Handbook of Biomedical Nonlinear Optical Microscopy

Handbook of Biomedical Nonlinear Optical Microscopy

Author: Barry R. Masters

Publisher: Oxford University Press

Published: 2008-05-19

Total Pages: 895

ISBN-13: 0198036825

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The Handbook of Biomedical Nonlinear Optical Microscopy provides comprehensive treatment of the theories, techniques, and biomedical applications of nonlinear optics and microscopy for cell biologists, life scientists, biomedical engineers, and clinicians. The chapters are separated into basic and advanced sections, and provide both textual and graphical illustrations of all key concepts. The more basic sections are aimed at life scientists without advanced training in physics and mathematics, and tutorials are provided for the more challenging sections. The first part of the Handbook introduces the historical context of nonlinear microscopy. The second part presents the nonlinear optical theory of two- and multiphoton excited fluorescence (TPE, MPE) spectroscopy, second and third harmonic generation (SHG, THG) spectroscopy, and coherent anti-Stokes Raman spectroscopy (CARS). The third part introduces modern microscopic and spectroscopic instrumentation and techniques that are based on nonlinear optics. The fourth part provides key applications of nonlinear microscopy to the biomedical area: neurobiology, immunology, tumor biology, developmental biology, dermatology, and cellular metabolism. There are also chapters on nonlinear molecular probes, cellular damage, and nanoprocessing.


Linear and Nonlinear Optics

Linear and Nonlinear Optics

Author: Kitsakorn Locharoenrat

Publisher: CRC Press

Published: 2021-01-31

Total Pages: 345

ISBN-13: 1000091848

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In recent years, optical properties of the unique atomic and molecular structures of materials have drawn great scientific interest. Linear optical properties of materials such as metals, metal oxides, magnetic oxides, and organic materials are based on energy transfer and find applications in wastewater treatment, forensic science, biomedical science, photovoltaics, nuclear technology, and LED displays. Nonlinear optical properties of materials are based on the nonlinear medium and find more advanced applications in frequency mixing generations and optical parametric oscillations. This book presents the underlying principles, implementation, and applications of the linear and nonlinear optical properties of materials and has been divided into two parts emphasizing these properties. The first part of the book, Linear Optics, discusses bimetallic nanoparticles in dielectric media and their integration to dye molecules to detect trace amounts of heavy metals at the nanometer level, as well as to enhance luminescence and image contrasts in forensic inspection and biomedical diagnosis. It shows how the integration of bimetallic nanoparticles into a ZnO matrix promotes broadening of the absorption spectrum from the ultraviolet to the visible wavelength. It explains the role of surface adsorption and photocatalytic degradation in dye-removal kinetics by Fe3O4 magnetic nanoparticles under pulsed white light. It also discusses the double-layer shielding tank design to safely store radioactive waste and photon propagation through the multilayer structures of a human tissue model. The second part of the book, Nonlinear Optics, presents general concepts such as electromagnetic theory, nonlinear medium, and wave propagation, as well as more advanced concepts such as second harmonic generation, phase matching, optical parametric interactions, different frequency generation, sum frequency generation, tunable laser, and optical resonant oscillator.


Raman Microscopy

Raman Microscopy

Author: George Turrell

Publisher: Academic Press

Published: 1996-06-24

Total Pages: 493

ISBN-13: 0080540252

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One of the first books devoted entirely to the subject of Raman microscopy, Raman Microscopy addresses issues of great interest to engineers working in Raman-microscope development and researchers concerned with areas ofapplication for this science. The book is written by several world recognized experts, who summarize the Raman effect before discussing the hardware and software involved in todays instruments. This format provides an excellent introduction to this up-and-coming discipline. All important applications, including those in materials science and earth science are covered in depth. - Includes extensive description of the instrumentation, the Raman microspectrograph, the treatment of data, and micro-Raman imaging - Examines the use of Raman microscopy in diverse applications, including some of the hyphenated methods - Summarizes the Raman effect - Discusses new uses for this technology


Second Harmonic Generation Imaging

Second Harmonic Generation Imaging

Author: Francesco S. Pavone

Publisher: Taylor & Francis

Published: 2016-04-19

Total Pages: 465

ISBN-13: 1439849153

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Second-harmonic generation (SHG) microscopy has shown great promise for imaging live cells and tissues, with applications in basic science, medical research, and tissue engineering. Second Harmonic Generation Imaging offers a complete guide to this optical modality, from basic principles, instrumentation, methods, and image analysis to biomedical a


Dispersion, Complex Analysis and Optical Spectroscopy

Dispersion, Complex Analysis and Optical Spectroscopy

Author: Kai-Erik Peiponen

Publisher: Springer Science & Business Media

Published: 1998-11-30

Total Pages: 152

ISBN-13: 9783540645221

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This book is devoted to dispersion theory in linear and nonlinear optics. Dispersion relations and methods of analysis in optical spectroscopy are derived with the aid of complex analysis. The book introduces the mathematical basis and derivations of various dispersion relations that are used in optical spectroscopy. In addition, it presents the dispersion theory of the nonlinear optical processes which are essential in modern optical spectroscopy. The book includes new methods such as the maximum entropy model for wavelength-dependent spectra analysis.


Microscopy Techniques

Microscopy Techniques

Author: Jens Rietdorf

Publisher: Springer Science & Business Media

Published: 2005-06-23

Total Pages: 344

ISBN-13: 9783540236986

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With contributions by numerous experts


Biochemical Applications of Nonlinear Optical Spectroscopy

Biochemical Applications of Nonlinear Optical Spectroscopy

Author: Vladislav V. Yakovlev

Publisher: CRC Press

Published: 2018-10-08

Total Pages: 296

ISBN-13: 1420068601

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For a host of reasons, nonlinear optical spectroscopy is a valuable tool for biochemical applications where minimally invasive diagnostics is desired. Biochemical Applications of Nonlinear Optical Spectroscopy presents the latest technological advances and offers a perspective on future directions in this important field. Written by an international panel of experts, this volume begins with a comparison of nonlinear optical spectroscopy and x-ray crystallography. The text examines the use of multiphoton fluorescence to study chemical phenomena in the skin, the use of nonlinear optics to enhance traditional optical spectroscopy, and the multimodal approach, which incorporates several spectroscopic techniques in one instrument. Later chapters explore Raman microscopy, third-harmonic generation microscopy, and non-linear Raman microspectroscopy. The text explores the promise of beam shaping and the use of broadband laser pulse generated through continuum generation and an optical pulse shaper. Lastly, the book discusses the effects of spatial beam shaping on the generated nonlinear Raman signals in a tightly focused geometry and provides insight into the extension of nonlinear optical spectroscopy to the nanoscale through the use of plasmonic tip-enhanced arrangement. With novel experimental approaches to this technology expanding day-by-day, the book’s balanced coverage from a wide range of international contributors not only elucidates important achievements, but also outlines future directions in this dynamic and promising field.