The book is structured in nine sections, each containing several chapters. The volume starts with an overview of analytical techniques and progresses through purification of proteins; protein modification and inactivation; protein size, shape, and structure; enzyme kinetics; protein-ligand interactions; industrial enzymology; and laboratory quality control. The book is targeted at all scientists interested in protein research.
Proteins: Concepts in Biochemistry teaches the biochemical concepts underlying protein structure, evolution, stability, folding, and enzyme kinetics, and explains how interactions in macromolecular structures determine protein function. Intended for a one-semester course in biochemistry or biophysical chemistry with a focus on proteins, this textbo
Biophysical Characterization of Proteins in Developing Biopharmaceuticals, Second Edition, presents the latest on the analysis and characterization of the higher-order structure (HOS) or conformation of protein based drugs. Starting from the very basics of protein structure, this book explains the best way to achieve this goal using key methods commonly employed in the biopharmaceutical industry. This book will help today's industrial scientists plan a career in this industry and successfully implement these biophysical methodologies. This updated edition has been fully revised, with new chapters focusing on the use of chromatography and electrophoresis and the biophysical characterization of very large biopharmaceuticals. In addition, best practices of applying statistical analysis to biophysical characterization data is included, along with practical issues associated with the concept of a biopharmaceutical's developability and the technical decision-making process needed when dealing with biophysical characterization data. - Presents basic protein characterization methods and tools applicable to (bio)pharmaceutical research and development - Highlights the capabilities and limitations of each technique - Discusses the underlining science of each tool - Empowers industrial biophysical chemists by providing a roadmap for applying biophysical tools - Outlines the needs for new characterization and analytical tools in the biopharmaceutical industry
In the post-genomic world, advances in the comprehension of cell behaviour will depend upon scientists deciphering the molecular basis of interactions between proteins and membranes. Bringing together contributions from chemists, biologists and physicists, Biophysical Chemistry: Membranes and Proteins demonstrates how multidisciplinary teams can gain insights into understanding complex biological systems. This book reflects both the scope and the interdisciplinary nature of the field, with topics including: modelling of biological systems; membrane structure and interactions; probing biomolecules; and channels and receptors. Full of stimulating articles and opinions, readers from academia and industry will welcome the wide range of coverage and the state-of-the-art science.
The second edition of Structure in Protein Chemistry showcases the latest developments and innovations in the field of protein structure analysis and prediction. The book begins by explaining how proteins are purified and describes methods for elucidating their sequences of amino acids and defining their posttranslational modifications. Comprehensive explanations of crystallography and of noncovalent forces-ionic interactions, hydrogen bonding, and the hydrophobic effect-act as a prelude to an exhaustive description of the atomic details of the structures of proteins. The resulting understanding of protein molecular structure forms the basis for discussions of the evolution of proteins, the symmetry of the oligomeric associations that produce them, and the chemical, mathematical, and physical basis of the techniques used to study their structures. The latter include image reconstruction, nuclear magnetic resonance spectroscopy, proton exchange, optical spectroscopy, electrophoresis, covalent cross-linking, chemical modification, immunochemistry, hydrodynamics, and the scattering of light, X-radiation, and neutrons. These procedures are applied to study the folding of polypeptides and the assembly of oligomers. Biological membranes and their proteins are also discussed. Structure in Protein Chemistry, Second Edition, bridges the gap between introductory biophysical chemistry courses and research literature. It serves as a comprehensive textbook for advanced undergraduates and graduate students in biochemistry, biophysics, and structural and molecular biology. Professionals engaged in chemical, biochemical, and molecular biological research will find it a useful reference.
Biophysical Chemistry, Volume I: Thermodynamics, Electrostatics, and the Biological Significance of the Properties of Matter focuses on the biological aspects of the properties of matter, putting emphasis on the chemical elements, water and carbon dioxide, complex molecules, and proteins. The publication first elaborates on biochemistry and geochemistry, water and its biological significance, and the problems of protein structure. Discussions focus on the number of peptide chains in the molecule and nature of terminal groups, latent heat of fusion, characteristics of the amino acids derived from proteins, expansion of water in freezing, and the relative abundance of chemical elements in the universe. The text then takes a look at thermodynamics and the application to polar molecules and ionic solutions of electrostatics, including free energy of a charged sphere, image charges, salting-out effect, expressions for the change of fundamental thermodynamic functions, and chemical potentials. The book examines the conductivity of electrolytes, acid-base equilibria, and polybasic acids, bases, and ampholytes, including proteins. Topics include ionization of cysteine, isoelectric points of polyvalent ampholytes, hemoglobin, nature of acids and bases, measurement of conductivity, electrolytes as conductors, and the moving boundary method of determining transference numbers. The manuscript is a dependable reference for chemists and researchers interested in thermodynamics, electrostatics, and the biological value of the properties of matter.
Organized on a combined basis of chronology and of structural and functional hierarchy, This comprehensive text describes all aspects of proteins--biosynthesis, evolution, dynamics, ligand binding, catalysis, and energy transduction--not just their structures. This edition (first was 1984) is thoroughly updated--especially in the area of protein biosynthesis--and features end-of-chapter exercises and problems, many of which require the student to consult the cited literature in order to obtain the answer. Annotation copyright by Book News, Inc., Portland, OR
DNA, RNA and proteins are undoubtedly the most important biological molecules. Being large macromolecules, their physical, chemical and biological properties can differ from those of the monomers from which they are made. This book describes their structures, origins and other key issues.
Proteins: Structure and Function is a comprehensive introduction to the study of proteins and their importance to modern biochemistry. Each chapter addresses the structure and function of proteins with a definitive theme designed to enhance student understanding. Opening with a brief historical overview of the subject the book moves on to discuss the ‘building blocks’ of proteins and their respective chemical and physical properties. Later chapters explore experimental and computational methods of comparing proteins, methods of protein purification and protein folding and stability. The latest developments in the field are included and key concepts introduced in a user-friendly way to ensure that students are able to grasp the essentials before moving on to more advanced study and analysis of proteins. An invaluable resource for students of Biochemistry, Molecular Biology, Medicine and Chemistry providing a modern approach to the subject of Proteins.
The first of its kind, Introduction to Biophysical Methods for Protein and Nucleic Acid Research serves as a text for the experienced researcher and student requiring an introduction to the field. Each chapter presents a description of the physical basis of the method, the type of information that may be obtained with the method, how data should be analyzed and interpreted and, where appropriate, practical tips about procedures and equipment.Key Features* Modern Use of Mass Spectroscopy* NMR Spectroscopy* Molecular Modeling and Graphics* Macintosh and DOS/Windows 3.x disks
