The field of Clinical Microbiology is evolving at a rapid pace, perhaps more so than any other arm of laboratory medicine. This can be attributed to new technology, including high throughput gene sequencing, multiplex molecular assays, rapid evolution of antimicrobial resistance, and discovery of new pathogens. In addition, modern medical procedures, such as solid organ and stem cell transplantation, have resulted in an explosion of infections with agents that historically have been considered to be of low virulence. This issue of Clinics in Laboratory Medicine will highlight some of the advances in diagnostic microbiology, including MALDI-TOF MS, pathogen discovery, and personalized antimicrobial chemotherapy. In addition, one of the papers will focus on implementation of new technologies and how to maximize patient impact of these new methods.
Current and Emerging Technologies in Microbial Diagnostics, the latest volume in the Methods in Microbiology series, provides comprehensive, cutting-edge reviews of current and emerging technologies in the field of clinical microbiology. The book features a wide variety of state-of-the art methods and techniques for the diagnosis and management of microbial infections, with chapters authored by internationally renowned experts. This volume focuses on current techniques, such as MALDI-TOF mass spectroscopy and molecular diagnostics, along with newly emerging technologies such as host-based diagnostics and next generation sequencing. - Written by recognized leaders and experts in the field - Provides a comprehensive and cutting-edge review of current and emerging technologies in the field of clinical microbiology, including discussions of current techniques such as MALDI-TOF mass spectroscopy and molecular diagnostics - Includes a broad range and breadth of techniques covered - Presents discussions on newly emerging technologies such as host-based diagnostics and next generation sequencing
Perfect your lab skills with the gold standard in microbiology! Serving as both the #1 bench reference for practicing microbiologists and as a favorite text for students in clinical laboratory science programs, Bailey & Scott's Diagnostic Microbiology, 14th Edition covers all the topical information and critical thinking practice you need for effective laboratory testing. This new edition also features hundreds step-by-step procedures, updated visuals, new case studies, and new material on the latest trends and equipment in clinical microbiology — including automation, automated streaking, MALDI-TOF, and incubator microscopes. It's everything you need to get quality lab results in class and in clinical practice! - More than 800 detailed, full-color illustrations aid comprehension and help in visualizing concepts. - Expanded sections on parasitology, mycology, and virology eliminate the need to purchase separate books on this material. - General and Species boxes in the organism chapters highlight the important topics that will be discussed in the chapter. - Case studies provide the opportunity to apply information to a variety of diagnostic scenarios, and help improve decision-making and critical thinking skills. - Hands-on procedures include step-by-step instructions, full-color photos, and expected results. - A glossary of terms is found at the back of the book for quick reference. - Learning objectives begin each chapter, offering a measurable outcome to achieve by the completing the material. - Learning resources on the Evolve companion website enhance learning with review questions and procedures. - NEW! Coverage of automation, automated streaking, MALDI-TOF, and incubator microscopes keeps you in the know on these progressing topics. - NEW! Updated images provide a more vivid look into book content and reflect the latest procedures. - NEW! Thoroughly reviewed and updated chapters equip you with the most current information. - NEW! Significant lab manual improvements provide an excellent learning resource at no extra cost. - NEW! 10 extra case studies on the Evolve companion website offer more opportunities to improve critical thinking skills.
Infectious Disease Epidemiology is a concise reference guide which provides trainees and practicing epidemiologists with the information that they need to understand the basic concepts necessary for working in this specialist area. Divided into two sections, part one comprehensively covers the basic principles and methods relevant to the study of infectious disease epidemiology. It is organised in order of increasing complexity, ranging from a general introduction to subjects such as mathematical modelling and sero-epidemiology. Part two examines key major infectious diseases that are of global significance. Grouped by their route of transmission for ease of reference, they include diseases that present a particular burden or a high potential for causing mortality. This practical guide will be essential reading for postgraduate students in infectious disease epidemiology, health protection trainees, and practicing epidemiologists.
The chapters of this book describe numerous successful examples of automation in microbiology, e.g., radiometric detection of bacteremia, instruments for detection of bacteriuria, machines for organism identification and susceptibility testing, and automated antigen and antibody measurement systems. In addition, there are discussions of exciting but not yet proven methodologies such as chromatography, flow cytometry, and other applications of radiometry. There are also important discussions regarding improved means of data communication and ways to improve the clinician‘s use of test results. Lastly, there are candid assessments of the best and worst aspects of the current spectrum of automated instruments for microbiology. It is hoped that the reader of this volume will be left with a feeling of excitement at the possibilities that lie ahead for application of instrument techniques in the diagnosis of infectious diseases.
The Manual of Commercial Methods in Clinical Microbiology 2nd Edition, International Edition reviews in detail the current state of the art in each of the disciplines of clinical microbiology, and reviews the sensitivities, specificities and predictive values, and subsequently the effectiveness, of commercially available methods – both manual and automated. This text allows the user to easily summarize the available methods in any particular field, or for a specific pathogen – for example, what to use for an Influenza test, a Legionella test, or what instrument to use for identification or for an antibiotic susceptibility test. The Manual of Commercial Methods in Clinical Microbiology, 2nd Edition, International Edition presents a wealth of relevant information to clinical pathologists, directors and supervisors of clinical microbiology, infectious disease physicians, point-of-care laboratories, professionals using industrial applications of diagnostic microbiology and other healthcare providers. The content will allow professionals to analyze all commercially available methods to determine which works best in their particular laboratory, hospital, clinic, or setting. Updated to appeal to an international audience, The Manual of Commercial Methods in Clinical Microbiology, 2nd Edition, International Edition is an invaluable reference to those in the health science and medical fields.
The chapters of this book describe numerous successful examples of automation in microbiology, e.g., radiometric detection of bacteremia, instruments for detection of bacteriuria, machines for organism identification and susceptibility testing, and automated antigen and antibody measurement systems. In addition, there are discussions of exciting but not yet proven methodologies such as chromatography, flow cytometry, and other applications of radiometry. There are also important discussions regarding improved means of data communication and ways to improve the clinician‘s use of test results. Lastly, there are candid assessments of the best and worst aspects of the current spectrum of automated instruments for microbiology. It is hoped that the reader of this volume will be left with a feeling of excitement at the possibilities that lie ahead for application of instrument techniques in the diagnosis of infectious diseases.
This book discusses principles, methodology, and applications of microbiological laboratory techniques . It lays special emphasis on the use of various automated machines that are essential for medical microbiology and diagnostic labs. The book contains eleven major chapters. The first chapter describes the good lab practices which should be followed by the students in all biological, chemistry or microbiology laboratories. The next chapter describes manual and automated characterization of antibiotic resistant microbes, followed by a chapter on genomics based tools and techniques that are integral to research. Further chapters deal with other important techniques like immunology based techniques, spectrophotometry and its various types, MALDI-TOFF and microarrays, each with illustrations and detailed description of the protocols and applications. The book also gives certain important guidelines to the students about the planning the experiment and interpreting results. The book is highly informative and provides latest techniques. It is a handy compendium for graduate and post graduate students, as well as more advanced researchers.
The Use of Mass Spectrometry Technology (MALDI-TOF) in Clinical Microbiology presents the state-of the-art for MALDI-TOF mass spectrometry. It is a key reference defining how MALDI-TOF mass spectrometry is used in clinical settings as a diagnostic tool of microbial identification and characterization that is based on the detection of a mass of molecules. The book provides updated applications of MALDI-TOF techniques in clinical microbiology, presenting the latest information available on a technology that is now used for rapid microbial identification at relatively low cost, thus offering an alternative to conventional laboratory diagnosis and proteomic identification systems. Although the main use of the technology has, until now, been identification or typing of bacteria from a positive culture, applications in the field of virology, mycology, microbacteriology and resistances are opening up new opportunities. - Presents updated applications of MALDI-TOF techniques in clinical microbiology - Describes the use of mass spectrometry in the lab, the principles of the technology, preparation of samples, device calibration and maintenance, treatment of microorganisms, and quality control - Presents key information for researchers, including possible uses of the technology, differences between devices, how to interpret results, and future applications - Covers the topic in a systematic and comprehensive manner that is useful to both clinicians and researchers
