This substantially updated edition now in full colour provides key techniques used when working with fungal and fungal-like plant pathogens. As a practical manual it also deals with disease recognition, detection and identification of fungi, plus methods to characterise and curate fungi and handle them under quarantine and quality assurance systems. Fungal Plant Pathogens: Applied Techniques, 2nd edition provides a valuable guide to investigating fungal plant diseases and interpreting laboratory findings for postgraduate and advanced undergraduate students, extension plant pathologists, consultants and advisers in agriculture, forestry and horticulture, and the food supply chain.
Including reproducible laboratory protocols, this guide to fungal pathogens in plants has been written by expert researchers in the field and includes methods now used to study them, including techniques for model systems such as Arabidopsis thaliana.
This treatise is focused on early aspects of fungal pathogenesis in plant and animal hosts. Our aim in choosing the topics and contributors was to demonstrate common approaches to studies of fungal-plant and fungal-animal interactions, particularly at the biochemical and molecular Ievels. For example, the initial events of adh«sion of fungal spores to the exposed surface tissues of the host are essential for subsequent invasion of the plant or animal and establishment of pathogenesis. A point of consensus among investigators who have directed their attention to such events in plants, insects, and vertebrates isthat spore adhesion to the host cuticle or epithelium is more than a simple binding event. lt is a complex and potentially pivotal process in fungal-plant interactions which "may involve the secretion of ftuids that prepare the infection court for the development of morphological stages of the germling" and subsequent invasion of the host (Nicholson and Epstein, Chapter 1). The attachment of the fungal propagule to the arthropod cuticle is also "mediated by the chemical components present on the outer layer of the spore wall and the epicuticle . . . . Initial attachment may be reinforced further by either the active secretion of adhesive materials or the modification of spore wall materiallocated at the [fungal spore arthropod] cuticle interface (Boucias and Pendland, Chapter 5).
Fungal diseases have contributed to death and disability in humans, triggered global wildlife extinctions and population declines, devastated agricultural crops, and altered forest ecosystem dynamics. Despite the extensive influence of fungi on health and economic well-being, the threats posed by emerging fungal pathogens to life on Earth are often underappreciated and poorly understood. On December 14 and 15, 2010, the IOM's Forum on Microbial Threats hosted a public workshop to explore the scientific and policy dimensions associated with the causes and consequences of emerging fungal diseases.
Up-to-date, accurate information on recent developments in crop protection! Fungal Disease Resistance in Plants: Biochemistry, Molecular Biology, and Genetic Engineering presents the latest developments in crop protection from fungal infection. Leading experts in botany, plant breeding, and plant pathology contribute their knowledge to help reduce and possibly prevent new outbreaks of devastating crop epidemics caused by fungi. With exciting new advances in molecular biology, biochemistry, and genetic engineering, this informative book will help researchers, professors, and students further their understanding of plant defenses. Fungal Disease Resistance in Plants is your guide to understanding the various barriers that plants have developed through evolution and adaptation to protect themselves from invading fungal pathogens. Defenses include physical barriers such as thick cell walls and chemical compounds expressed by the plant when attacked. Still other plants have acquired proteins that play an important role in defense. This book discusses these evolutionary traits and introduces new scientific techniques to engineer resistance in plants that have no built-in protection. Fungal Disease Resistance in Plants explores: cellular expression of resistance to fungal pathogens the hypersensitive response and its role in disease resistance induced plant resistance to fungal pathogens—mechanisms and practical applications pathogenesis-related proteins and their roles in resistance to fungal pathogens signal transduction—plant networks, delivery, and response to fungal infection fungus genes as they relate to disease susceptibility and resistance Without intense research and scientific study, catastrophic harvest failures due to fungal diseases could cause food shortages, human and animal poisonings, and economic loss throughout the world. Augmented with tables, figures, and extensive references, this state-of-the-art source of research material is valuable for scientists and researchers in universities, private organizations, government institutions, and agricultural organizations interested in plant defenses and future crop preservation.
The variety of the mycological world is far greater than most people imagine. Tens of thousands of fungal species have been described and many more are known only from the abundance of their genes in soil and water. Fungi are hugely important as agents of wood decay in forests, and, as parasites, they have caused the deaths of millions of people by ravaging crops and reshaping natural ecosystems. Fungi perform a variety of essential functions in ecosystems, and are important to both agriculture and biotechnology. Their importance is now becoming better appreciated among scientists, though there is much still to be understood concerning their taxonomy and evolution. This Very Short Introduction highlights the variety and extraordinary natures of fungi, revealing the remarkable facts of fungal biology and the global significance of these enchanting organisms. ABOUT THE SERIES: The Very Short Introductions series from Oxford University Press contains hundreds of titles in almost every subject area. These pocket-sized books are the perfect way to get ahead in a new subject quickly. Our expert authors combine facts, analysis, perspective, new ideas, and enthusiasm to make interesting and challenging topics highly readable.
Laboratory Protocols in Fungal Biology presents the latest techniques in fungal biology. This book analyzes information derived through real experiments, and focuses on cutting edge techniques in the field. The book comprises 57 chapters contributed from internationally recognised scientists and researchers. Experts in the field have provided up-to-date protocols covering a range of frequently used methods in fungal biology. Almost all important methods available in the area of fungal biology viz. taxonomic keys in fungi; histopathological and microscopy techniques; proteomics methods; genomics methods; industrial applications and related techniques; and bioinformatics tools in fungi are covered and complied in one book. Chapters include introductions to their respective topics, list of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting. Each chapter is self-contained and written in a style that enables the reader to progress from elementary concepts to advanced research techniques. Laboratory Protocols in Fungal Biology is a valuable tool for both beginner research workers and experienced professionals. Coming Soon in the Fungal Biology series: Goyal, Manoharachary / Future Challenges in Crop Protection Against Fungal Pathogens Martín, García-Estrada, Zeilinger / Biosynthesis and Molecular Genetics of Fungal Secondary Metabolites Zeilinger, Martín, García-Estrada / Biosynthesis and Molecular Genetics of Fungal Secondary Metabolites, Volume 2 van den Berg, Maruthachalam / Genetic Transformation Systems in Fungi Schmoll, Dattenbock / Gene Expression Systems in Fungi Dahms / Advanced Microscopy in Mycology
Morphological, biological, biochemical and physiological characteristics have been used for the detection, identification and differentiation of fungal pathogens up to species level. Tests based on biological characteristics are less consistent. Immunoassays have been shown to be effective in detecting fungal pathogens present in plants and environmental samples. Development of monoclonal antibody technology has greatly enhanced the sensitivity and specificity of detection, identification and differentiation of fungal species and varieties/strains. Nucleic acid-based techniques involving hybridization with or amplification of unique DNA have provided results rapidly and reliably. Presentation of a large number of protocols is a unique feature of this volume.
Dramatic progress in molecular biology and genetic engineering has recently produced an unparalleled wealth of information on the mechanisms of plant and pathogen interactions at the cellular and molecular levels. Completely revised and expanded, Fungal Pathogenesis in Plants and Crops: Molecular Biology and Host Defense Mechanisms, Second Edition
