Guide to Research Techniques in Neuroscience
Guide to Research Techniques in Neuroscience

Author: Matt Carter

Publisher: Academic Press

Published: 2022-03-26

Total Pages: 416

ISBN-13: 0323915612

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Modern neuroscience research is inherently multidisciplinary, with a wide variety of cutting edge new techniques to explore multiple levels of investigation. This Third Edition of Guide to Research Techniques in Neuroscience provides a comprehensive overview of classical and cutting edge methods including their utility, limitations, and how data are presented in the literature. This book can be used as an introduction to neuroscience techniques for anyone new to the field or as a reference for any neuroscientist while reading papers or attending talks. - Nearly 200 updated full-color illustrations to clearly convey the theory and practice of neuroscience methods - Expands on techniques from previous editions and covers many new techniques including in vivo calcium imaging, fiber photometry, RNA-Seq, brain spheroids, CRISPR-Cas9 genome editing, and more - Clear, straightforward explanations of each technique for anyone new to the field - A broad scope of methods, from noninvasive brain imaging in human subjects, to electrophysiology in animal models, to recombinant DNA technology in test tubes, to transfection of neurons in cell culture - Detailed recommendations on where to find protocols and other resources for specific techniques - "Walk-through" boxes that guide readers through experiments step-by-step

In Vivo Optical Imaging of Brain Function
In Vivo Optical Imaging of Brain Function

Author: Ron D. Frostig

Publisher: CRC Press

Published: 2009-05-06

Total Pages: 494

ISBN-13: 142007685X

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These are exciting times for the field of optical imaging of brain function. Rapid developments in theory and technology continue to considerably advance understanding of brain function. Reflecting changes in the field during the past five years, the second edition of In Vivo Optical Imaging of Brain Function describes state-of-the-art techniques a

High Resolution Imaging in Microscopy and Ophthalmology
High Resolution Imaging in Microscopy and Ophthalmology

Author: Josef F. Bille

Publisher: Springer

Published: 2019-08-13

Total Pages: 411

ISBN-13: 3030166384

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This open access book provides a comprehensive overview of the application of the newest laser and microscope/ophthalmoscope technology in the field of high resolution imaging in microscopy and ophthalmology. Starting by describing High-Resolution 3D Light Microscopy with STED and RESOLFT, the book goes on to cover retinal and anterior segment imaging and image-guided treatment and also discusses the development of adaptive optics in vision science and ophthalmology. Using an interdisciplinary approach, the reader will learn about the latest developments and most up to date technology in the field and how these translate to a medical setting. High Resolution Imaging in Microscopy and Ophthalmology – New Frontiers in Biomedical Optics has been written by leading experts in the field and offers insights on engineering, biology, and medicine, thus being a valuable addition for scientists, engineers, and clinicians with technical and medical interest who would like to understand the equipment, the applications and the medical/biological background. Lastly, this book is dedicated to the memory of Dr. Gerhard Zinser, co-founder of Heidelberg Engineering GmbH, a scientist, a husband, a brother, a colleague, and a friend.

Modern Techniques in Neuroscience Research
Modern Techniques in Neuroscience Research

Author: Uwe Windhorst

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 1336

ISBN-13: 3642585523

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An overview of the techniques used in modern neuroscience research with the emphasis on showing how different techniques can optimally be combined in the study of problems that arise at some levels of nervous system organization. This is essentially a working tool for the scientist in the laboratory and clinic, providing detailed step-by-step protocols with tips and recommendations. Most chapters and protocols are organized such that they can be used independently, while cross-references between the chapters, a glossary, a list of suppliers and appendices provide further help.

Brain Aging
Brain Aging

Author: David R. Riddle

Publisher: CRC Press

Published: 2007-04-19

Total Pages: 413

ISBN-13: 1420005529

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Recognition that aging is not the accumulation of disease, but rather comprises fundamental biological processes that are amenable to experimental study, is the basis for the recent growth of experimental biogerontology. As increasingly sophisticated studies provide greater understanding of what occurs in the aging brain and how these changes occur

Handbook of Optical Biomedical Diagnostics
Handbook of Optical Biomedical Diagnostics

Author: Valeriĭ Viktorovich Tuchin

Publisher:

Published: 2016

Total Pages: 688

ISBN-13: 9781628419122

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This text begins by describing the basic principles and diagnostic applications of optical techniques based on detecting and processing the scattering, fluorescence, FT IR, and Raman spectroscopic signals from various tissues, with an emphasis on blood, epithelial tissues, and human skin. The second half of the volume discusses specific imaging technologies, such as Doppler, laser speckle, optical coherence tomography (OCT), and fluorescence and photoacoustic imaging.

Neural Metabolism In Vivo
Neural Metabolism In Vivo

Author: In-Young Choi

Publisher: Springer Science & Business Media

Published: 2012-03-08

Total Pages: 1168

ISBN-13: 1461417880

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From the preface: “Neural Metabolism In Vivo aims to provide a comprehensive overview of neurobiology by presenting the basic principles of up-to-date and cutting-edge technology, as well as their application in assessing the functional, morphological and metabolic aspects of the brain. Investigation of neural activity of the living brain via neurovascular coupling using multimodal imaging techniques extended our understanding of fundamental neurophysiological mechanisms, regulation of cerebral blood flow in connection to neural activity and the interplay between neurons, astrocytes and blood vessels. Constant delivery of glucose and oxygen for energy metabolism is vital for brain function, and the physiological basis of neural activity can be assessed through measurements of cerebral blood flow and consumption of glucose and oxygen.... This book presents the complex physiological and neurochemical processes of neural metabolism and function in response to various physiological conditions and pharmacological stimulations. Neurochemical detection technologies and quantitative aspects of monitoring cerebral energy substrates and other metabolites in the living brain are described under the “Cerebral metabolism of antioxidants, osmolytes and others in vivo” section. Altogether, the advent of new in vivo tools has transformed neuroscience and neurobiology research, and demands interdisciplinary approaches as each technology could only approximate a very small fraction of the true complexity of the underlying biological processes. However, translational values of the emerging in vivo methods to the application of preclinical to clinical studies cannot be emphasized enough. Thus, it is our hope that advances in our understanding of biochemical, molecular, functional and physiological processes of the brain could eventually help people with neurological problems, which are still dominated by the unknowns.” -- In-Young Choi and Rolf Gruetter

Methods for Neural Ensemble Recordings
Methods for Neural Ensemble Recordings

Author: Miguel A. L. Nicolelis

Publisher: CRC Press

Published: 2007-12-03

Total Pages: 308

ISBN-13: 142000641X

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Extensively updated and expanded, this second edition of a bestseller distills the current state-of-the-science and provides the nuts and bolts foundation of the methods involved in this rapidly growing science. With contributions from pioneering researchers, it includes microwire array design for chronic neural recordings, new surgical techniques for chronic implantation, microelectrode microstimulation of brain tissue, multielectrode recordings in the somatosensory system and during learning, as well as recordings from the central gustatory-reward pathways. It explores the use of Brain-Machine Interface to restore neurological function and proposes conceptual and technical approaches to human neural ensemble recordings in the future.

Biology of Pericytes – Recent Advances
Biology of Pericytes – Recent Advances

Author: Alexander Birbrair

Publisher: Springer Nature

Published: 2021-04-08

Total Pages: 283

ISBN-13: 3030621294

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Pericytes were originally discovered and named more than hundred years ago as contractile cells around the blood vessel endothelial cells. Due to the lack of exclusive markers, pericytes are now defined by a combination of location, morphology and gene expression. Pericytes are attracting increasing attention as important regulators during development and during normal and disturbed organ function. In recent years, remarkable progress has been made in the identification and characterization of pericytes subpopulations and their amazing functions using state-of-art techniques. These advantages facilitated identification of molecular basis of interaction between these cells with several other more well studied cell types, and revealed key signals derived from pericytes involved in homeostasis, regeneration, and disease regulation. In the last ten years, several unexpected roles of pericytes have been discovered. It has been demonstrated that pericytes from different tissues differ in their properties as well as functions. Even more, pericytes are heterogeneous also within the same organ. This book is will describe the major contributions of pericytes to different organs biology in physiological and pathological conditions. The book will teach the readers about this so special cell type that 10 years ago was almost completely forgotten, and it was associated basically only with vascular stability. Recently, it become a very hot topic to work in. Several articles in Nature, Science and Cell have been and are being currently published about this cell type. These recent works are revealing how important those cells are for before unimaginable biological processes. Thus, this book will update us on what are the most novel functions attributed to these cells. Also, will introduce to the young generation all the history about these cells from when they were discovered in different organs till where we are now in this field. So it will be a great book for both cell biology students as well as researchers that will have an update on these cells biology in different organs.

Biomedical Optical Imaging
Biomedical Optical Imaging

Author: James G. Fujimoto

Publisher: Oxford University Press

Published: 2009-04-22

Total Pages: 435

ISBN-13: 0199722293

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Biomedical optical imaging is a rapidly emerging research area with widespread fundamental research and clinical applications. This book gives an overview of biomedical optical imaging with contributions from leading international research groups who have pioneered many of these techniques and applications. A unique research field spanning the microscopic to the macroscopic, biomedical optical imaging allows both structural and functional imaging. Techniques such as confocal and multiphoton microscopy provide cellular level resolution imaging in biological systems. The integration of this technology with exogenous chromophores can selectively enhance contrast for molecular targets as well as supply functional information on processes such as nerve transduction. Novel techniques integrate microscopy with state-of-the-art optics technology, and these include spectral imaging, two photon fluorescence correlation, nonlinear nanoscopy; optical coherence tomography techniques allow functional, dynamic, nanoscale, and cross-sectional visualization. Moving to the macroscopic scale, spectroscopic assessment and imaging methods such as fluorescence and light scattering can provide diagnostics of tissue pathology including neoplastic changes. Techniques using light diffusion and photon migration are a means to explore processes which occur deep inside biological tissues and organs. The integration of these techniques with exogenous probes enables molecular specific sensitivity.