Next Generation Sequencing

Next Generation Sequencing

Author: Jerzy Kulski

Publisher: BoD – Books on Demand

Published: 2016-01-14

Total Pages: 466

ISBN-13: 9535122401

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Next generation sequencing (NGS) has surpassed the traditional Sanger sequencing method to become the main choice for large-scale, genome-wide sequencing studies with ultra-high-throughput production and a huge reduction in costs. The NGS technologies have had enormous impact on the studies of structural and functional genomics in all the life sciences. In this book, Next Generation Sequencing Advances, Applications and Challenges, the sixteen chapters written by experts cover various aspects of NGS including genomics, transcriptomics and methylomics, the sequencing platforms, and the bioinformatics challenges in processing and analysing huge amounts of sequencing data. Following an overview of the evolution of NGS in the brave new world of omics, the book examines the advances and challenges of NGS applications in basic and applied research on microorganisms, agricultural plants and humans. This book is of value to all who are interested in DNA sequencing and bioinformatics across all fields of the life sciences.


Learning to Learn

Learning to Learn

Author: Sebastian Thrun

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 346

ISBN-13: 1461555299

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Over the past three decades or so, research on machine learning and data mining has led to a wide variety of algorithms that learn general functions from experience. As machine learning is maturing, it has begun to make the successful transition from academic research to various practical applications. Generic techniques such as decision trees and artificial neural networks, for example, are now being used in various commercial and industrial applications. Learning to Learn is an exciting new research direction within machine learning. Similar to traditional machine-learning algorithms, the methods described in Learning to Learn induce general functions from experience. However, the book investigates algorithms that can change the way they generalize, i.e., practice the task of learning itself, and improve on it. To illustrate the utility of learning to learn, it is worthwhile comparing machine learning with human learning. Humans encounter a continual stream of learning tasks. They do not just learn concepts or motor skills, they also learn bias, i.e., they learn how to generalize. As a result, humans are often able to generalize correctly from extremely few examples - often just a single example suffices to teach us a new thing. A deeper understanding of computer programs that improve their ability to learn can have a large practical impact on the field of machine learning and beyond. In recent years, the field has made significant progress towards a theory of learning to learn along with practical new algorithms, some of which led to impressive results in real-world applications. Learning to Learn provides a survey of some of the most exciting new research approaches, written by leading researchers in the field. Its objective is to investigate the utility and feasibility of computer programs that can learn how to learn, both from a practical and a theoretical point of view.


The Mouse Nervous System

The Mouse Nervous System

Author: Charles Watson

Publisher: Academic Press

Published: 2011-11-28

Total Pages: 815

ISBN-13: 0123694973

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The Mouse Nervous System provides a comprehensive account of the central nervous system of the mouse. The book is aimed at molecular biologists who need a book that introduces them to the anatomy of the mouse brain and spinal cord, but also takes them into the relevant details of development and organization of the area they have chosen to study. The Mouse Nervous System offers a wealth of new information for experienced anatomists who work on mice. The book serves as a valuable resource for researchers and graduate students in neuroscience. Systematic consideration of the anatomy and connections of all regions of the brain and spinal cord by the authors of the most cited rodent brain atlases A major section (12 chapters) on functional systems related to motor control, sensation, and behavioral and emotional states A detailed analysis of gene expression during development of the forebrain by Luis Puelles, the leading researcher in this area Full coverage of the role of gene expression during development and the new field of genetic neuroanatomy using site-specific recombinases Examples of the use of mouse models in the study of neurological illness


Gene Network Inference

Gene Network Inference

Author: Alberto Fuente

Publisher: Springer Science & Business Media

Published: 2014-01-03

Total Pages: 135

ISBN-13: 3642451616

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This book presents recent methods for Systems Genetics (SG) data analysis, applying them to a suite of simulated SG benchmark datasets. Each of the chapter authors received the same datasets to evaluate the performance of their method to better understand which algorithms are most useful for obtaining reliable models from SG datasets. The knowledge gained from this benchmarking study will ultimately allow these algorithms to be used with confidence for SG studies e.g. of complex human diseases or food crop improvement. The book is primarily intended for researchers with a background in the life sciences, not for computer scientists or statisticians.


Untranslated Gene Regions and Other Non-coding Elements

Untranslated Gene Regions and Other Non-coding Elements

Author: Lucy W. Barrett

Publisher: Springer Science & Business Media

Published: 2013-06-26

Total Pages: 63

ISBN-13: 3034806795

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There is now compelling evidence that the complexity of higher organisms correlates with the relative amount of non-coding RNA rather than the number of protein-coding genes. Previously dismissed as “junk DNA”, it is the non-coding regions of the genome that are responsible for regulation, facilitating complex temporal and spatial gene expression through the combinatorial effect of numerous mechanisms and interactions working together to fine-tune gene expression. The major regions involved in regulation of a particular gene are the 5’ and 3’ untranslated regions and introns. In addition, pervasive transcription of complex genomes produces a variety of non-coding transcripts that interact with these regions and contribute to regulation. This book discusses recent insights into the regulatory roles of the untranslated gene regions and non-coding RNAs in the control of complex gene expression, as well as the implications of this in terms of organism complexity and evolution.​


In the Light of Evolution

In the Light of Evolution

Author: National Academy of Sciences

Publisher:

Published: 2007

Total Pages: 388

ISBN-13:

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The Arthur M. Sackler Colloquia of the National Academy of Sciences address scientific topics of broad and current interest, cutting across the boundaries of traditional disciplines. Each year, four or five such colloquia are scheduled, typically two days in length and international in scope. Colloquia are organized by a member of the Academy, often with the assistance of an organizing committee, and feature presentations by leading scientists in the field and discussions with a hundred or more researchers with an interest in the topic. Colloquia presentations are recorded and posted on the National Academy of Sciences Sackler colloquia website and published on CD-ROM. These Colloquia are made possible by a generous gift from Mrs. Jill Sackler, in memory of her husband, Arthur M. Sackler.


Regulation of Alternative Splicing

Regulation of Alternative Splicing

Author: Philippe Jeanteur

Publisher: Springer Science & Business Media

Published: 2002-10-21

Total Pages: 272

ISBN-13: 9783540438335

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The discovery in 1977 that genes are split into exons and introns has done away with the one gene - one protein dogma. Indeed, the removal of introns from the primary RNA transcript is not necessarily straightforward since there may be optional pathways leading to different messenger RNAs and consequently to different proteins. Examples of such an alternative splicing mechanism cover all fields of biology. Moreover, there are plenty of occurrences where deviant splicing can have pathological effects. Despite the high number of specific cases of alternative splicing, it was not until recently that the generality and extent of this phenomenon was fully appreciated. A superficial reading of the preliminary sequence of the human genome published in 2001 led to the surprising, and even deceiving to many scientists, low number of genes (around 32,000) which contrasted with the much higher figure around 150,000 which was previously envisioned. Attempts to make a global assessment of the use of alternative splicing are recent and rely essentially on the comparison of genomic mRNA and EST sequences as reviewed by Thanaraj and Stamm in the first chapter of this volume. Most recent estimates suggest that 40-60% of human genes might be alternatively spliced, as opposed to about 22% for C. elegans.


High Density Lipoproteins

High Density Lipoproteins

Author: Arnold von Eckardstein

Publisher: Springer

Published: 2015-01-13

Total Pages: 0

ISBN-13: 9783319096643

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In this Handbook of Experimental Pharmacology on “High Density Lipoproteins – from biological understanding to clinical exploitation” contributing authors (members of COST Action BM0904/HDLnet) summarize in more than 20 chapters our current knowledge on the structure, function, metabolism and regulation of HDL in health and several diseases as well as the status of past and ongoing attempts of therapeutic exploitation. The book is of interest to researchers in academia and industry focusing on lipoprotein metabolism, cardiovascular diseases and immunology as well as clinical pharmacologists, cardiologists, diabetologists, nephrologists and other clinicians interested in metabolic or inflammatory diseases.


Computational Methods for Single-Cell Data Analysis

Computational Methods for Single-Cell Data Analysis

Author: Guo-Cheng Yuan

Publisher: Humana Press

Published: 2019-02-14

Total Pages: 271

ISBN-13: 9781493990566

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This detailed book provides state-of-art computational approaches to further explore the exciting opportunities presented by single-cell technologies. Chapters each detail a computational toolbox aimed to overcome a specific challenge in single-cell analysis, such as data normalization, rare cell-type identification, and spatial transcriptomics analysis, all with a focus on hands-on implementation of computational methods for analyzing experimental data. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Computational Methods for Single-Cell Data Analysis aims to cover a wide range of tasks and serves as a vital handbook for single-cell data analysis.