Meta-topolin: A Growth Regulator for Plant Biotechnology and Agriculture

Meta-topolin: A Growth Regulator for Plant Biotechnology and Agriculture

Author: Naseem Ahmad

Publisher: Springer Nature

Published: 2021-05-02

Total Pages: 339

ISBN-13: 981159046X

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Plant tissue culture (PTC) technology has gained unassailable success for its various commercial and research applications in plant sciences. Plant growth regulators (PGRs) are an essential part of any plant tissue culture intervention for propagation or modification of plants. A wide range of PGRs are available, including aromatic compounds that show cytokinin activities, promote cell division and micro-propagation, viz. kinetin, N6-benzyladenine and topolins. Topolins are naturally occurring aromatic compounds that have gained popularity as an effective alternative for other frequently used cytokinins in in vitro culture of plants. Among them, meta-topolin [6-(3-hydroxybenzlyamino) purine] is the most popular and its use in plant tissue culture has amplified swiftly. During the last few decades, there have been numerous reports highlighting the effectiveness of meta-topolin in micropropagation and alleviation of various physiological disorders, rooting and acclimatization of tissue culture raised plants.


Thidiazuron: From Urea Derivative to Plant Growth Regulator

Thidiazuron: From Urea Derivative to Plant Growth Regulator

Author: Naseem Ahmad

Publisher: Springer

Published: 2018-03-23

Total Pages: 489

ISBN-13: 9811080046

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Plant biotechnology is a most interesting branch for academicians and researchers in recent past. Now days, it becomes a very useful tool in agriculture and medicine and is regarded as a popular area of research especially in biological sciences because it makes an integral use of biochemistry, molecular biology and engineering sciences in order to achieve technological application of cultured tissues, cell and microbes. Plant tissue culture (PTC) refers to a technique of cultivation of plant cells and other parts on artificial nutrient medium in controlled environment under aseptic conditions. PTC requires various nutrients, pH, carbon source, gelling agent, temperature, photoperiod, humidity etc. and most importantly the judicious use of plant growth regulators. Various natural, adenine and phenyl urea derivatives are employed for the induction and proliferation of different types of explants. Several phenyl urea derivatives were evaluated and it was observed that thidiazuron (n-phenyl-N”-1,2,3- thidiazol-5-ulurea) was found to be the most active among the plant growth regulators. Thidiazuron (TDZ) was initially developed as a cotton defoliant and showed high cytokinin like activity. In some examples, its activity was 100 times more than BA in tobacco callus assay and produces more number of shoots in cultures than Zeatin and 2iP. TDZ also showed major breakthrough in tissue culture of various recalcitrant legumes and woody species. For the last two decades, number of laboratories has been working on TDZ with different aspect and number of publications has come out. To the best of our knowledge, there is no comprehensive edited volume on this particular topic. Hence th,e edited volume is a deed to consolidate the scattered information on role of TDZ in plant tissue culture and genetic manipulations that would hopefully prove informative to various researches. Thidiazuron: From Urea Derivative to Plant Growth Regulator compiles various aspects of TDZ in Plant Tissue Culture with profitable implications. The book will provides basic material for academicians and researchers who want to initiate work in this fascinating area of research. The book will contain 26 chapters compiled by International dignitaries and thus giving a holistic view to the edited volume.


Advances in Plant Tissue Culture

Advances in Plant Tissue Culture

Author: Avinash Chandra Rai

Publisher: Academic Press

Published: 2022-05-28

Total Pages: 428

ISBN-13: 0323998194

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Advances in Plant Tissue Culture: Current Developments and Future Trends provides a complete and up-to-date text on all basic and applied aspects of plant tissue cultures and their latest application implications. It will be beneficial for students and early-career researchers of plant sciences and plant/agricultural biotechnology. Plant tissue culture has emerged as a sustainable way to meet the requirements of fresh produces, horticultural crops, medicinal or ornamental plants. Nowadays, plant tissue culture is an emerging filed applied in various aspects, including sustainable agriculture, plant breeding, horticulture and forestry. This book covers the latest technology, broadly applied for crop improvement, clonal propagation, Somatic hybridization Embryo rescue, Germplasm conservation, genetic conservation, or for the preservation of endangered species. However, these technologies also play a vital role in breaking seed dormancy over conventional methods of conservation. - Focuses on plant tissue culture as an emerging field applied in various aspects, including sustainable agriculture, plant breeding, horticulture and forestry - Includes current studies and innovations in biotechnology - Covers commercialization and current perspectives in the field of plant tissue culture techniques


Plant Tissue Culture: Propagation, Conservation and Crop Improvement

Plant Tissue Culture: Propagation, Conservation and Crop Improvement

Author: Mohammad Anis

Publisher: Springer

Published: 2016-10-08

Total Pages: 616

ISBN-13: 9811019177

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This book presents basic concepts, methodologies and applications of biotechnology for the conservation and propagation of aromatic, medicinal and other economic plants. It caters to the needs and challenges of researchers in plant biology, biotechnology, the medical sciences, pharmaceutical biotechnology and pharmacology areas by providing an accessible and cost-effective practical approach to micro-propagation and conservation strategies for plant species. It also includes illustrations describing a complete documentation of the results and research into particular plant species conducted by the authors over the past 5 years. Plant Biotechnology has been a subject of academic interest for a considerable time. In recent years, it has also become a useful tool in agriculture and medicine, as well as a popular area of biological research. Current economic growth is globally projected in a highly positive manner, but the challenges many countries face with regard to food, feed, malnutrition, infectious diseases, the newly identified life-style diseases, and energy shortages, all of which are worsened by an ever-deteriorating environment, continue to pull the growth digits back. The common thread that connects all of the above challenges is biotechnology, which could provide many answers. Molecular biology and biotechnology have now become an integral part of tissue culture research. The tremendous impact generated by genetic engineering and consequently of transgenics now allows us to manipulate plant genomes at will. There has indeed been a rapid development in this area with major successes in both developed and developing countries. The book introduces several new and exciting areas to researchers who are unfamiliar with plant biotechnology and also serves as a review of ongoing research and future directions for scholars. The book highlights numerous methods for in vitro propagation and utilization of techniques in raising transgenics to help readers reproduce the experiments discussed.


Biostimulants in Plant Science

Biostimulants in Plant Science

Author: Seyed Mahyar Mirmajlessi

Publisher: BoD – Books on Demand

Published: 2020-07-08

Total Pages: 162

ISBN-13: 1838801618

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Natural-based substances, ‘plant biostimulants’, have been considered as environmentally friendly alternatives to agrichemicals. Biostimulants may comprise microbial inoculants, humic acids, fulvic acids, seaweed extracts, etc. These biostimulants have biopesticide and biostimulant utilities. Elucidations on direct or microbially mediated functions of biostimulants are presented in this book to illustrate fundamental principles and recent applications underlying this technology. This book has encompassed a cross-section of topics on different concepts to describe effective strategies by using these substances and/or beneficial microorganisms within sustainable agroecosystems. I sincerely hope that the information provided adequately reflects the objectives of this compilation. “One of the first conditions of happiness is that the link between man and nature shall not be broken.” Leo Tolstoy


Modern Applications of Plant Biotechnology in Pharmaceutical Sciences

Modern Applications of Plant Biotechnology in Pharmaceutical Sciences

Author: Saurabh Bhatia

Publisher: Academic Press

Published: 2015-07-22

Total Pages: 452

ISBN-13: 0128024984

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Modern Applications of Plant Biotechnology in Pharmaceutical Sciences explores advanced techniques in plant biotechnology, their applications to pharmaceutical sciences, and how these methods can lead to more effective, safe, and affordable drugs. The book covers modern approaches in a practical, step-by-step manner, and includes illustrations, examples, and case studies to enhance understanding. Key topics include plant-made pharmaceuticals, classical and non-classical techniques for secondary metabolite production in plant cell culture and their relevance to pharmaceutical science, edible vaccines, novel delivery systems for plant-based products, international industry regulatory guidelines, and more. Readers will find the book to be a comprehensive and valuable resource for the study of modern plant biotechnology approaches and their pharmaceutical applications. - Builds upon the basic concepts of cell and plant tissue culture and recombinant DNA technology to better illustrate the modern and potential applications of plant biotechnology to the pharmaceutical sciences - Provides detailed yet practical coverage of complex techniques, such as micropropogation, gene transfer, and biosynthesis - Examines critical issues of international importance and offers real-life examples and potential solutions


Plant Tissue Culture: An Introductory Text

Plant Tissue Culture: An Introductory Text

Author: Sant Saran Bhojwani

Publisher: Springer Science & Business Media

Published: 2013-03-20

Total Pages: 318

ISBN-13: 8132210263

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Plant tissue culture (PTC) is basic to all plant biotechnologies and is an exciting area of basic and applied sciences with considerable scope for further research. PTC is also the best approach to demonstrate the totipotency of plant cells, and to exploit it for numerous practical applications. It offers technologies for crop improvement (Haploid and Triploid production, In Vitro Fertilization, Hybrid Embryo Rescue, Variant Selection), clonal propagation (Micropropagation), virus elimination (Shoot Tip Culture), germplasm conservation, production of industrial phytochemicals, and regeneration of plants from genetically manipulated cells by recombinant DNA technology (Genetic Engineering) or cell fusion (Somatic Hybridization and Cybridization). Considerable work is being done to understand the physiology and genetics of in vitro embryogenesis and organogenesis using model systems, especially Arabidopsis and carrot, which is likely to enhance the efficiency of in vitro regeneration protocols. All these aspects are covered extensively in the present book. Since the first book on Plant Tissue Culture by Prof. P.R. White in 1943, several volumes describing different aspects of PTC have been published. Most of these are compilation of invited articles by different experts or proceedings of conferences. More recently, a number of books describing the Methods and Protocols for one or more techniques of PTC have been published which should serve as useful laboratory manuals. The impetus for writing this book was to make available a complete and up-to-date text covering all basic and applied aspects of PTC for the students and early-career researchers of plant sciences and plant / agricultural biotechnology. The book comprises of nineteen chapters profusely illustrated with self-explanatory illustrations. Most of the chapters include well-tested protocols and relevant media compositions that should be helpful in conducting laboratory experiments. For those interested in further details, Suggested Further Reading is given at the end of each chapter, and a Subject and Plant Index is provided at the end of the book.


Synthetic Seeds

Synthetic Seeds

Author: Mohammad Faisal

Publisher: Springer Nature

Published: 2019-11-23

Total Pages: 487

ISBN-13: 3030246310

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This book introduces the reader to synthetic or artificial seeds, which refer to alginate encapsulated somatic embryos, vegetative buds or any other micropropagules that can be used as seeds and converted into plantlets after propagating under in vitro or in vivo conditions. Moreover, synthetic seeds retain their potential for regeneration even after low-temperature storage. The production of synthetic or artificial seeds using micropropagules opens up new vistas in agricultural biotechnology. Encapsulated propagules could be used for in vitro regeneration and mass multiplication at reasonable cost. In addition, these propagules may be used for germplasm preservation of elite plant species and the exchange of plant materials between national and international laboratories. This book offers state-of-the-art findings on methods, applications and prospects of synthetic or artificial seeds.