation, such as specificity establishment and sample collection Outlines a tiered approach and fit-for-purpose assay protocol for target biomarker quantitation Highlights the current state of the biomarker regulatory environment and protocol standards Target Biomarker Quantitation by LC-MS is a valuable resource for bioanalytical scientists, drug metabolism and pharmacokinetics scientists, clinical scientists, analytical chemists, and others for whom biomarker quantitation is an important tool of the trade. It also functions as an excellent text for graduate courses in pharmaceutical, biochemistry and chemistry.

LC-MS in Drug Bioanalysis
LC-MS in Drug Bioanalysis

Author: Q. Alan Xu

Publisher: Springer Science & Business Media

Published: 2012-07-15

Total Pages: 476

ISBN-13: 1461438284

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Clinical pharmacology plays an important role in today’s medicine. Due to the high sensitivity, selectivity, and affordability of a mass spectrometer (MS), the high performance liquid chromatography – mass spectrometry (LC-MS) analytical technique is widely used in the determination of drugs in human biological matrixes for clinical pharmacology. Specifically, LC-MS is used to analyze: anticancer drugs antidementia drugs antidepressant drugs antiepileptic drugs antifundal drug antimicrobial drugs antipsychotic drugs antiretroviral drugs anxiolytic/hypnotic drugs cardiac drugs drugs for addiction immunosuppressant drugs mood stabilizer drugs This book will primarily cover the various methods of validation for LC-MS techniques and applications used in modern clinical pharmacology.

Tandem Mass Spectrometry of Lipids
Tandem Mass Spectrometry of Lipids

Author: Robert C Murphy

Publisher: Royal Society of Chemistry

Published: 2014-12-02

Total Pages: 295

ISBN-13: 1782626352

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The emerging field of lipidomics has been made possible because of advances in mass spectrometry, and in particular tandem mass spectrometry of lipid ions generated by electrospray ionization. The ability to carry out basic biochemical studies of lipids using electrospray ionization is predicated upon understanding the behaviour of lipid derived ions following collision induced decomposition and mechanisms of product ion formation. During the past 20 years, a wealth of information has been generated about lipid molecules that are now analysed by mass spectrometry, however there is no central source where one can obtain basic information about how these very diverse biomolecules behave following collisional activation. This book brings together, in one volume, this information so that investigators considering using tandem mass spectrometry to structurally characterize lipids or to quantitate their occurrence in a biological matrix, will have a convenient source to review mechanism of decomposition reactions related to the diversity of lipid structures. A separate chapter is devoted to each of seven major lipid classes including fatty acids, eicosanoids and bioactive lipid mediators, fatty acyl esters and amides, glycerol esters, glycerophospholipids, sphingolipids, and steroids. Mechanistic details are provided for understanding the pathways of formation of major product ions and ions used for structural characterization. In most cases specific ancillary information has been critical to understand the pathways, including isotope labeling and high resolution analysis of precursor and product ions. For a few specific examples such data is missing and pathways are proposed as a means to initiate further mass spectral experiments to prove or disprove pathway hypotheses. While this work largely centres on the lipid biochemistry of animal (mammalian) systems, general principles can be taken from the specific examples and applied to lipid biochemistry found in plants, fungi, prokaryotes and archeal organisms.

Diagnosis and Management of Ovarian Disorders
Diagnosis and Management of Ovarian Disorders

Author: Albert Altchek

Publisher: Elsevier

Published: 2003-09-04

Total Pages: 595

ISBN-13: 008049451X

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This updated second edition of Diagnosis and Management of Ovarian Disorders provides thorough, yet succinct insight into the ever-changing realm of ovarian disorders. It presents a novel multidisciplinary approach to the subject as described by clinicians, surgeons, pathologists, basic scientists and related medical researchers. Topics covered include reproductive technology, early diagnosis of ovarian cancer, and management of menopause among others. The breadth of information provided by this book will appeal to clinicians and researchers involved in the study and treatment of ovarian disorders.KEY FEATURES* Includes updated information on early diagnosis of ovarian cancer* Reviews new diagnostic techniques for ovarian disorders* Discusses latest information on reproductive technology* Presents translational treatment linking laboratory research with clinical medicine

Oxford Textbook of Endocrinology and Diabetes
Oxford Textbook of Endocrinology and Diabetes

Author: John A.H. Wass

Publisher: Oxford University Press, USA

Published: 2011-07-28

Total Pages: 2158

ISBN-13: 0199235295

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Now in its second edition, the Oxford Textbook of Endocrinology and Diabetes is a fully comprehensive, evidence-based, and highly-valued reference work combining basic science with clinical guidance, and providing first rate advice on diagnosis and treatment.

Chromatography and Isolation of Insect Hormones and Pheromones
Chromatography and Isolation of Insect Hormones and Pheromones

Author: A.R. McCaffery

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 372

ISBN-13: 1468480626

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The connection between the study of insects. their development. behaviour and biochemistry. and chromatography is perhaps not immediately obvious. However. this connection exists and it is of fundamental importance to our understanding of many areas of insect physiology. Insects range in size from small to minute and consequently the amounts of hormones or pheromones they produce are equally minute. Ultimately any attempt at understanding the processes which control development, social behaviour or the biochemistry of insects requires some means of isolating the tiny quantities of the hormones and pheromones responsible in sufficient quantity and purity for identification. The ability to devise novel techniques to separate these materials from frequently complex biological mixtures including precursors and metabolites and to devise detection systems for them is vital. Methods for the quantification of these substances at different stages in the life cycle. or in response to environmental change or stress. are then essential. Chromatography. both as a means for isolation and as a method for quantitative analysis. has "been an essential tool in these studies. This volume represents the outcome of a joint international symposium organized by the Chromatographic and Royal Entomological Societies at the University of Reading between the 21st and 23rd March 1989 aimed specifically at discussing the chromatography and isolation of insect hormones. pheromones and related substances.The papers presented at that meeting. and collected together here. covered many aspects of the subject including the chromatography of juvenile hormones. ecdysteroids. peptides. pheromones and semio chemicals.

Analysis of Sterols
Analysis of Sterols

Author: J. Goad

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 453

ISBN-13: 9400914474

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Knowledge is of two kinds. We know a subject ourselves, or we know where we can find information on it Samuel Johnson, 18 April, 1775* Sterols are among the most studied groups of natural products with interest commencing in the 19th century and running to the present. Investigations have embraced the refinement of separation procedures, the development of new analytical techniques and instrumentation for structure elucidation, the unravelling of biosynthetic mechanisms, the determination of the physiological functions of sterols, and the role they play in health and disease. In the past 20-30 years interest in the medical implications of sterol biochemistry, studies on the sterols of plants, algae and fungi, and the identification of the many unusual sterols from marine organisms have proceeded in parallel and somewhat independently. Although the motiva tion and goals for the various lines of investigation have differed widely the researchers working in each of these areas have contributed a wealth of knowledge to the literature relating to the analysis of sterols and many diverse new sterols have been discovered. We conceived this book as a modest attempt to bring together some of this literature in the hope that it may be helpful to newcomers to sterol research. We had originally intended to produce a 'handbook' outlining in detail the protocols to be followed for sterol extraction, chromatography, NMR analysis, etc. in order to identify the components of a sterol mixture.