Mammary Stem Cells
Mammary Stem Cells

Author: Maria del Mar Vivanco

Publisher: Humana

Published: 2015-06-04

Total Pages: 0

ISBN-13: 9781493925186

DOWNLOAD EBOOK

The identification of normal and breast cancer stem cells has offered a new vision of this heterogeneous disease and new hopes for its prognosis and treatment. This volume provides an overview of recent developments in mammary stem cell research and discusses the many varieties of approaches used by researchers to investigate the properties and functions of mammary stem cells. The beginning chapters provide readers with an introduction to mammary stem cells, and the processes used to characterize stem cells and isolate them via fluorescent activated cell sorting. The next few chapters discuss DNA and mRNA sequencing, proteomic techniques to help profile cells, lentiviral cell transduction for gene expression, and in vivo lineage tracing. The final few chapters are dedicated to following stem cells from their initial niche to the new microenvironment at their metastasis site, and to studying these cells using physical and mathematical approaches. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Authoritative and cutting-edge, Mammary Stem Cells: Methods and Protocols aims to help members of the scientific community explore the behavior of stem cells and how to work with them in order to guide the design of new and complimentary strategies to be applied in the clinic with the ultimate end goal of fighting breast cancer.

Mammary Stem Cells
Mammary Stem Cells

Author: Maria dM. Vivanco

Publisher: Humana

Published: 2022-02-18

Total Pages: 354

ISBN-13: 9781071621929

DOWNLOAD EBOOK

This second edition provides an overview of recent developments and approaches used by researchers to investigate the properties and functions of mammary epithelial and stem cells, which will contribute to understand the heterogeneity of the mammary gland and of breast cancer. Chapters detail processes used to characterize stem cells, single cell RNA sequencing, computational methods, sophisticated imaging techniques, and a variety of model systems, among others. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mammary Stem Cells: Methods and Protocols, Second Edition aims to make available protocols used to navigate the intricate behavior of mammary stem cells and to gain further knowledge to take us closer to the design of innovative strategies to prevent and treat breast cancer.

Stem Cells
Stem Cells

Author: C. Potten

Publisher: Elsevier

Published: 1996-10-09

Total Pages: 497

ISBN-13: 0080541763

DOWNLOAD EBOOK

Stem cells are relatively undifferentiated cells which are the permanent lineage ancestor cells of tissues. Newly developed molecular biological techniques and probes have made possible dramatic advances in our ability to study the lineage development of stem cells. A major impetus to develop these techniques has been to identify specific stem cells for gene therapy purposes. The role that stem cells play in the development of cancer is also an important area. This book provides up-to-date reviews on a wide variety of stem cell systems by world experts. Chapters range from descriptions of the current knowledge of the biology of stem cells, to current molecular biological approaches and clinical implications. Oncologists and cell biologists will find this book of particular interest. It will also be usefule to radiobiologist, biotechnologists, and gene therapists. Provides reviews of stem cell systems by world experts Covers stem cell biology in plants, invertebrates, and mammals Presents clinical implications of stem cell differentiation

Stem Cells in Veterinary Science
Stem Cells in Veterinary Science

Author: Ratan Kumar Choudhary

Publisher: Springer Nature

Published: 2022-02-05

Total Pages: 375

ISBN-13: 9811634645

DOWNLOAD EBOOK

This book explores the potential applications of animal stem cells in veterinary medicine. It begins with an overview of stem cells and their application in treating various animal diseases, including mastitis. In turn, the book discusses the challenges of using stem cells in regenerative medicine and emphasizes the importance of understanding the action of stem cells and preclinical evidence for ensuring safety and therapeutic efficacy. It also presents methods for the identification, characterization, and quantification of stem cells. Further, it discusses the therapeutic applications of different stem cells, including milk-derived, testicular, and mesenchymal stem cells in veterinary medicine. Lastly, it discusses strategies for and therapeutic applications of genome editing by CRISPER/Cas9 in mammary stem cells. As such, the book offers a valuable resource for students and scientists working in the veterinary sciences and veterinarians.

Of Microenvironments and Mammary Stem Cells
Of Microenvironments and Mammary Stem Cells

Author:

Publisher:

Published: 2007

Total Pages: 137

ISBN-13:

DOWNLOAD EBOOK

In most adult tissues there reside pools of stem and progenitor cells inside specialized microenvironments referred to as niches. The niche protects the stem cells from inappropriate expansion and directs their critical functions. Thus guided, stem cells are able to maintain tissue homeostasis throughout the ebb and flow of metabolic and physical demands encountered over a lifetime. Indeed, a pool of stem cells maintains mammary gland structure throughout development, and responds to the physiological demands associated with pregnancy. This review discusses how stem cells were identified in both human and mouse mammary glands; each requiring different techniques that were determined by differing biological needs and ethical constraints. These studies together create a robust portrait of mammary gland biology and identify the location of the stem cell niche, elucidate a developmental hierarchy, and suggest how the niche might be manipulated for therapeutic benefit.

The Breast
The Breast

Author: Helmuth Vorherr

Publisher: Elsevier

Published: 2012-12-02

Total Pages: 295

ISBN-13: 0323157262

DOWNLOAD EBOOK

The Breast: Morphology, Physiology, and Lactation covers the mammary morphology and function related to endocrine physiology, as well as the pathophysiologic disorders, such as galactorrhea. Knowledge of the many hormones influencing the structure and function of the breast enables one to relate cyclic endocrine ovarian changes to symptoms of premenstrual mammary tension, parenchymal alterations, and breast neoplasia. This book is divided into nine chapters and begins with a description of the female breast development. This topic is followed by a discussion on the morphological aspects of the mature female breast, including the anatomy of the mammary gland, breast changes during pregnancy, and milk synthesis. The next chapters focus on the process of lactation and the hormonal mechanisms involved in lactation maintenance. Other chapters consider the techniques, principles, and advantages of breast feeding, as well as the concept of lactation suppression. The final chapters examine the syndromes, potential causes, and management galactorrhea, which is associated with increased pituitary prolactin secretion and deficient gonadotropin production. This book is of value to medical students, physiologists, endocrinologists, pharmacologists, basic and clinical investigators, physicians, and clinicians.

Characterization of Human Mammary Epithelial Stem Cells
Characterization of Human Mammary Epithelial Stem Cells

Author:

Publisher:

Published: 2009

Total Pages: 18

ISBN-13:

DOWNLOAD EBOOK

The mammary epithelium of normal adult female mice contains stem cells with extensive in vivo regenerative and self-renewal potential. Indirect evidence has suggested that analogous cells exist in the mammary glands of adult women, and are candidate targets for the first transforming mutations that lead to the evolution of breast cancer stem cells. The objective of this grant was to determine whether these hypothesized normal human mammary stem cells might be detected and quantified by a robust and specific assay that could be used to enable the purification and phenotypic properties of these cells, and to derive information about their frequency and how they are regulated. During the 3 years of this grant, I established conditions that allow human mammary gland structures to be reproducibly generated in subrenal xenografts in highly immune deficient mice, starting with small innocula of dissociated human mammary cells. The regenerated glands are similar in morphology and cellular organization to normal human mammary glands, bounded by a basement membrane with an outer layer of myo-epithelial cells and an inner layer of polarized luminal cells that can be induced to produce milk. I also established that the presence of regenerated structures can be determined by detecting the in vitro clonogenic progenitors they contain and this endpoint can serve as an objective indicator of the presence of a primitive stem-like cell in the initial cells transplanted. This retrospective functional assay allows limiting dilution analysis of positive xenograft yields to derive mammary stem cell frequencies in differently manipulated populations. Using this approach I found the frequency of stem cells in normal human mammary tissue to be ~1 per 5000 cells and their phenotype to be CD49f+ EpCAM-/low CD31- CD45-

Culture of Human Stem Cells
Culture of Human Stem Cells

Author: R. Ian Freshney

Publisher: John Wiley & Sons

Published: 2007-07-16

Total Pages: 360

ISBN-13: 9780470167519

DOWNLOAD EBOOK

This book collects the most effective and cutting-edge methods and protocols for deriving and culturing human embryonic and adult stem cells—in one handy resource. This groundbreaking book follows the tradition of previous books in the Culture of Specialized Cells Series—each methods and protocols chapter is laid out exactly like the next, with stepwise protocols, preceded by specific requirements for that protocol, and a concise discussion of methods illustrated by data. The editors describe a limited number of representative techniques across a wide spectrum of stem cells from embryonic, newborn, and adult tissue, yielding an all-encompassing and versatile guide to the field of stem cell biology and culture. The book includes a comprehensive list of suppliers for all equipment used in the protocols presented, with websites available in an appendix. Additionally, there is a chapter on quality control, and other chapters covering legal and ethical issues, cryopreservation, and feeder layer culture. This text is a one-stop resource for all researchers, clinical scientists, teachers, and students involved in this crucial area of study.

Biology of the Mammary Gland
Biology of the Mammary Gland

Author: John A. Mol

Publisher: Springer Science & Business Media

Published: 2006-04-11

Total Pages: 309

ISBN-13: 0306468328

DOWNLOAD EBOOK

Proceedings of the European Cooperation in the Field of Scientific and Technical Research (COST 825) Symposium on Mammary Gland Biology, held September 16-18, 1999, in Tours, France. It is difficult to overstate the evolutionary and functional significance of mammary tissue in biology. Substantial progress has been made by researchers in various disciplines, particularly over the last fifteen years, towards realizing the potential of this tissue to yield powerful experimental models for morphogenesis and tissue development; for cellular differentiation; for the biosynthesis and secretion of proteins, lipids, small molecules and inorganic salts; and for the coordination and regulation of these processes. More recently, the possibility of exploiting the secretory epithelial cells of mammary tissue as `cell factories' has become a reality and the recombinant production by lactating animals of an increasing number of proteins, valuable both in the pharmaceutical and `nutraceutical' fields, is in progress or under development. Also in this sphere of agricultural production, genetic as well as nutritional technologies are under investigation and exploitation to optimize milk composition for various end-uses - for instance in food process and manufacture. The possibilities of deriving health benefit from the bioactive properties of some of the minor constituents of milk are emerging to counter the highly-publicized negative health impact of excessive consumption of saturated animal fats. In human nutrition and medicine, the mammary gland is both a source of nutrition to the neonate and a potential health threat to the adult female - breast cancer remains the major single cause of female mortality in most developed countries. This volume provides a unique glimpse into our understanding, at the cutting edge of a variety of disciplines, of this versatile and extraordinary tissue, at the birth of the twenty-first century.

Breast Cancer
Breast Cancer

Author: Phuc Van Pham

Publisher: BoD – Books on Demand

Published: 2017-04-05

Total Pages: 570

ISBN-13: 9535129996

DOWNLOAD EBOOK

Breast Cancer - From Biology to Medicine thoroughly examines breast cancer from basic definitions, to cellular and molecular biology, to diagnosis and treatment. This book also has some additional focus on preclinical and clinical results in diagnosis and treatment of breast cancer. The book begins with introduction on epidemiology and pathophysiology of breast cancer in Section 1. In Section 2, the subsequent chapters introduce molecular and cellular biology of breast cancer with some particular signaling pathways, the gene expression, as well as the gene methylation and genomic imprinting, especially the existence of breast cancer stem cells. In Section 3, some new diagnostic methods and updated therapies from surgery, chemotherapy, hormone therapy, immunotherapy, radiotherapy, and some complementary therapies are discussed. This book provides a succinct yet comprehensive overview of breast cancer for advanced students, graduate students, and researchers as well as those working with breast cancer in a clinical setting.