This volume aims to provide protocols on a wide range of biochemical methods, analytical approaches, and bioinformatics tools developed to analyze the proteome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Proteomics: Methods and Protocols aims to ensure successful results in the further study of this vital field.
"This Report extends the concepts and approaches discussed in NCRP Report No. 171 and Commentary No. 24 to further reduce uncertainty in radiation risk assessments at low doses and low dose rates, thereby enhancing the bases for radiation protection guidance"--
This fully updated and enhanced third edition offers a highly practical, application-based review of the biological basis of radiation oncology and the clinical efficacy of radiation therapy. Revised edition of the classic reference in radiation oncology from Dr. C.C. Wang, whose practical approach to clinical application was legendary Includes the latest developments in the field: intensity modulated radiation therapy (IMRT), image guided radiation therapy, and particle beam therapy Includes two brand new chapters Palliative Radiotherapy, and Statistics in Radiation Oncology Features a vibrant and extremely comprehensive head and neck section Provides immediately applicable treatment algorithms for each tumor
Driven by the widespread growth of proteomic practices, protein separation techniques have been refined to minimize variability, optimize particular applications, and adapt to user preferences in the analysis of proteins. Separation Methods in Proteomics provides a comprehensive examination of all major separation techniques for proteomic
In recent times there has been an explosive expansion of new imaging methodologies that are capable of visualizing specific populations of cells and molecular events in vivo. Vital imaging enhances our ability to study animal models of human development and disease, such as cancers, cardiovascular disease, diabetes, and Alzheimer's. Furthermore, non-invasive imaging may ultimately be useful for monitoring new generations of clinical molecular and cellular therapeutics, such as those utilizing viral vectors and stem cells. These new capabilities have been facilitated by the development of new imaging probes or reagents that target specific cell types, are chemically responsive to physiology, or are responsive to the presence of specific molecules, such as nucleic acids or enzymes. This volume provides an introduction to some of the most exciting methods and applications of emerging non-invasive imaging technologies using magnetic resonance imaging (MRI), positron emission tomography (PET), and various biophotonic approaches. Highlighted, are recent developments in reagent design that impart unique abilities to these imaging modalities to elucidate biological processes in vivo. * Includes 9 chapters by expert researchers in the field of imaging * Introduces new methods and applications of non-invasive imaging technologies * Covers ermerging topics in imaging such as in vivo cell cancer cells, imaging of autoimmune diseases, and maganetic resonance imaging
