Cryopreservation in Eukaryotes
Cryopreservation in Eukaryotes

Author: Francisco Marco-Jimenez

Publisher: BoD – Books on Demand

Published: 2016-11-30

Total Pages: 230

ISBN-13: 9535127799

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Since accidentally discovering the ability of glycerol on protecting cells from freezing damage, many researchers have been pursuing to develop cryopreservation methods of a very wide range of cells and some tissues, and these have found widespread applications in biology and medicine. From the point of view of living organisms, cryopreservation is a useful tool for ex situ conservation of genetic resources together with its contribution on conservation of their biodiversity. Cryopreservation in Eukaryotes includes totally 12 chapters, which have been written by the expert researchers in the field. The chapters are a comprehensive collection of the most frequently used methods for eukaryotes. With this book, every researcher will better understand the principles, background, and current status of cryopreservation in particular organisms.

Cryopreservation and Freeze-Drying Protocols
Cryopreservation and Freeze-Drying Protocols

Author: John G. Day

Publisher: Springer Science & Business Media

Published: 2007-06-05

Total Pages: 703

ISBN-13: 1588293777

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In addition to outlining the fundamental principles associated with the conservation of biological resources, freeze-drying and cryopreservation, this text is a compilation of cryptopreservation and freeze-drying methodologies applicable to different biological materiels, developed by expert laboratories.

Plant Cryopreservation: A Practical Guide
Plant Cryopreservation: A Practical Guide

Author: Barbara B.M. Reed

Publisher: Springer Science & Business Media

Published: 2007-10-31

Total Pages: 534

ISBN-13: 0387722769

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Cryopreservation has proven to be an important tool for the storage and conservation of plant genetic resources. This book is a unique resource for plant scientists, providing more than 100 ready-to-use cryopreservation protocols for plant types from algae and bryophytes to a range of flowering plants. It includes techniques for diverse plant parts such as dormant buds, pollen, and apical meristems and for cell types such as suspension and callus cultures.

Cryopreservation
Cryopreservation

Author: Marian Quain

Publisher: BoD – Books on Demand

Published: 2020-06-10

Total Pages: 192

ISBN-13: 1838802053

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Cryopreservation - Current Advances and Evaluations sheds light on storage of cells at subzero temperatures while ensuring that biological functionality is not compromised. Cryopreservation presents a perfect technique by which life can be preserved for posterity. However, there are many challenges to overcome and questions to answer, such as: Are organisms and metabolic systems functioning normally after cooling and thawing? This book provides comprehensive information on cryopreservation with a particular focus on cryoprotectant agents (CPAs). CPAs prevent ice from forming on cryogenically preserved cells, tissues, and organs, but can become toxic at high concentrations. As such, more research is needed to determine their precise mechanisms of action and to develop potential new CPAs that will not compromise the biology of cells. This book is an attempt in this direction.

XIIIth International Symposium on Spermatology
XIIIth International Symposium on Spermatology

Author: Lars Björndahl

Publisher: Springer Nature

Published: 2021-07-22

Total Pages: 370

ISBN-13: 3030662926

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These proceedings of the 2018 XIII International Symposium on Spermatology focus on comparative biology, and encourages discussion and the exchange of ideas. The aim of this Symposium was to provide a unique opportunity and bring together scientists from a wide spectrum of research fields – human, domestic animals and other mammals, vertebrates, insects, and plants. The underlying focus is on the function of the spermatozoon – a common feature for sexual reproduction, but extremely varied. By exploring the variability, a better understanding of male reproductive functions can develop. These proceedings address the mechanisms of physiology and pathophysiology, rather than diagnosis and treatment. The symposium featured keynote lectures by invited speakers, followed by presentations on specific aspects of the general topic of the session. Experimental studies are given priority over clinical studies of patient populations. The proceedings comprise both keynote speakers' texts and selected free communications. Posters were considered for publication in the proceedings, and the volume includes exhibited materials on the work of prominent spermatologists, highlighting their important past achievements in the field.

A Practical Guide to Basic Laboratory Andrology
A Practical Guide to Basic Laboratory Andrology

Author: Lars Björndahl

Publisher: Cambridge University Press

Published: 2010-04-01

Total Pages: 357

ISBN-13: 1139482491

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This practical, extensively illustrated handbook covers the procedures that are undertaken in andrology and ART laboratories to analyse and assess male-factor infertility, and to prepare spermatozoa for use in assisted conception therapy. The content is presented as brief, authoritative overviews of the relevant biological background for each area, plus detailed, step-by-step descriptions of the relevant analytical procedures. Each technical section includes pertinent quality control considerations, as well as the optimum presentation of results. In addition to the comprehensive 'basic' semen analysis, incorporating careful analysis of sperm morphology, the handbook provides established techniques for the use of computer-aided sperm analysis and sperm functional assessment. Throughout the handbook the interpretation of laboratory results in the clinical context is highlighted, and safe laboratory practice is emphasized. It is an invaluable resource to all scientists and technicians who perform diagnostic testing for male-factor infertility.

Basic and Applied Aspects of Biotechnology
Basic and Applied Aspects of Biotechnology

Author: Varsha Gupta

Publisher: Springer

Published: 2016-10-22

Total Pages: 543

ISBN-13: 9811008752

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This book explores the journey of biotechnology, searching for new avenues and noting the impressive accomplishments to date. It has harmonious blend of facts, applications and new ideas. Fast-paced biotechnologies are broadly applied and are being continuously explored in areas like the environmental, industrial, agricultural and medical sciences. The sequencing of the human genome has opened new therapeutic opportunities and enriched the field of medical biotechnology while analysis of biomolecules using proteomics and microarray technologies along with the simultaneous discovery and development of new modes of detection are paving the way for ever-faster and more reliable diagnostic methods. Life-saving bio-pharmaceuticals are being churned out at an amazing rate, and the unraveling of biological processes has facilitated drug designing and discovery processes. Advances in regenerative medical technologies (stem cell therapy, tissue engineering, and gene therapy) look extremely promising, transcending the limitations of all existing fields and opening new dimensions for characterizing and combating diseases.

Experimental Evolution
Experimental Evolution

Author: Theodore Garland

Publisher: Univ of California Press

Published: 2009-12-03

Total Pages: 752

ISBN-13: 0520261801

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This volume summarizes studies in experimental evolution, outlining current techniques and applications, and presenting the field's range of research.

Genetic Damage in Human Spermatozoa
Genetic Damage in Human Spermatozoa

Author: Elisabetta Baldi

Publisher: Springer Science & Business Media

Published: 2013-08-16

Total Pages: 206

ISBN-13: 1461477832

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There are several types of damage that can be found in the male gamete. Genetic damage in spermatozoa can originate during spermatogenesis, or it can originate during transit in both male and female genital tracts. Damage can also be due to ageing, environmental or iatrogenic conditions, as well as to the protocols to cryopreserve and to select spermatozoa in assisted reproduction techniques. The purpose of this book is to provide a comprehensive resource for all possible DNA damages in sperm, the relation to fertility and infertility, and possible transgenerational heritable effects.

Human Stem Cell Technology and Biology
Human Stem Cell Technology and Biology

Author: Gary S. Stein

Publisher: John Wiley & Sons

Published: 2011-03-04

Total Pages: 439

ISBN-13: 0470889896

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Human Stem Cell Technology & Biology: A Research Guide and Laboratory Manual integrates readily accessible text, electronic and video components with the aim of effectively communicating the critical information needed to understand and culture human embryonic stem cells. Key Features: An authoritative, comprehensive, multimedia training manual for stem cell researchers Easy to follow step-by-step laboratory protocols and instructional videos provide a valuable resource A must-have for developing laboratory course curriculums, training courses, and workshops in stem cell biology Perspectives written by the world leaders in the field Introductory chapters will provide background information The volume will be a valuable reference resource for both experienced investigators pursuing stem cell and induced pluripotent stem cell research as well as those new to this field.