Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition
Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition

Author: Leland J. Cseke

Publisher: CRC Press

Published: 2003-11-24

Total Pages: 604

ISBN-13: 9780849308154

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Since the publication of the best-selling Handbook of Molecular and Cellular Methods in Biology and Medicine, the field of biology has experienced several milestones. Genome sequencing of higher eukaryotes has progressed at an unprecedented speed. Starting with baker's yeast (Saccharomyces cerevisiae), organisms sequenced now include human (Homo sapiens), model crucifer (Arabidopsis thaliana), and rice (Oryza sativa). The invention of DNA microarray technology and advances in bioinformatics have generated vast amounts of genomic data. Reflecting these revolutionary advances Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition documents conventional and modern approaches to tackle scientific research in the post-genomics era. Maintaining the step-by-step format that popularized the first edition, each chapter provides the principles behind the featured method, a detailed description of each protocol, applications of the protocol to different systems, and references for further study. Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition now includes: New protocols in all chapters, including alternative protocols In vitro transcription methods Analysis of DNA sequences New bioseparation techniques New chapters covering: mRNA differential display Inhibition of gene expression In situ hybridization (Localization of gene expression) Combinatorial techniques Computational data mining methods applied to combinatorial chemistry libraries With this book at hand, researchers, teachers, and students can understand and utilize the major techniques and methods currently employed in cellular and molecular biology.

Handbook of Molecular and Cellular Methods in Biology and Medicine
Handbook of Molecular and Cellular Methods in Biology and Medicine

Author: Leland J. Cseke

Publisher: CRC Press

Published: 2003-11-24

Total Pages: 598

ISBN-13: 1420041711

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Since the publication of the best-selling Handbook of Molecular and Cellular Methods in Biology and Medicine, the field of biology has experienced several milestones. Genome sequencing of higher eukaryotes has progressed at an unprecedented speed. Starting with baker's yeast (Saccharomyces cerevisiae), organisms sequenced now include human (Homo sa

Molecular Biomethods Handbook
Molecular Biomethods Handbook

Author: John M. Walker

Publisher: Springer Science & Business Media

Published: 2008-11-04

Total Pages: 1105

ISBN-13: 1603273751

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Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. This book expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Written by a well-established panel of research scientists, the book provides an up-to-date collection of methods used regularly in the authors’ own research programs.

Handbook of Molecular and Cellular Methods in Biology and Medicine
Handbook of Molecular and Cellular Methods in Biology and Medicine

Author: Leland J. Cseke

Publisher: CRC Press

Published: 2016-04-19

Total Pages: 723

ISBN-13: 1439881952

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Several milestones in biology have been achieved since the first publication of the Handbook of Molecular and Cellular Methods in Biology and Medicine. This is true particularly with respect to genome-level sequencing of higher eukaryotes, the invention of DNA microarray technology, advances in bioinformatics, and the development of RNAi technology

Concise Dictionary of Biomedicine and Molecular Biology
Concise Dictionary of Biomedicine and Molecular Biology

Author: Pei-Show Juo

Publisher: CRC Press

Published: 2001-12-21

Total Pages: 1163

ISBN-13: 1420041304

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Rapid advances in science, medicine, and molecular biology have created a large amount of new information on biomedicine and molecular biology. Keeping up with the latest information can become a cumbersome task for professionals and students working in these fields. Updated to include new terminology and accurate characterizations of previously ex

Advanced Methods in Cellular Immunology
Advanced Methods in Cellular Immunology

Author: Rafael Fernandez-Botran

Publisher: CRC Press

Published: 2000-05-26

Total Pages: 177

ISBN-13: 1420039237

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Immunologists as well investigators in other disciplines may often use protocols involving the isolation, cultures and characterization of different types of leukocytes. Advanced Methods in Cellular Immunology is a collection of techniques in an easy-to-use format. Each chapter provides readers with related program information, a step-by-step de

Handbook of Molecular and Cellular Methods in Biology and Medicine
Handbook of Molecular and Cellular Methods in Biology and Medicine

Author: Donghern Kim

Publisher: CRC Press

Published: 1995-05-15

Total Pages: 496

ISBN-13: 9780849325113

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Many of the available books in cellular and molecular biology fail to explain the principles and methods described. Handbook of Molecular and Cellular Methods in Biology and Medicine fills in these gaps. Not only do the authors describe the many methods and techniques most commonly used, but they also discuss why you would use each particular method. This handbook covers a wide range of techniques and methods that have been developed and extensively tested. Described in a logical framework of 21 chapters, methods include isolation and purification of DNA, RNA, and proteins; preparation of hybridization probes; electrophoresis and blotting techniques; construction of cDNA and genomic DNA libraries; DNA sequencing; PCR technology; DNA recombination and mutagenesis; transcription in vitro and translation in vitro; gene transfer and analysis of gene expression in transgenic animals and plants; cell and tissue cultures; and microscopy, as well as bioseparation techniques and preparation of monoclonal and polyclonal antibodies. The authors provide descriptive illustrations for each method. Step-by-step instructions allow you to use this book in the lab while performing the various techniques. Each chapter provides the principles behind the method, easy-to-follow instructions, the reagents and materials required, and references for further study. Many chapters also include a troubleshooting guide, cautioning what to avoid and indicating steps that require extra care. Every biologist needs this book on their lab bench!

Human Cell Culture Protocols
Human Cell Culture Protocols

Author: Joanna Picot

Publisher: Springer Science & Business Media

Published: 2008-02-02

Total Pages: 357

ISBN-13: 1592598617

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A thoroughly revised and updated collection readily reproducible techniques for culturing human cells. This new edition includes a wide range of human cell types relevant to human disease and new chapters on fibroblasts, Schwann cells, gastric and colonic epithelial cells, and parathyroid cells. The protocols follow the successful Methods in Molecular MedicineTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.