The Proteomics Protocols Handbook
The Proteomics Protocols Handbook

Author: John M. Walker

Publisher: Springer Science & Business Media

Published: 2007-10-09

Total Pages: 969

ISBN-13: 1592598900

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Hands-on researchers describe in step-by-step detail 73 proven laboratory methods and bioinformatics tools essential for analysis of the proteome. These cutting-edge techniques address such important tasks as sample preparation, 2D-PAGE, gel staining, mass spectrometry, and post-translational modification. There are also readily reproducible methods for protein expression profiling, identifying protein-protein interactions, and protein chip technology, as well as a range of newly developed methodologies for determining the structure and function of a protein. The bioinformatics tools include those for analyzing 2D-GEL patterns, protein modeling, and protein identification. All laboratory-based protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Handbook Of Synthetic Methodologies And Protocols Of Nanomaterials (In 4 Volumes)
Handbook Of Synthetic Methodologies And Protocols Of Nanomaterials (In 4 Volumes)

Author:

Publisher: World Scientific

Published: 2019-08-13

Total Pages: 2370

ISBN-13: 9813277882

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This comprehensive book set includes four volumes, covering the methods and protocols for the synthesis, fabrication, and characterization of nanomaterials. The first two books introduce the solution phase and gas synthesis approaches for nanomaterials, providing a number of most widely used protocols for each nanomaterial. An exhaustive list of nanomaterials are included, which are arranged according to the atomic number of the main element in the compound for easy search. For each material, the protocols are categorized according to the morphology of the nanostructure. A detailed reference is included in each protocol to point the readers to the source of the protocol. The third book describes many unconventional methods for the fabrication of nanostructures, including lithography and printing, self-assembly, chemical transformation, templated synthesis, electrospinning, laser induced synthesis, flame and plasma synthesis, and atomic layer deposition processes. The fourth book covers the typical methods for structural characterization of nanomaterials, including electron diffraction, electron microscopy, atomic force microscopy, scanning tunneling microscopy, X-ray diffraction, in-situ and operando X-ray techniques, X-ray absorption fine structure spectroscopy, static and dynamic light scattering, vibrational characterization methods, and NMR spectroscopy. In addition to the introduction of the basic operational principles of these tools, the book focuses explicitly on how they can be applied for analyzing nanomaterials. The handbook is a complete reference that can provide readers easily accessible information on how to synthesize and characterize nanomaterials desired for their target applications.

Pre-Field Screening Protocols for Heat-Tolerant Mutants in Rice
Pre-Field Screening Protocols for Heat-Tolerant Mutants in Rice

Author: Fatma Sarsu

Publisher: Springer

Published: 2018-08-09

Total Pages: 51

ISBN-13: 3319773380

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This open access book presents simple, robust pre-field screening protocols that allow plant breeders to screen for enhanced tolerance to heat stress in rice. Two critical heat-sensitive stages in the lifecycle of the rice crop are targeted – the seedling and flowering stages – with screening based on simple phenotypic responses. The protocols are based on the use of a hydroponics system and/or pot experiments in a glasshouse in combination with a controlled growth chamber where the heat stress treatment is applied. The protocols are designed to be effective, simple, reproducible and user-friendly. The protocols will enable plant breeders to effectively reduce the number of plants from a few thousands to less than 100 candidate individual mutants or lines in a greenhouse/growth chamber, which can then be used for further testing and validation in the field conditions. The methods can also be used to classify rice genotypes according to their heat tolerance characteristics. Thus, different types of heat stress tolerance mechanisms can be identified, presenting opportunities for pyramiding different (mutant) sources of heat stress tolerance.

Laboratory Protocols in Applied Life Sciences
Laboratory Protocols in Applied Life Sciences

Author: Prakash Singh Bisen

Publisher: CRC Press

Published: 2014-02-26

Total Pages: 1772

ISBN-13: 1466553154

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As applied life science progresses, becoming fully integrated into the biological, chemical, and engineering sciences, there is a growing need for expanding life sciences research techniques. Anticipating the demands of various life science disciplines, Laboratory Protocols in Applied Life Sciences explores this development. This book covers a wide spectrum of areas in the interdisciplinary fields of life sciences, pharmacy, medical and paramedical sciences, and biotechnology. It examines the principles, concepts, and every aspect of applicable techniques in these areas. Covering elementary concepts to advanced research techniques, the text analyzes data through experimentation and explains the theory behind each exercise. It presents each experiment with an introduction to the topic, concise objectives, and a list of necessary materials and reagents, and introduces step-by-step, readily feasible laboratory protocols. Focusing on the chemical characteristics of enzymes, metabolic processes, product and raw materials, and on the basic mechanisms and analytical techniques involved in life science technological transformations, this text provides information on the biological characteristics of living cells of different origin and the development of new life forms by genetic engineering techniques. It also examines product development using biological systems, including pharmaceutical, food, and beverage industries. Laboratory Protocols in Applied Life Sciences presents a nonmathematical account of the underlying principles of a variety of experimental techniques in disciplines, including: Biotechnology Analytical biochemistry Clinical biochemistry Biophysics Molecular biology Genetic engineering Bioprocess technology Industrial processes Animal Plant Microbial biology Computational biology Biosensors Each chapter is self-contained and written in a style that helps students progress from basic to advanced techniques, and eventually design and execute their own experiments in a given field of biology.

Proteoglycan Protocols
Proteoglycan Protocols

Author: Renato V. Iozzo

Publisher: Springer Science & Business Media

Published: 2008-02-02

Total Pages: 547

ISBN-13: 1592592090

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Proteoglycans are some of the most elaborate macromolecules of mammalian and lower organisms. The covalent attachment of at least five types of glycosami- glycan side chains to more than forty individual protein cores makes these molecules quite complex and endows them with a multitude of biological functions. Proteoglycan Protocols offers a comprehensive and up-to-date collection of prepa- tive and analytical methods for the in-depth analysis of proteoglycans. Featuring st- by-step detailed protocols, this book will enable both novice and experienced researchers to isolate intact proteoglycans from tissues and cultured cells, to establish the composition of their carbohydrate moieties, to generate strategies for prokaryotic and eukaryotic expression, to utilize methods for the suppression of specific proteoglycan gene expression and for the detection of mutant cells and degradation products, and to study specific interactions between proteoglycans and extracellular matrix proteins as well as growth factors and their receptors. The readers will find concise, yet comprehensive techniques carefully drafted by leading experts in the field. Each chapter commences with a general Introduction, followed by a detailed Materials section, and an easy-to-follow Methods section. An asset of each chapter is the extensive notation that includes troubleshooting tips and practical considerations that are often lacking in formal methodology papers. The reader will find this section most valuable because it is clearly provided by experienced scientists who have first-hand knowledge of the techniques they outline. In addition, most of the chapters are well illustrated with examples of typical data generated with each method.

Water Oxidation Catalysts
Water Oxidation Catalysts

Author:

Publisher: Academic Press

Published: 2019-06-22

Total Pages: 382

ISBN-13: 0128160837

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Water Oxidation Catalysts, Volume 74, the latest release in the Advances in Inorganic Chemistry series, presents timely and informative summaries on current progress in a variety of subject areas. This acclaimed serial features reviews written by experts in the field, serving as an indispensable reference to advanced researchers. Users will find this to be a comprehensive overview of recent findings and trends from the last decade that covers various kinds of inorganic topics, ranging from theoretical oriented supramolecular chemistry, to the quest for accurate calculations of spin states in transition metals. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Advances in Inorganic Chemistry series - Includes the latest information on water oxidation catalysts

Micro Total Analysis Systems 2000
Micro Total Analysis Systems 2000

Author: Albert van den Berg

Publisher: Springer Science & Business Media

Published: 2013-11-11

Total Pages: 639

ISBN-13: 9401722641

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This volume contains the proceedings of the fourth international symposium on Micro Total Analysis Systems (muTAS 2000). Cutting-edge research of all invited and contributed papers presented by the world’s leading muTAS groups provides the state of the art of this electrifying, multidisciplinary field.