Liquid Chromatography Detectors

Liquid Chromatography Detectors

Author: R.P.W. Scott

Publisher: Elsevier

Published: 1986-02-01

Total Pages: 289

ISBN-13: 0080858368

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The renaissance of liquid chromatography took place in the late 1960's and early 1970's. The first edition of this book published in 1977 described the detectors that were available at that time and which provided a performance matching that of the contemporary equipment with which they were associated. It is interesting to note that the most popular detectors then (the UV detector, the refractometer detector, the fluorescence detector and the electrical conductivity detector) are still the most commonly used detectors nearly a decade later. Detector design, however, has changed very significantly over the intervening years. Modern high efficiency columns provide very narrow peaks and very fast separations, and thus the physical design of the detectors had to change to meet these new challenges. In 1977, there was little real understanding of the important role played by the detector in the overall function of the chromatographic system and although some of the factors were pointed out in the first edition of this book, in retrospect they appeared to be little understood.This second edition gives an entirely new presentation of the subject of liquid chromatography detectors. It contains sections dealing with the fundamental aspects of the interaction between columns and detectors and the interaction between ancillary equipment and the detector. It brings the reader up-to-date with new designs and novel detecting systems that have been developed since 1977 and extends significantly the subject of the association of the liquid chromatography detector with spectroscopic techniques. In particular the book now explores the association of liquid chromatography with nuclear magnetic resonance, infrared and atomic absorption spectrometry. This book not only gives a comprehensive treatment of the subject of liquid chromatographic detectors and provides a rational procedure for defining their performance and so permit valid comparisons, but also discusses detector performance in relation to the whole of the chromatographic system.


Charged Aerosol Detection for Liquid Chromatography and Related Separation Techniques

Charged Aerosol Detection for Liquid Chromatography and Related Separation Techniques

Author: Paul H. Gamache

Publisher: John Wiley & Sons

Published: 2017-05-30

Total Pages: 548

ISBN-13: 0470937785

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The first book devoted exclusively to a highly popular, relatively new detection technique Charged Aerosol Detection for Liquid Chromatography and Related Separation Techniques presents a comprehensive review of CAD theory, describes its advantages and limitations, and offers extremely well-informed recommendations for its practical use. Using numerous real-world examples based on contributors’ professional experiences, it provides priceless insights into the actual and potential applications of CAD across a wide range of industries. Charged aerosol detection can be combined with a variety of separation techniques and in numerous configurations. While it has been widely adapted for an array of industrial and research applications with great success, it is still a relatively new technique, and its fundamental performance characteristics are not yet fully understood. This book is intended as a tool for scientists seeking to identify the most effective and efficient uses of charged aerosol detection for a given application. Moving naturally from basic to advanced topics, the author relates fundamental principles, practical uses, and applications across a range of industrial settings, including pharmaceuticals, petrochemicals, biotech, and more. Offers timely, authoritative coverage of the theory, experimental techniques, and end-user applications of charged aerosol detection Includes contributions from experts from various fields of applications who explore CAD’s advantages over traditional HPLC techniques, as well its limitations Provides a current theoretical and practical understanding of CAD, derived from authorities on aerosol technology and separation sciences Features numerous real-world examples that help relate fundamental properties and general operational variables of CAD to its performance in a variety of conditions Charged Aerosol Detection for Liquid Chromatography and Related Separation Techniques is a valuable resource for scientists who use chromatographic techniques in academic research and across an array of industrial settings, including the biopharmaceutical, biotechnology, biofuel, chemical, environmental, and food and beverage industries, among others.


Chromatographic Detectors

Chromatographic Detectors

Author: Raymond P.W. Scott

Publisher: CRC Press

Published: 1996-07-17

Total Pages: 533

ISBN-13: 148227356X

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"Comprehensively covers the design, construction, and operation of gas chromatography, liquid chromatography, and thin-layer chromatography detectors--all in one convenient, up-to-date source. Emphasizes the essential use of common specifications to describe all detectors, allowing easy comparison of their attributes."


Coulometric Electrode Array Detectors for Hplc

Coulometric Electrode Array Detectors for Hplc

Author: Ian N. Acworth

Publisher: VSP

Published: 1997

Total Pages: 396

ISBN-13: 9789067642323

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This sixth volume in the book series Progress in HPLC-HPCE examines the enhancement of high- performance liquid chromatography through the development of an advanced mode of electrochemical detection (ECD) --- the coulometric array detection --- from its initial, yet problematic, amperometric (thin-layer) design to the highly sensitive, selective and stable coulometric (flow-through) design. Unlike amperometric electrodes, the coulometric electrode is 100% efficient and measures signals from all of the analyte passing through it, which leads to improved sensitivity as well as unique selectivity. The coulometric electrode array offers the resolution of the photodiode array with the extreme sensitivity of an electrochemical detector.


Liquid Chromatography Detectors

Liquid Chromatography Detectors

Author: T. M. Vickrey

Publisher: CRC Press

Published: 1983-07-21

Total Pages: 454

ISBN-13: 9780824719166

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This book documents the principles of operation, the advantages and drawbacks, and the potential future of currently available liquid chromatographic detectors. In offering a snapshot of the current technology, it provides clear explanations and possible new horizons for both beginners and experts.


Pulsed Electrochemical Detection in High-Performance Liquid Chromatography

Pulsed Electrochemical Detection in High-Performance Liquid Chromatography

Author: William R. LaCourse

Publisher: Wiley-Interscience

Published: 1997-08-18

Total Pages: 360

ISBN-13:

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A reliable, comprehensive, relevant view of HPLC and its applications The development of HPLC-PED represents the successful marriage of two powerful analytical technologies and has resulted in the best technique for sensitive and direct detection of biological compounds with poor optical detection properties. PED has been used extensively for the determination of carbohydrates and other polar aliphatic compounds, and as a result, numerous methods have been developed to enable the analysis of a wide variety of samples. Over the years, many articles, anecdotal information, and misinformation have permeated the scientific community, with the possible consequence of confusion or uncertainty with regard to PED on the part of the analyst. Pulsed Electrochemical Detection in High-Performance Liquid Chromatography presents a reliable, comprehensive, and relevant review of HPLC-PED and its applications. The book is divided into three major parts: background material necessary for a more thorough understanding of the principles and relevance of PED; an in-depth discussion of PED using voltammetry and other electroanalytical techniques and presenting the advantages, applicability, and optimization of all existing PED waveforms; and practical aspects of HPLC-PED, including a summary of the major applications and a look at future developments in the technique. Appendices include a pulsed voltammetry (PV) program specifically written to optimize pulsed amperometric detection (PAD) waveforms and all the known applications, categorized and listed in tabular form. For analytical chemists; biochemists; carbohydrate chemists; biotechnologists; undergraduate, graduate, and postdoctoral students; and lab technicians working in a range of areas including the pharmaceutical, medical, and food and beverage industries, this eminently readable guide is the first reliable book-length treatment of how to use PED coupled with HPLC.


Encyclopedia of Chromatography

Encyclopedia of Chromatography

Author: Jack Cazes

Publisher: CRC Press

Published: 2009-10-12

Total Pages: 2968

ISBN-13: 1439870675

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Thoroughly revised and expanded, this third edition offers illustrative tables and figures to clarify technical points in the articles and provides a valuable, reader-friendly reference for all those who employ chromatographic methods for analysis of complex mixtures of substances. An authoritative source of information, this introductory guide to specific chromatographic techniques and theory discusses the relevant science and technology, offering key references for analyzing specific chemicals and applications in industry and focusing on emerging technologies and uses.


Detectors for Liquid Chromatography

Detectors for Liquid Chromatography

Author: Edward S. Yeung

Publisher: Wiley-Interscience

Published: 1986

Total Pages: 392

ISBN-13:

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Presents the state-of-the-art in the design and application of Liquid Chromatography, (LC) detectors. Written by ten experts in the field, the nine chapters cover the refractive index detector, absorption detectors for high-performance liquid chromatography, FTIR detection, indirect absorbance detectors, fluorometric detection, polarimetric detectors, detection based on electrical and electrochemical measurements, mass spectrometry as an on-line detector for HPLC, and more.


Thin-Layer Chromatography with Flame Ionization Detection

Thin-Layer Chromatography with Flame Ionization Detection

Author: M. Ranny

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 197

ISBN-13: 9400937059

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Thin-layer chromatography (TLC) has become a common and much favoured separation technique in laboratories in widely varied fields in recent years. Much of the credit for the introduction of this technique into analytical practice at the l 2 end of the 1950s is due to E. Stahl • • This method is simple and is characterized by high separation ability and sufficient sensitivity3; however, some analysts feel that it has passed the peak in its development and will gradually be replaced by the more modem high-performance liquid chromatography (HPLC). This is undoubtedly a very important analytical technique utilizing the specific separa tion properties of a large number of sorbents and the possibility of regulating 4 the flow-rate of the mobile phase by adjusting the pressure • Standardization of the experimental conditions is simpler in HPLC than in TLC, where the activity of the sorbent and flow-rate of the eitlent in the thin layer depend markedly on the relative humidity of the laboratory atmosphere and on the composition of the gaseous phase in the elution chamber. In addition, systems for quantitative detection of the separated ~ones are better developed for HPLC than for classical TLC, where, until recently, cumbersome and often even insufficiently reproducible chemical or gravimetric analysis of the extracts of scraped-off spots or densitometry of the separated zones, located first by pyrolysis or reaction s with suitable detection agents, were the predominant determination methods .