Cloning and Analysis of an Avian Metallothionein Coding Gene and Mapping of Metal Responsive Elements Within the Promoter
Author: Lawrence P. Fernando
Publisher:
Published: 1990
Total Pages: 416
ISBN-13:
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Author: Lawrence P. Fernando
Publisher:
Published: 1990
Total Pages: 416
ISBN-13:
DOWNLOAD EBOOKAuthor: Gwen R. Jordaan
Publisher:
Published: 2012
Total Pages: 154
ISBN-13: 9781267790552
DOWNLOAD EBOOKAbstract: Four metallothionein (MT) isoforms have previously been identified in the brine shrimp Artemia. The goals of this study were to isolate at least one of the MT genes, clone a MT promoter region, and characterize the cis-acting sequences that regulate MT gene expression. PCR of Artemia genomic DNA amplified a product containing a MT gene with no internal introns. Three different approaches were made to clone the MT promoter. Firstly, conventional cloning of size-selected genomic DNA into a dephosphorylated vector produced empty clones or no clones. The use of a novel linear cloning vector produced many recombinant clones, but screening of the DNA library did not identify a MT promoter sequence. Lastly, genome walking by PCR amplified a sequence that lacked a TATA box and metal response elements. A blast search identified the sequence as a mitochondrial hydroxyacylglutathione hydrolase found in marine organisms.
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Published: 1991
Total Pages: 796
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Published: 1984
Total Pages: 1100
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Publisher:
Published: 1990
Total Pages: 1254
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Publisher:
Published: 1990
Total Pages: 736
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Published: 1995
Total Pages: 480
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Published: 1990-12
Total Pages: 596
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Published: 1996
Total Pages: 1268
ISBN-13:
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