Digital Microscopy
Digital Microscopy

Author: Greenfield Sluder

Publisher: Elsevier

Published: 2007-04-26

Total Pages: 626

ISBN-13: 0080544347

DOWNLOAD EBOOK

The previous edition of this book marked the shift in technology from video to digital camera use with microscope use in biological science. This new edition presents some of the optical fundamentals needed to provide a quality image to the digital camera. Specifically, it covers the fundamental geometric optics of finite- and infinity-corrected microscopes, develops the concepts of physical optics and Abbe's theory of image formation, presents the principles of Kohler illumination, and finally reviews the fundamentals of fluorescence and fluorescence microscopy. The second group of chapters deals with digital and video fundamentals: how digital and video cameras work, how to coordinate cameras with microscopes, how to deal with digital data, the fundamentals of image processing, and low light level cameras. The third group of chapters address some specialized areas of microscopy that allow sophisticated measurements of events in living cells that are below the optical limits of resolution. - Expands coverage to include discussion of confocal microscopy not found in the previous edition - Includes "traps and pitfalls" as well as laboratory exercises to help illustrate methods

Cellular Electron Microscopy
Cellular Electron Microscopy

Author: J. Richard McIntosh

Publisher: Elsevier

Published: 2011-09-02

Total Pages: 878

ISBN-13: 0080475035

DOWNLOAD EBOOK

Recent advances in the imaging technique electron microscopy (EM) have improved the method, making it more reliable and rewarding, particularly in its description of three-dimensional detail. Cellular Electron Microscopy will help biologists from many disciplines understand modern EM and the value it might bring to their own work. The book's five sections deal with all major issues in EM of cells: specimen preparation, imaging in 3-D, imaging and understanding frozen-hydrated samples, labeling macromolecules, and analyzing EM data. Each chapter was written by scientists who are among the best in their field, and some chapters provide multiple points of view on the issues they discuss. Each section of the book is preceded by an introduction, which should help newcomers understand the subject. The book shows why many biologists believe that modern EM will forge the link between light microscopy of live cells and atomic resolution studies of isolated macromolecules, helping us toward the goal of an atomic resolution understanding of living systems. - Updates the numerous technological innovations that have improved the capabilities of electron microscopy - Provides timely coverage of the subject given the significant rise in the number of biologists using light microscopy to answer their questions and the natural limitations of this kind of imaging - Chapters include a balance of "how to", "so what" and "where next", providing the reader with both practical information, which is necessary to use these methods, and a sense of where the field is going

Quantitative Imaging in Cell Biology
Quantitative Imaging in Cell Biology

Author:

Publisher: Academic Press

Published: 2014-06-25

Total Pages: 609

ISBN-13: 0124202012

DOWNLOAD EBOOK

This new volume, number 123, of Methods in Cell Biology looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications. The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detector impact the quality of quantitative data, and include step-by-step protocols for capturing and analyzing quantitative images. Common quantitative applications, including co-localization, ratiometric imaging, and counting molecules, are covered in detail. Practical chapters cover topics critical to getting the most out of your imaging system, from microscope maintenance to creating standardized samples for measuring resolution. Later chapters cover recent advances in quantitative imaging techniques, including super-resolution and light sheet microscopy. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material

Methods in Cell Biology
Methods in Cell Biology

Author:

Publisher: Academic Press

Published: 2020-05-16

Total Pages: 196

ISBN-13: 012820009X

DOWNLOAD EBOOK

Methods in Cell Biology, Volume 158, the latest release in this series, highlights new advances in the field, with this release covering How to orient cells in micro-cavities for high resolution imaging of cytokinesis and lumen formation, A body-on-a-chip (BOC) system for studying gut-liver interaction, Manipulating cultured mammalian cells for mitosis research, Live-cell FLIM-FRET using a commercially available system, A comparative analysis of methods to measure kinetochore-microtubule attachment stability, A workflow for visualizing human cancer biopsies using large-format electron microscopy, Isolation of stage-specific germ cells using facs in drosophila germarium, Computational analysis of filament polymerization dynamics in cytoskeletal networks, and more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Cell Biology series - Updated release includes the latest information in this area of study

Introduction to Electron Microscopy for Biologists
Introduction to Electron Microscopy for Biologists

Author:

Publisher: Academic Press

Published: 2008-10-22

Total Pages: 562

ISBN-13: 008088816X

DOWNLOAD EBOOK

This volume demonstrates how cellular and associated electron microscopy contributes to knowledge about biological structural information, primarily at the nanometer level. It presents how EM approaches complement both conventional structural biology (at the high end, angstrom level of resolution) and digital light microscopy (at the low end, 100-200 nanometers). Basic techniques in transmission and scanning electron microscopy Detailed chapters on how to use electron microscopy when dealing with specific cellular structures, such as the nucleus, cell membrane, and cytoskeleton Discussion on electron microscopy of viruses and virus-cell interactions

A Practical Guide to the Study of Calcium in Living Cells
A Practical Guide to the Study of Calcium in Living Cells

Author: Richard Nuccitelli

Publisher: Academic Press

Published: 1994-03-10

Total Pages: 418

ISBN-13: 9780125641418

DOWNLOAD EBOOK

A Practical Guide to the Study of Calcium in Living Cells describes popular techniques along with helpful do's and don't's and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research needs. This book is designed for laboratory use by graduate students, technicians, and researchers in many disciplines, ranging from molecular to cellular levels of investigation. Describes techniques for detection of [Ca2+]I: Ca2+ - sensitive microelectrodes Fluorescent dyes Luminescent proteins Includes techniques for perturbing intracellular Ca2+ Covers detailed methodology plus problems and pitfalls of each technique Contains a practical guide to preparing Ca2+ buffers with an easy-to-use computer program Color plates illustrate techniques such as Confocal ratio-imaging Use of aequorin

Computational Methods in Cell Biology
Computational Methods in Cell Biology

Author:

Publisher: Academic Press

Published: 2012-05-31

Total Pages: 427

ISBN-13: 0123884217

DOWNLOAD EBOOK

Computational methods are playing an ever increasing role in cell biology. This volume of Methods in Cell Biology focuses on Computational Methods in Cell Biology and consists of two parts: (1) data extraction and analysis to distill models and mechanisms, and (2) developing and simulating models to make predictions and testable hypotheses. - Focuses on computational methods in cell biology - Split into 2 parts--data extraction and analysis to distill models and mechanisms, and developing and simulating models to make predictions and testable hypotheses - Emphasizes the intimate and necessary connection with interpreting experimental data and proposing the next hypothesis and experiment

Advanced Micropipette Techniques for Cell Physiology
Advanced Micropipette Techniques for Cell Physiology

Author: Kenneth T. Brown

Publisher:

Published: 1986

Total Pages: 320

ISBN-13:

DOWNLOAD EBOOK

IBRO Handbook Series: Methods in the Neurosciences, Volume 9 General Editor: A. D. Smith Advanced Micropipette Techniques for Cell Physiology Kenneth T. Brown and Dale G. Flaming With this Handbook Series, IBRO aims to fill a major gap in the world literature. The neuroscientist needs to be able to turn to whichever specialized method is most suited to the problem he is currently studying. Methods in the Neurosciences will help to provide expert advice on exactly how to carry out the experiments, on what difficulties can occur and on the limitations of the method. Fine glass micropipettes are extensively used in intra and extra-cellular physiology as a means of recording electrical activity in cells and as a way of injecting a variety of substances for experimental purposes. For the last 12 years the authors of this book have treated micropipette techniques as a research field in itself, and here present for the first time their theory of how micropipette tips are formed, their methods of reducing tip size, and the implications of their work for research on small cells of all kinds, especially cells within the central nervous system. The book not only incorporates this new work but reviews and analyses existing publications on micropipette methodology, including patch-clamping, in order to present as complete an account as possible of how micropipettes can be used efficiently and effectively in a wide variety of experimental situations.

Biochemistry and Cell Culture
Biochemistry and Cell Culture

Author: P. Michael Conn

Publisher:

Published: 2012

Total Pages: 505

ISBN-13: 9781283836968

DOWNLOAD EBOOK

Cell biology spans among the widest diversity of methods in the biological sciences. From physical chemistry to microscopy, cells have given up with secrets only when the questions are asked in the right way! This new volume of Methods in Cell Biology covers laboratory methods in cell biology, and includes methods that are among the most important and elucidating in the discipline, such as transfection, cell enrichment and magnetic batch separation. Covers the most important laboratory methods in cell biology Chapters written by experts in their fields.