This cutting-edge book presents protocols and strategies for proteomic evaluation of cardiovascular disease written by pioneering researchers in the field. Topics explored in this comprehensive volume include obtaining specific heart proteins, techniques for identifying risk biomarkers of atherome plaque rupture, analyzing the secretome of explanted endarterectomies cultured in vitro, and phage display techniques for deciphering the molecular diversity of blood vessels.
This book fulfils the need to keep up with the high number of innovations in proteomics, and at the same time to warn the readers about the danger of manufacturers and scientists claims around new technologies. Mass spectrometry stands as the core technology in proteomics. The emerging field of targeted proteomics and its potential applications in the cardiovascular arena are also reviewed and discussed. A concluding section highilghts the promise of proteomics in the light of these recent developments. As this technique and its applications have undergone remarkable advances in the past years, recent updates on proteomic applications are covered. Another key concept revealed by proteomic technologies is that the extent of protein post-translational modifications (PTMs) as well as their impact on the phenotype has been underestimated by pre-proteomics science. As such, part of the manual focuses on the emerging role of PTMs in basic cardiovascular sciences and in the clinics. In fact, there is an emerging consensus that the detailed annotation of protein PTMs could lead to a more in-depth representation of biological systems, translating into more specific targets for therapy as well as biomarkers. Moreover, a recent trend is so-called “targeted proteomics”. The approach was awarded the title of “Method of the Year” by Nature in 2013 (see the editorial by Vivien Marx in 1st issue of Nature in Jan 2013). According to a few proteomic scientists the emphasis should not be placed on generating long lists of proteins but lists of proteins with a true biological meaning.
This is the very first book to focus on this new approach that will eventually aid in developing new diagnostic markers and therapies for controlling and treating heart disease - the number-one killer in the industrialized world. Divided into two parts, the book describes not only the potentials, but also the limitations of these technologies. The editors, both well known within the scientific community, provide new insights into the biochemical and cellular mechanisms of cardiovascular disease, as well as covering the transition into clinical applications. In so doing, they highlight the various strategies and technical aspects so as to assist the growing number of researchers intending to utilize these approaches. The result is an excellent way of educating and informing graduate students, post-doctoral fellows as well as researchers in academia and industry about the latest developments in this area.
Precision Medicine for Investigators, Practitioners and Providers addresses the needs of investigators by covering the topic as an umbrella concept, from new drug trials to wearable diagnostic devices, and from pediatrics to psychiatry in a manner that is up-to-date and authoritative. Sections include broad coverage of concerning disease groups and ancillary information about techniques, resources and consequences. Moreover, each chapter follows a structured blueprint, so that multiple, essential items are not overlooked. Instead of simply concentrating on a limited number of extensive and pedantic coverages, scholarly diagrams are also included. - Provides a three-pronged approach to precision medicine that is focused on investigators, practitioners and healthcare providers - Covers disease groups and ancillary information about techniques, resources and consequences - Follows a structured blueprint, ensuring essential chapters items are not overlooked
The purpose of the book is to introduce platelets, and their functional role in thrombotic and cardiovascular disease, justifying the relevance of platelet proteomics research. Focus then shifts to the recent developments on mass spectrometry (MS)-based proteomics. This chapter shows potential applications for platelet proteomics not yet carried out. It includes examples of post-translational modifications (PTMs) analysis in platelets. The second part of the book focuses on the main research done so far on platelet proteomics. This includes general proteome mapping by non-gel based separation methods (MudPit), analysis of the general platelet proteome and signaling cascades by gel-based separation methods (2-DE), sub-proteome analyses (secretome/releasate, membrane proteins, organelles). Finally, the last section links the platelet transcriptome and application to disease. This section is highly relevant and includes chapters on proteomics, transcriptomics, functional genomics, systems biology, and their applications to platelet-related diseases.
In the four pages committed to a discussion of myocardial infarction in the first edition of Harrison’s Principles of Internal Medicine, published in 1950, there was no mention of use of the laboratory for management of patients. Thirty years later, when the first edition of Braunwald’s Heart Disease, A Textbook of Cardiovascular Medicine was published, 2 out of the 1943 pages in the text contained a discussion of the laboratory examinations in acute myocardial infarction. Our knowledge base of the multitude of ways that physicians can and should use the clinical chemistry laboratory has expanded dramatically since these classic texts were published. The nomenclature has changed: terms such as “cardiac enzymes” have given way to “cardiac biomarkers. ” The number of assays has multiplied, and the operating characteristics of available assays are impr- ing at a gratifying but dizzying rate. We now use biomarkers to diagnose cardiovascular diseases and also to frame our treatment strategies. Thus, there is a clear need for a scholarly compilation of the state of the art of cardiac biomarkers. Dr. David Morrow has expertly edited an authoritative book that answers this need. The 34 chapters in Cardiovascular Biomarkers: Pathophysiology and Disease Mana- ment were written by a group of individuals who are internationally recognized thought leaders and experts in clinical and laboratory medicine.
Biomedical research has entered a new era of characterizing a disease or a protein on a global scale. In the post-genomic era, Proteomics now plays an increasingly important role in dissecting molecular functions of proteins and discovering biomarkers in human diseases. Mass spectrometry, two-dimensional gel electrophoresis, and high-density antibody and protein arrays are some of the most commonly used methods in the Proteomics field. This book covers four important and diverse areas of current proteomic research: Proteomic Discovery of Disease Biomarkers, Proteomic Analysis of Protein Functions, Proteomic Approaches to Dissecting Disease Processes, and Organelles and Secretome Proteomics. We believe that clinicians, students and laboratory researchers who are interested in Proteomics and its applications in the biomedical field will find this book useful and enlightening. The use of proteomic methods in studying proteins in various human diseases has become an essential part of biomedical research.
Inflammation in Heart Failure, edited by W. Matthijs Blankesteijn and Raffaele Altara, is the first book in a decade to provide an in-depth assessment on the causes, symptoms, progression and treatments of cardiac inflammation and related conditions. This reference uses two decades of research to introduce new methods for identifying inflammatory benchmarks from early onset to chronic heart failure and specifically emphasizes the importance of classifying at-risk subgroups within large populations while determining the patterns of cytokines in such classifications. Further, the book details clinical applications of the pathophysiological mechanisms of heart failure, diagnosis and therapeutic strategies. Inflammation in Heart Failure's breadth of subject matter, easy-to-follow structure, portability, and high-quality illustrations create an accessible benefit for researchers, clinicians and students. - Presents updated information and research on the relevant inflammatory mediators of heart failure to aid in targeting future translational research as well as the improvement of early diagnosis and treatment - Provides research into better understanding the different inflammatory mediators that signal the underlying diseases that potentially lead to heart failure - Contains 20 years of research, offering a brief overview of the topic leading to current opinions on, and treatment of, heart failure - Provides a structured, systematic and balanced overview of the role of inflammation in heart failure making it a useful resource for researchers and clinicians, as well as those studying cardiovascular diseases
Get a quick, expert overview of the ways in which biomarkers can be used to assess and guide the management of cardiovascular disease in the clinical setting. This concise, clinically-focused resource by Dr. Vijay Nambi consolidates today's available information on this rapidly changing topic into one convenient resource, making it an ideal, easy-to-digest reference for cardiology practitioners, fellows, and residents. - Covers lab standards and statistical interpretation of biomarkers with a clinical focus. - Discusses relevant conditions such as hypertension and diabetes as key markers of injury and prognosis. - Includes current information on biomarkers to assess and guide the management of heart failure, acute coronary syndrome, chest pain, shortness of breath, and more. - Concludes the book with a timely chapter on how biomarkers may guide cardiologists in the future.
This book contains examples written by internationally recognized experts applying proteomics and analysis applications of mass spectrometry in their everyday research. They dedicate this book to a broad audience of all scientists to encourage them to use the omics approach to investigate biological processes.The first chapter is a short introduction to the basics of analytical chemistry. The following chapters describe high-throughput proteomic studies and how they are applied in various areas of biological and biomedical sciences, providing valuable insights into how biological systems work at the molecular level.
