Cloning of the Gene for Metallothionein from Artemia and Challenges with Cloning the Promoter
Cloning of the Gene for Metallothionein from Artemia and Challenges with Cloning the Promoter

Author: Gwen R. Jordaan

Publisher:

Published: 2012

Total Pages: 154

ISBN-13: 9781267790552

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Abstract: Four metallothionein (MT) isoforms have previously been identified in the brine shrimp Artemia. The goals of this study were to isolate at least one of the MT genes, clone a MT promoter region, and characterize the cis-acting sequences that regulate MT gene expression. PCR of Artemia genomic DNA amplified a product containing a MT gene with no internal introns. Three different approaches were made to clone the MT promoter. Firstly, conventional cloning of size-selected genomic DNA into a dephosphorylated vector produced empty clones or no clones. The use of a novel linear cloning vector produced many recombinant clones, but screening of the DNA library did not identify a MT promoter sequence. Lastly, genome walking by PCR amplified a sequence that lacked a TATA box and metal response elements. A blast search identified the sequence as a mitochondrial hydroxyacylglutathione hydrolase found in marine organisms.

Cell and Molecular Biology of Artemia Development
Cell and Molecular Biology of Artemia Development

Author: A. Warner

Publisher: Springer Science & Business Media

Published: 2013-06-29

Total Pages: 432

ISBN-13: 1475700040

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The brine shrimp Artemia has become an important experimental system for studies of the developmental process. In recent years the shrimp has yielded considerable information on the pattern of development, bio chemistry, and gene structure and expression of crustaceans. This book is a compilation of research activity from twenty five of the most active re search laboratories working with brine shrimp in the above areas. It also represents the proceedings of a NATO Advanced Research Workshop held in Montreal, Canada, August 11-13, 1988. The book contains twenty nine full papers covering the major areas discussed at the workshop. In addition, one page abstracts representing seventeen poster presentations which were given at the workshop, and which were deemed to be most relevant to the theme of the book, are included. These are designated with an [al in the Table of Contents following the title of each paper. A considerable amount of discussion which took place during the workshop has not been included in the book because of space limitations. However, the editors will endeavour to make some of this in formation available at a later date through the Artemia Newsletter. In addition to the high percentage of invited speakers who attended and contributed to the workshop, the organizers would like to thank a number of participants who made valuable contributions to the major dis cussion sessions. These include: John Freeman, Michael Horst, Herman Slegers, Jack Vaughn, Frank Conte, Sandy McLennan, Clive Trotman and Patrick Sorgeloos.

Handbook of Marine Model Organisms in Experimental Biology
Handbook of Marine Model Organisms in Experimental Biology

Author: Agnes Boutet

Publisher: CRC Press

Published: 2021-12-13

Total Pages: 964

ISBN-13: 1000464334

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The importance of molecular approaches for comparative biology and the rapid development of new molecular tools is unprecedented. The extraordinary molecular progress belies the need for understanding the development and basic biology of whole organisms. Vigorous international efforts to train the next-generation of experimental biologists must combine both levels – next generation molecular approaches and traditional organismal biology. This book provides cutting-edge chapters regarding the growing list of marine model organisms. Access to and practical advice on these model organisms have become a conditio sine qua non for a modern education of advanced undergraduate students, graduate students and postdocs working on marine model systems. Model organisms are not only tools they are also bridges between fields – from behavior, development and physiology to functional genomics. Key Features Offers deep insights into cutting-edge model system science Provides in-depth overviews of all prominent marine model organisms Illustrates challenging experimental approaches to model system research Serves as a reference book also for next-generation functional genomics applications Fills an urgent need for students Related Titles Jarret, R. L. & K. McCluskey, eds. The Biological Resources of Model Organisms (ISBN 978-1-1382-9461-5) Kim, S.-K. Healthcare Using Marine Organisms (ISBN 978-1-1382-9538-4) Mudher, A. & T. Newman, eds. Drosophila: A Toolbox for the Study of Neurodegenerative Disease (ISBN 978-0-4154-1185-1) Green, S. L. The Laboratory Xenopus sp. (ISBN 978-1-4200-9109-0)

Germ Cell Protocols
Germ Cell Protocols

Author: Heide Schatten

Publisher: Springer Science & Business Media

Published: 2008-02-05

Total Pages: 421

ISBN-13: 1592597416

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The study of germ cells has undergone enormous advances in recent years and has entered into an explosive phase of new discoveries with the introd- tion of transgenic technologies and nuclear cloning. Basic knowledge and te- niques developed for lower vertebrate and invertebrate systems have facilitated the study of higher vertebrates, including humans. Many experiments that have first been performed on lower vertebrates provided the tools and strategies that could later be applied to other less readily available mammalian systems. The discovery of centrosomes in ascidians and sea urchin eggs now benefits st- ies of fertility and infertility in mammals including humans. External in vitro fertilization, now a common technique in assisted fertilization has only been possible as a result of numerous studies in lower systems in which external fertilization is natural. Egg activation, first explored in sea urchin and asc- ian eggs, now benefits cloning efficiency in farm and domestic animals. Gene manipulations and molecular methods have added to the possibilities of p- ducing live offspring with enormous biomedical, ecological, and economic implications. All sexually reproducing organisms produce primordial germ cells, a small population of cells that differentiate into gametes of either sex that carry to- potency, an ability to develop into an entire new organism. The two volumes on germ cells combine techniques in a variety of different systems and have selected those systems that have provided landmarks in advancing our kno- edge on germ cells.

An Introduction to Ecological Genomics
An Introduction to Ecological Genomics

Author: Nico M. van Straalen

Publisher: Oxford University Press

Published: 2012

Total Pages: 374

ISBN-13: 0199594686

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The authors also provide a comparative survey of the properties of genomes (genome size, gene families, synteny, and polymorphism) for prokaryotes as well as the main eukaryotic models.

Cellular Effects of Heavy Metals
Cellular Effects of Heavy Metals

Author: Gaspar Banfalvi

Publisher: Springer Science & Business Media

Published: 2011-03-02

Total Pages: 348

ISBN-13: 9400704283

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The term “heavy metals” is used as a group name of toxic metals and metalloids (semimetals) causing contaminations and ecotoxicity. In strict chemical sense the density of heavy metals is higher than 5 g/cm3. From biological point of view as microelements they can be divided into two major groups. a. For their physiological function organisms and cells require essential microelements such as iron, chromium (III), cobalt, copper, manganese, molidenium, zinc. b. The other group of heavy metals is toxic to the health or environment. Of highest concern are the emissions of As, Cd, Co, Cu, Hg, Mn, Ni, Pb, Sn, Tl. The toxicity of heavy metals is well known at organizational level, while less attention has been paid to their cellular effects. This book describes the toxicity of heavy metals on microorganisms, yeast, plant and animal cells. Other chapters of the book deal with their genotoxic, mutagenic and carcinogenic effects. The toxicity of several metals touch upon the aspects of environmental hazard, ecosystems and human health. Among the cellular responses of heavy metals irregularities in cellular mechanisms such as gene expression, protein folding, stress signaling pathways are among the most important ones. The final chapters deal with biosensors and removal of heavy metals. As everybody is eating, drinking and exposed to heavy metals on a daily basis, the spirit of the book will attract a wide audience.

Microorganisms In Industry And Environment: From Scientific And Industrial Research To Consumer Products - Proceedings Of The Iii International Conference On Environmental, Industrial And Applied Microbiology (Biomicroworld2009)
Microorganisms In Industry And Environment: From Scientific And Industrial Research To Consumer Products - Proceedings Of The Iii International Conference On Environmental, Industrial And Applied Microbiology (Biomicroworld2009)

Author: Antonio Mendez-vilas

Publisher: World Scientific

Published: 2010-12-09

Total Pages: 736

ISBN-13: 9814464198

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This book aims to disseminate the most current research in applied microbiology presented at the III International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2009) held in Lisbon, Portugal, in December 2009.This volume offers an inviting exploration of microbiology from scientific and industrial research to consumer products in a compilation of more than 150 papers written by leading experts in the field, who afford critical insights into several topics, review current research and discuss future directions to stimulate further discussions. This book also serves as an update on the most important current microbial research, by providing a comprehensive overview of cutting-edge topics in a single volume, where readers can also gain insights into how microbiology can solve problems in everyday settings.Although largely intended for microbiologists interested in knowing the latest developments in agriculture, environmental, food, industrial, medical and pharmaceutical microbiology and microbial biotechnology, this book is also a great source of reference for scientists and researchers involved in advancements in applied microbiology.