Gas Chromatography of Steroids in Biological Fluids

Gas Chromatography of Steroids in Biological Fluids

Author: Mortimer B. Lipsett

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 317

ISBN-13: 1468406914

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During the past decade we have witnessed a revolution in analytical methods. The development of vapor phase chromatography for the separation and analysis of classes of substances ranging from metals and gases to a wide variety of organic materials has been one of the most exciting of these new techniques. Gas-liquid chromatography for the measurement of steroids is particularly significant for endocrinologists and reports during the past several years have demonstrated its usefulness. Because of the growing interest in this method, a committee of the Endocrinology Study Section composed of Drs. R. M. Dodson, Seymour Lieberman, Hilton A. Salhanick, and Ralph E. Peterson, felt that the time was propitious to hold this Workshop and it is on their behalf that I welcome you. We hope to obtain enough data during these sessions so that those attending this conference and those who may read the proceedings will be able to make an informed judgement about the usefulness of gas-liquid chromatography for the analysis of steroids in biological fluids. Thus, I hope that there will be adequate documentation of the reliability of the methods as well as a comparison of the advantages and disadvantages of this analytical method with other classical methods. If we can do this, this Workshop will provide a significant base of practical considerations about gas chromatographic analytic techniques. I would like to thank Drs. T. F. Gallagher, H. Wilson, H. Salhanick and L. Engel for agreeing to serve as Chairmen of the sessions.


Hormone Assays in Biological Fluids

Hormone Assays in Biological Fluids

Author: Michael J. Wheeler

Publisher: Springer Science & Business Media

Published: 2008-02-04

Total Pages: 259

ISBN-13: 1592599869

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Expert researchers who have developed and applied significant new assays describe in step-by-step detail a variety of methods for measuring a broad variety of hormones, related peptides, and synthetic steroids in various biological fluids. The hormones measured range from glucocorticoids in biological fluids, urinary steroids, aldosterone in blood, and plasma renin activity, to gut hormones in plasma, melatonin, prolactin, 6-sulfatoxymelatonin, and androgens in blood, saliva, and hair. The emphasis is on noncommercial assays so that investigators can set up novel methods suited to their special needs. Commercial assays are also described for comparative purposes. Tutorials on radioimmunoassay, gas chromatography-mass spectrometry, high-performance liquid chromatography, and PCR techniques help the reader to choose the best method for his or her purpose.


Laboratory Guide to the Methods in Biochemical Genetics

Laboratory Guide to the Methods in Biochemical Genetics

Author: Nenad Blau

Publisher: Springer Science & Business Media

Published: 2008-05-31

Total Pages: 860

ISBN-13: 3540766987

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This manual deals specifically with laboratory approaches to diagnosing inborn errors of metabolism. The key feature is that each chapter is sufficiently detailed so that any individual can adopt the described method into their own respective laboratory.


Hormonal Steroids

Hormonal Steroids

Author: V. H. T. James

Publisher: Elsevier

Published: 2013-10-22

Total Pages: 1098

ISBN-13: 1483158950

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Hormonal Steroids contains the plenary and symposium lectures delivered to the Fifth International Congress on Hormonal Steroids held at New Delhi on October 30 to November 4, 1978. The plenary lectures include biochemical actions of trophic hormones and steroids on steroid production and spermatogenesis in testes; chemical reactions of steroids which imitate the selectivity of enzymatic transformations; and human chorionic gonadotrophin and ovarian and placental steroidogenesis. Symposia papers comprise of water-soluble steroidal anesthetics; quality control of radioimmunoassay of hormones in reproductive physiology; automation of steroid radioimmunoassay for clinical and research purposes; non-radioisotopic homogeneous steroid immunoassays; mechanisms involved in the regulation of steroid receptor levels; and metabolic effects of corticosteroid therapy in post-menopausal women.


Current Catalog

Current Catalog

Author: National Library of Medicine (U.S.)

Publisher:

Published: 1966

Total Pages: 1076

ISBN-13:

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Includes subject section, name section, and 1968-1970, technical reports.