In Vivo Optical Imaging of Brain Function, Second Edition

In Vivo Optical Imaging of Brain Function, Second Edition

Author: Ron D. Frostig

Publisher: CRC Press

Published: 2009-05-06

Total Pages: 0

ISBN-13: 9781420076844

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These are exciting times for the field of optical imaging of brain function. Rapid developments in theory and technology continue to considerably advance understanding of brain function. Reflecting changes in the field during the past five years, the second edition of In Vivo Optical Imaging of Brain Function describes state-of-the-art techniques and their applications for the growing field of functional imaging in the live brain using optical imaging techniques. New in the Second Edition: Voltage-sensitive dyes imaging in awake behaving animals Imaging based on genetically encoded probes Imaging of mitochondrial auto-fluorescence as a tool for cortical mapping Using pH-sensitive dyes for functional mapping Modulated imaging Calcium imaging of neuronal activity using 2-photon microscopy Fourier approach to optical imaging Fully updated chapters from the first edition Leading Authorities Explore the Latest Techniques Updated to reflect continuous development in this emerging research area, this new edition, as with the original, reaches across disciplines to review a variety of non-invasive optical techniques used to study activity in the living brain. Leading authorities from such diverse areas as biophysics, neuroscience, and cognitive science present a host of perspectives that range from a single neuron to large assemblies of millions of neurons, captured at various temporal and spatial resolutions. Introducing techniques that were not available just a few years ago, the authors describe the theory, setup, analytical methods, and examples that highlight the advantages of each particular method.


Imaging in Neuroscience

Imaging in Neuroscience

Author: Fritjof Helmchen

Publisher:

Published: 2011

Total Pages: 0

ISBN-13: 9780879699376

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As imaging technologies have revolutionized research in many areas of biology and medicine, neuroscientists have often pioneered the use of these new visualization techniques. This volume is an essential guide to discovering and implementing these techniques in the neuroscience lab.


Cellular Imaging Techniques for Neuroscience and Beyond

Cellular Imaging Techniques for Neuroscience and Beyond

Author: Floris G. Wouterlood

Publisher: Academic Press

Published: 2012-08-08

Total Pages: 298

ISBN-13: 0123858720

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In the biomedical sciences, the confocal laser scanning microscope (CLSM) has become the instrument of choice for producing high-resolution images and 3D reconstruction, breaking the barriers of conventional optical microscopy. Wouterlood (anatomy, VU U. Medical Center, Amsterdam, the Netherlands) introduces the confocal principle which eliminates out-of-focus haze, its components, and relevant equations. International scientists explain the principles and related methods of stimulated emission depletion (SRED), single molecule localization, and coherent anti-Stokes Raman (CARS) microscopy; labeling approaches; preparation of samples for imaging; and applications of, and developments in, this new wave of imaging, e.g., visualization of neuronal networks, DNA, and myelin. The text includes color and b&w images, and referral to an online CLSM simulator. Academic Press is an imprint of Elsevier. Annotation ©2013 Book News, Inc., Portland, OR (booknews.com).


Imaging in Neuroscience and Development

Imaging in Neuroscience and Development

Author: Rafael Yuste

Publisher: CSHL Press

Published: 2005

Total Pages: 813

ISBN-13: 0879696923

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As imaging studies have continued to expand in scope and sophistication, this new edition of the highly successful and well–received Imaging Neurons: A Laboratory Manualhas expanded to include development, with over twenty new chapters on such topics as MRI microscopy, imaging early developmental events, and labeling single neurons. Chapters on FRET, FCS/ICS, FRAP, hyperresolution microscopy, single molecule imaging, imaging with quantum dots, and imaging gene expression are included. With over forty full chapters, the manual also includes over forty sections of protocols for imaging techniques.


Caenorhabditis Elegans

Caenorhabditis Elegans

Author: Henry F. Epstein

Publisher: Academic Press

Published: 1995

Total Pages: 687

ISBN-13: 0125641494

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The first of its kind, this laboratory handbook emphasizes diverse methods and technologies needed to investigate C. elegans, both as an integrated organism and as a model system for research inquiries in cell, developmental, and molecular biology, as well as in genetics and pharmacology. Four primary sections--Genetic and Culture Methods, Neurobiology, Cell and Molecular Biology, and Genomics and Informatics--reflect the cross-disciplinary nature of C. elegans research. Because C. elegans is a simple and malleable organism with a small genome and few cell types, it provides an elegant demonstr.


From Photon to Neuron

From Photon to Neuron

Author: Philip Nelson

Publisher: Princeton University Press

Published: 2017-05-09

Total Pages: 512

ISBN-13: 1400885485

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A richly illustrated undergraduate textbook on the physics and biology of light Students in the physical and life sciences, and in engineering, need to know about the physics and biology of light. Recently, it has become increasingly clear that an understanding of the quantum nature of light is essential, both for the latest imaging technologies and to advance our knowledge of fundamental life processes, such as photosynthesis and human vision. From Photon to Neuron provides undergraduates with an accessible introduction to the physics of light and offers a unified view of a broad range of optical and biological phenomena. Along the way, this richly illustrated textbook builds the necessary background in neuroscience, photochemistry, and other disciplines, with applications to optogenetics, superresolution microscopy, the single-photon response of individual photoreceptor cells, and more. With its integrated approach, From Photon to Neuron can be used as the basis for interdisciplinary courses in physics, biophysics, sensory neuroscience, biophotonics, bioengineering, or nanotechnology. The goal is always for students to gain the fluency needed to derive every result for themselves, so the book includes a wealth of exercises, including many that guide students to create computer-based solutions. Supplementary online materials include real experimental data to use with the exercises. Assumes familiarity with first-year undergraduate physics and the corresponding math Overlaps the goals of the MCAT, which now includes data-based and statistical reasoning Advanced chapters and sections also make the book suitable for graduate courses An Instructor's Guide and illustration package is available to professors


When I'm 64

When I'm 64

Author: National Research Council

Publisher: National Academies Press

Published: 2006-02-13

Total Pages: 280

ISBN-13: 0309164915

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By 2030 there will be about 70 million people in the United States who are older than 64. Approximately 26 percent of these will be racial and ethnic minorities. Overall, the older population will be more diverse and better educated than their earlier cohorts. The range of late-life outcomes is very dramatic with old age being a significantly different experience for financially secure and well-educated people than for poor and uneducated people. The early mission of behavioral science research focused on identifying problems of older adults, such as isolation, caregiving, and dementia. Today, the field of gerontology is more interdisciplinary. When I'm 64 examines how individual and social behavior play a role in understanding diverse outcomes in old age. It also explores the implications of an aging workforce on the economy. The book recommends that the National Institute on Aging focus its research support in social, personality, and life-span psychology in four areas: motivation and behavioral change; socioemotional influences on decision-making; the influence of social engagement on cognition; and the effects of stereotypes on self and others. When I'm 64 is a useful resource for policymakers, researchers and medical professionals.


Imaging the Brain with Optical Methods

Imaging the Brain with Optical Methods

Author: Anna W. Roe

Publisher: Springer Science & Business Media

Published: 2009-11-11

Total Pages: 271

ISBN-13: 1441904522

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Monitoring brain function with light in vivo has become a reality. The technology 33 of detecting and interpreting patterns of reflected light has reached a degree of 34 maturity that now permits high spatial and temporal resolution visualization at both 35 the systems and cellular levels. There now exist several optical imaging methodolo- 36 gies, based on either hemodynamic changes in nervous tissue or neurally induced 37 light scattering changes, that can be used to measure ongoing activity in the brain. 38 These include the techniques of intrinsic signal optical imaging, near-infrared optical 39 imaging, fast optical imaging based on scattered light, optical imaging with voltage 40 sensitive dyes, and two-photon imaging of hemodynamic signals. The purpose of 41 this volume is to capture some of the latest applications of these methodologies to 42 the study of cerebral cortical function. 43 This volume begins with an overview and history of optical imaging and its use 44 in the study of brain function. Several chapters are devoted to the method of intrin- 45 sic signal optical imaging, a method used to record the minute changes in optical 46 absorption due to hemodynamic changes that accompanies cortical activity. Since the 47 detected hemodynamic changes are highly localized, this method has excellent 48 spatial resolution (50–100 μm ), a resolution sufficient for visualization of fundamen- 49 tal modules of cerebral cortical function.


Casting Light on the Dark Side of Brain Imaging

Casting Light on the Dark Side of Brain Imaging

Author: Amir Raz

Publisher: Academic Press

Published: 2019-02-15

Total Pages: 206

ISBN-13: 0128163097

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Most people find colorful brain scans highly compelling—and yet, many experts don't. This discrepancy begs the question: What can we learn from neuroimaging? Is brain information useful in fields such as psychiatry, law, or education? How do neuroscientists create brain activation maps and why do we admire them? Casting Light on The Dark Side of Brain Imaging tackles these questions through a critical and constructive lens—separating fruitful science from misleading neuro-babble. In a breezy writing style accessible to a wide readership, experts from across the brain sciences offer their uncensored thoughts to help advance brain research and debunk the craze for reductionist, headline-grabbing neuroscience. This collection of short, enlightening essays is suitable for anyone interested in brain science, from students to professionals. Together, we take a hard look at the science behind brain imaging and outline why this technique remains promising despite its seldom-discussed shortcomings. - Challenges the tendency toward neuro-reductionism - Deconstructs hype through a critical yet constructive lens - Unveils the nature of brain imaging data - Explores emerging brain technologies and future directions - Features a non-technical and accessible writing style


Advanced Optical Methods for Brain Imaging

Advanced Optical Methods for Brain Imaging

Author: Fu-Jen Kao

Publisher: Springer

Published: 2018-05-14

Total Pages: 342

ISBN-13: 9811090203

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This book highlights the rapidly developing field of advanced optical methods for structural and functional brain imaging. As is known, the brain is the most poorly understood organ of a living body. It is indeed the most complex structure in the known universe and, thus, mapping of the brain has become one of the most exciting frontlines of contemporary research. Starting from the fundamentals of the brain, neurons and synapses, this book presents a streamlined and focused coverage of the core principles, theoretical and experimental approaches, and state-of-the-art applications of most of the currently used imaging methods in brain research. It presents contributions from international leaders on different photonics-based brain imaging modalities and techniques. Included are comprehensive descriptions of many of the technology driven spectacular advances made over the past few years that have allowed novel insights of the structural and functional details of neurons. The book is targeted at researchers, engineers and scientists who are working in the field of brain imaging, neuroscience and connectomics. Although this book is not intended to serve as a textbook, it will appeal to undergraduate students engaged in the specialization of brain imaging.