High Resolution Separation and Analysis of Biological Macromolecules
High Resolution Separation and Analysis of Biological Macromolecules

Author: Barry L. Karger

Publisher: Elsevier

Published: 1996

Total Pages: 664

ISBN-13: 9780121821715

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The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 260 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. Key Features* Liquid chromatography* Electrophoresis* Mass spectrometry.

High Resolution Separation and Analysis of Biological Macromolecules, Part B: Applications
High Resolution Separation and Analysis of Biological Macromolecules, Part B: Applications

Author: Barry L. Karger

Publisher: Academic Press

Published: 1996-07-18

Total Pages: 604

ISBN-13:

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The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 260 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. Liquid chromatography Electrophoresis Mass spectrometry

Biothermodynamics
Biothermodynamics

Author: Michael L. Johnson

Publisher: Academic Press

Published: 2011

Total Pages: 409

ISBN-13: 0123812682

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In the last several years there has been an explosion in the ability of biologists, molecular biologists and biochemists to collect vast amounts of data on their systems. This volume presents sophisticated methods for estimating the thermodynamic parameters of specific protein-protein, protein-DNA and small molecule interactions.

Liposomes, Part B
Liposomes, Part B

Author: Nejat Duzgunes

Publisher: Elsevier

Published: 2003-11-19

Total Pages: 517

ISBN-13: 008049711X

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Liposomes are cellular structures made up of lipid molecules. Important as a cellular model in the study of basic biology, liposomes are also used in clinical applications such as drug delivery and virus studies. - Liposomes in Biochemistry - Liposomes in Molecular Cell Biology - Liposomes in Molecular Virology

Guide to Yeast Genetics and Molecular Biology
Guide to Yeast Genetics and Molecular Biology

Author:

Publisher: Gulf Professional Publishing

Published: 2004-05-14

Total Pages: 961

ISBN-13: 0080575137

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Guide to Yeast Genetics and Molecular Biology presents, for the first time, a comprehensive compilation of the protocols and procedures that have made Saccharomyces cerevisiae such a facile system for all researchers in molecular and cell biology. Whether you are an established yeast biologist or a newcomer to the field, this volume contains all the up-to-date methods you will need to study "Your Favorite Gene" in yeast.Key Features* Basic Methods in Yeast Genetics* Physical and genetic mapping* Making and recovering mutants* Cloning and Recombinant DNA Methods* High-efficiency transformation* Preparation of yeast artificial chromosome vectors* Basic Methods of Cell Biology* Immunomicroscopy* Protein targeting assays* Biochemistry of Gene Expression* Vectors for regulated expression* Isolation of labeled and unlabeled DNA, RNA, and protein

RNA Turnover in Eukaryotes: Nucleases, Pathways and Analysis of mRNA Decay
RNA Turnover in Eukaryotes: Nucleases, Pathways and Analysis of mRNA Decay

Author: Lynne E. Maquat

Publisher: Academic Press

Published: 2009-01-30

Total Pages: 661

ISBN-13: 0080922074

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Specific complexes of protein and RNA carry out many essential biological functions, including RNA processing, RNA turnover, RNA folding, as well as the translation of genetic information from mRNA into protein sequences. Messenger RNA (mRNA) decay is now emerging as an important control point and a major contributor to gene expression. Continuing identification of the protein factors and cofactors, and mRNA instability elements responsible for mRNA decay allow researchers to build a comprehensive picture of the highly orchestrated processes involved in mRNA decay and its regulation. - Covers the nonsense-mediated mRNA decay (NMD) or mRNA surveillance pathway - Expert researchers introduce the most advanced technologies and techniques to identify mRNA processing, transport, localization and turnover, which are central to the process of gene expression - Offers step-by-step lab instructions, including necessary equipment and reagents

Hydrogen Peroxide and Cell Signaling, Part B
Hydrogen Peroxide and Cell Signaling, Part B

Author:

Publisher: Academic Press

Published: 2013-06-29

Total Pages: 383

ISBN-13: 0124055419

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This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This is the second of three volumes on hydrogen peroxide and cell signaling, and includes chapters on such topics as the cellular steady-state of H2O2, evaluating peroxiredoxin sensitivity towards inactivation by peroxide substrates, and peroxiredoxins as preferential targets in H2O2-induced signaling. - Continues the legacy of this premier serial with quality chapters authored by leaders in the field - Covers hydrogen peroxide and cell signaling - Contains chapters on such topics as the cellular steady-state of H2O2, evaluating peroxiredoxin sensitivity towards inactivation by peroxide substrates, and peroxiredoxins as preferential targets in H2O2-induced signaling

Oxygen Biology and Hypoxia
Oxygen Biology and Hypoxia

Author:

Publisher: Academic Press

Published: 2007-11-08

Total Pages: 592

ISBN-13: 0080554865

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For over fifty years the Methods in Enzymology series has been the critically acclaimed laboratory standard and one of the most respected publications in the field of biochemistry. The highly relevant material makes it an essential publication for researchers in all fields of life and related sciences. This volume features articles on the topic of oxygen biology and hypoxia.

Single Molecule Tools, Part A: Fluorescence Based Approaches
Single Molecule Tools, Part A: Fluorescence Based Approaches

Author:

Publisher: Academic Press

Published: 2010-08-17

Total Pages: 559

ISBN-13: 008095927X

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Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and "just look at the thing" as anticipated by the visionary Richard Feynman already half a century ago. Single Molecule Tools, Part A: Fluorescence Based Approaches captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule tools. - Includes time-tested core methods and new innovations applicable to any researcher employing single molecule tools - Methods included are useful to both established researchers and newcomers to the field - Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines

Measuring Biological Responses with Automated Microscopy
Measuring Biological Responses with Automated Microscopy

Author:

Publisher: Elsevier

Published: 2006-11-02

Total Pages: 736

ISBN-13: 0080468985

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The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences.