This book covering the basics and the state-of-the-art of cryobiology and its applications emphasizes the underlying physical phenomena. It includes a comprehensive glossary and appendices for deeper exploration into special issues.
In addition to outlining the fundamental principles associated with the conservation of biological resources, freeze-drying and cryopreservation, this text is a compilation of cryptopreservation and freeze-drying methodologies applicable to different biological materiels, developed by expert laboratories.
The specialty of fertility preservation offers patients with cancer, who are rendered infertile by chemo- and radiotherapy, the opportunity to realize their reproductive potential. This gold-standard publication defines the specialty. The full range of techniques and scientific concepts is covered in detail, and the author team includes many of the world's leading experts in the field. The book opens with introductions to fertility preservation in both cancer and non-cancer patients, followed by cancer biology, epidemiology and treatment, and reproductive biology and cryobiology. Subsequent sections cover fertility preservation strategies in males and females, including medical/surgical procedures, ART, cryopreservation and transplantation of both ovarian tissue and the whole ovary, and in-vitro follicle growth and maturation. Concluding chapters address future technologies, as well as ethical, legal and religious issues. Richly illustrated throughout, this is a key resource for all clinicians specializing in reproductive medicine, gynecology, oncology, hematology, endocrinology and infertility.
While it is barely 50 years since the first reliable reports of the recovery of living cells frozen to cryogenic temperatures, there has been tremendous growth in the use of cryobiology in medicine, agriculture, horticulture, forestry, and the conservation of endangered or economically important species. As the first major text on cryobiolog
Reproductive technologies to assist in both human conception and animal breeding are increasingly in demand. These technologies, along with the advent of tissue engineering, have propelled the challenges of tissue collection, preservation, and banking to the research forefront. Using examples drawn from reproductive technologies, Reproductive Tissue Banking presents the scientific principles underlying tissue banking. These examples serve as models for the technology of banking other living tissues, including blood, bone marrow, cornea, and skin. In discussing research emerging from their laboratories and those of others, the authors meld fundamentals of biology, chemistry, and physics with the latest discoveries in the field to give the reader profound insight into research directions and ethical considerations crucial to the advancement of tissue banking.With its emphasis on human applications and concerns, this book provides a valuable supplement to short courses on tissue preservation and tissue engineering. Researchers in reproductive medicine, animal and veterinary science, and cryobiology will find this book, with its extensive bibliography, a very handy reference.* * Written by leading international researchers* Provides insightful discussions on reproductive tissue banking* Presents comprehensive citations to relevant literature, both current and historic* Discusses in vitro preservation of spermatozoa, oocytes, embryos, and gonadal tissues of mammals* Contains coverage of ethical considerations from a discussion of the splitting of embryos to an exploration of the protection of biodiversity
Oocyte cryopreservation entails important potential advantages for humanIVF, offering a less ethically disputable alternative to embryo cryopreservation,simplifying and making safer oocyte donation, and giving an opportunity forfertility preservation to women at risk of premature ovarian failure as an effectof genetic factors or chemo- or radiother
Cryopreservation has many biotechnological applications in different fields. This has led to an increase in importance of cryobiology as a science that examines the effect of ultra-low temperatures on cells, tissues, organs and organisms and also the freezability of these structures, while maintaining their viability. Nowadays it is well known that this form of biotechnology can be used to solve a lot of problems such as human infertility, life threatening diseases, preservation of gametes and DNA and also biodiversity conservation. Cryopreservation Biotechnology in Biomedical and Biological Sciences describes principles and application of cryopreservation biotechnology in different research areas and includes seven chapters that have been written by experts in their research fields. The chapters included in this book are thought to improve the current understanding of the different areas of using cryopreservation biotechnology.
Cryopreservation - Current Advances and Evaluations sheds light on storage of cells at subzero temperatures while ensuring that biological functionality is not compromised. Cryopreservation presents a perfect technique by which life can be preserved for posterity. However, there are many challenges to overcome and questions to answer, such as: Are organisms and metabolic systems functioning normally after cooling and thawing? This book provides comprehensive information on cryopreservation with a particular focus on cryoprotectant agents (CPAs). CPAs prevent ice from forming on cryogenically preserved cells, tissues, and organs, but can become toxic at high concentrations. As such, more research is needed to determine their precise mechanisms of action and to develop potential new CPAs that will not compromise the biology of cells. This book is an attempt in this direction.
This practical, extensively illustrated handbook covers the procedures that are undertaken in andrology and ART laboratories to analyse and assess male-factor infertility, and to prepare spermatozoa for use in assisted conception therapy. The content is presented as brief, authoritative overviews of the relevant biological background for each area, plus detailed, step-by-step descriptions of the relevant analytical procedures. Each technical section includes pertinent quality control considerations, as well as the optimum presentation of results. In addition to the comprehensive 'basic' semen analysis, incorporating careful analysis of sperm morphology, the handbook provides established techniques for the use of computer-aided sperm analysis and sperm functional assessment. Throughout the handbook the interpretation of laboratory results in the clinical context is highlighted, and safe laboratory practice is emphasized. It is an invaluable resource to all scientists and technicians who perform diagnostic testing for male-factor infertility.
Moving rapidly from science fiction to science fact, cryopreservation is an integral part of many research, development, and production processes in industry and academia. The preservation sciences have emerged as an interdisciplinary platform that incorporates the fundamentals of cell and molecular biology, and bioengineering, with the classic met