Cell Separation
Cell Separation

Author: Thomas G. Pretlow

Publisher: Academic Press

Published: 2014-05-10

Total Pages: 343

ISBN-13: 1483219380

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Cell Separation: Methods and Selected Applications is a compendium of articles on the design and/or application of methods for the separation of cells. This volume presents contributions on relatively finite subjects on cell separation. It covers topics on cell separation such as methods for obtaining cells in suspension from animal tissues; some of the kinds of data that are helpful in the description of cell purifications; and separation of host cells infiltrating tumors and allografts by velocity sedimentation at unit gravity. The separation of different kinds of nucleated cells from blood by centrifugal elutriation; a new approach to the separation of cells at unit gravity; and the isolation and culture of homogeneous populations of glomerular cell types are elucidated as well. Experimental oncologists, hematologists, immunologists, cell biologists, endocrinologists, and others who are not already expert in the use of methods for cell separation will find the book highly useful.

Cell Separation Methods and Applications
Cell Separation Methods and Applications

Author: Diether Recktenwald

Publisher: CRC Press

Published: 1997-11-04

Total Pages: 352

ISBN-13: 1482273640

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"Offers complete coverage and assessment of cell separation technologies for analytical and preparative isolations of biological cell populations-demonstrating how to select and devise optimal sorting strategies for applications in biochemistry, immunology, cell and molecular biology, and clinical research. "

Cell Separation Science and Technology
Cell Separation Science and Technology

Author: Dhinakar S. Kompala

Publisher:

Published: 1991

Total Pages: 320

ISBN-13:

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This new volume provides theoretical and practical information on the state of the science of cell separation, including applications to biotechnology. Presentations from the world's leading authorities cover the four principal modern methods: flow sorting and optical methods; sedimentation and flow fractionation; affinity adsorption and extraction methods; and electrophoresis and magnetic methods. An introductory chapter describes the rationale for separating living cells, the parameters within which separation methods are evaluated, and some physical characteristics of each of the methods reported in the volume. Readers will be able to choose, evaluate, and in some cases, build cell separation procedures and equipment for their own purposes based on the contents of the book, which also covers many applications of each method.

Cell Separation
Cell Separation

Author: Thomas G. Pretlow

Publisher: Academic Press

Published: 2014-05-10

Total Pages: 344

ISBN-13: 1483219399

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Cell Separation: Methods and Selected Applications, Volume 2 provides information pertinent to the design and application of methods for the separation of cells. This book covers a variety of topics, including thyroid structure and function, procurement of eosinophils, elutriation, stromal cells, isolation of epithelial cells, and separation of cells. Organized into 15 chapters, this volume begins with an overview of the structure of thyroid parenchyma and the properties of endocrine parafollicular cells. This text then summarizes the various aspects of the separation methodology to assist investigators with the use of elutriation. Other chapters describe the methods that are useful for the separation of stromal cells from tumors. This book discusses as well the types of methods that are available for the purification of cells from tumors. The final chapter deals with the biological significance of the erythroblastic nest. This book is a valuable resource for cell biologists, experimental oncologists, hematologists, immunologists, and endocrinologists.

Human Naïve Pluripotent Stem Cells
Human Naïve Pluripotent Stem Cells

Author: Peter Rugg-Gunn

Publisher: Humana

Published: 2021-12-07

Total Pages: 288

ISBN-13: 9781071619070

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This volume provides readers with a comprehensive collection of methods to guide them on how to generate, characterize, and use naïve human pluripotent stem cells (hPSCs). The chapters in this book cover topics such as three predominant routes to generate naïve hPSC lines; methods to differentiate naïve hPSCs into specialized cell types; and techniques to characterize naïve hPSCs using key molecular landmarks that benchmark and quality control the cell lines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Human Naïve Pluripotent Stem Cells: Methods and Protocols is a valuable resource for novice and expert researchers who are looking to learn more or expand their research in this developing field.